Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8
Strigolactones (SLs), a group of plant hormones, induce germination of root-parasitic plants and inhibit shoot branching in many plants. Shoot branching is an important trait that affects the number and quality of flowers and fruits. Root-parasitic plants, such as Phelipanche spp., infect tomato roo...
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| Language: | English |
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MDPI AG
2018-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/19/9/2645 |
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doaj-art-a5847c7f5bed496783f39c1262e12f072018-09-07T07:06:39ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-09-01199264510.3390/ijms19092645ijms19092645Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8Shoko Hasegawa0Takuya Tsutsumi1Shunsuke Fukushima2Yoshihiro Okabe3Junna Saito4Mina Katayama5Masato Shindo6Yusuke Yamada7Koichiro Shimomura8Kaori Yoneyama9Kohki Akiyama10Koh Aoki11Tohru Ariizumi12Hiroshi Ezura13Shinjiro Yamaguchi14Mikihisa Umehara15Graduate School of Life Sciences, Toyo University, Itakura-machi, Ora-gun, Gunma 374-0193, JapanDepartment of Applied Biosciences, Toyo University, Itakura-machi, Ora-gun, Gunma 374-0193, JapanDepartment of Applied Biosciences, Toyo University, Itakura-machi, Ora-gun, Gunma 374-0193, JapanFaculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, JapanGraduate School of Life and Environmental Sciences, Osaka Prefecture University, Naka-ku, Sakai, Osaka 599-8531, JapanCollege of Life, Environment, and Advanced Sciences, Osaka Prefecture University, Naka-ku, Sakai, Osaka 599-8531, JapanGraduate School of Life Sciences, Toyo University, Itakura-machi, Ora-gun, Gunma 374-0193, JapanGraduate School of Life Sciences, Toyo University, Itakura-machi, Ora-gun, Gunma 374-0193, JapanGraduate School of Life Sciences, Toyo University, Itakura-machi, Ora-gun, Gunma 374-0193, JapanGraduate School of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, JapanGraduate School of Life and Environmental Sciences, Osaka Prefecture University, Naka-ku, Sakai, Osaka 599-8531, JapanGraduate School of Life and Environmental Sciences, Osaka Prefecture University, Naka-ku, Sakai, Osaka 599-8531, JapanFaculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, JapanFaculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, JapanGraduate School of Life Sciences, Tohoku University, Katahira, Aoba-ku, Sendai 980-8577, JapanGraduate School of Life Sciences, Toyo University, Itakura-machi, Ora-gun, Gunma 374-0193, JapanStrigolactones (SLs), a group of plant hormones, induce germination of root-parasitic plants and inhibit shoot branching in many plants. Shoot branching is an important trait that affects the number and quality of flowers and fruits. Root-parasitic plants, such as Phelipanche spp., infect tomato roots and cause economic damage in Europe and North Africa—hence why resistant tomato cultivars are needed. In this study, we found carotenoid cleavage dioxygenase 8-defective mutants of Micro-Tom tomato (slccd8) by the “targeting induced local lesions in genomes” (TILLING) method. The mutants showed excess branching, which was suppressed by exogenously applied SL. Grafting shoot scions of the slccd8 mutants onto wild-type (WT) rootstocks restored normal branching in the scions. The levels of endogenous orobanchol and solanacol in WT were enough detectable, whereas that in the slccd8 mutants were below the detection limit of quantification analysis. Accordingly, root exudates of the slccd8 mutants hardly stimulated seed germination of root parasitic plants. In addition, SL deficiency did not critically affect the fruit traits of Micro-Tom. Using a rhizotron system, we also found that Phelipanche aegyptiaca infection was lower in the slccd8 mutants than in wild-type Micro-Tom because of the low germination. We propose that the slccd8 mutants might be useful as new tomato lines resistant to P. aegyptiaca.http://www.mdpi.com/1422-0067/19/9/2645carotenoid cleavage dioxygenase 8Orobanche minorPhelipanche aegyptiacashoot branchingSolanum lycopersicumstrigolactones |
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| language |
English |
| format |
Article |
| author |
Shoko Hasegawa Takuya Tsutsumi Shunsuke Fukushima Yoshihiro Okabe Junna Saito Mina Katayama Masato Shindo Yusuke Yamada Koichiro Shimomura Kaori Yoneyama Kohki Akiyama Koh Aoki Tohru Ariizumi Hiroshi Ezura Shinjiro Yamaguchi Mikihisa Umehara |
| spellingShingle |
Shoko Hasegawa Takuya Tsutsumi Shunsuke Fukushima Yoshihiro Okabe Junna Saito Mina Katayama Masato Shindo Yusuke Yamada Koichiro Shimomura Kaori Yoneyama Kohki Akiyama Koh Aoki Tohru Ariizumi Hiroshi Ezura Shinjiro Yamaguchi Mikihisa Umehara Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8 International Journal of Molecular Sciences carotenoid cleavage dioxygenase 8 Orobanche minor Phelipanche aegyptiaca shoot branching Solanum lycopersicum strigolactones |
| author_facet |
Shoko Hasegawa Takuya Tsutsumi Shunsuke Fukushima Yoshihiro Okabe Junna Saito Mina Katayama Masato Shindo Yusuke Yamada Koichiro Shimomura Kaori Yoneyama Kohki Akiyama Koh Aoki Tohru Ariizumi Hiroshi Ezura Shinjiro Yamaguchi Mikihisa Umehara |
| author_sort |
Shoko Hasegawa |
| title |
Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8 |
| title_short |
Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8 |
| title_full |
Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8 |
| title_fullStr |
Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8 |
| title_full_unstemmed |
Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8 |
| title_sort |
low infection of phelipanche aegyptiaca in micro-tom mutants deficient in carotenoid cleavage dioxygenase 8 |
| publisher |
MDPI AG |
| series |
International Journal of Molecular Sciences |
| issn |
1422-0067 |
| publishDate |
2018-09-01 |
| description |
Strigolactones (SLs), a group of plant hormones, induce germination of root-parasitic plants and inhibit shoot branching in many plants. Shoot branching is an important trait that affects the number and quality of flowers and fruits. Root-parasitic plants, such as Phelipanche spp., infect tomato roots and cause economic damage in Europe and North Africa—hence why resistant tomato cultivars are needed. In this study, we found carotenoid cleavage dioxygenase 8-defective mutants of Micro-Tom tomato (slccd8) by the “targeting induced local lesions in genomes” (TILLING) method. The mutants showed excess branching, which was suppressed by exogenously applied SL. Grafting shoot scions of the slccd8 mutants onto wild-type (WT) rootstocks restored normal branching in the scions. The levels of endogenous orobanchol and solanacol in WT were enough detectable, whereas that in the slccd8 mutants were below the detection limit of quantification analysis. Accordingly, root exudates of the slccd8 mutants hardly stimulated seed germination of root parasitic plants. In addition, SL deficiency did not critically affect the fruit traits of Micro-Tom. Using a rhizotron system, we also found that Phelipanche aegyptiaca infection was lower in the slccd8 mutants than in wild-type Micro-Tom because of the low germination. We propose that the slccd8 mutants might be useful as new tomato lines resistant to P. aegyptiaca. |
| topic |
carotenoid cleavage dioxygenase 8 Orobanche minor Phelipanche aegyptiaca shoot branching Solanum lycopersicum strigolactones |
| url |
http://www.mdpi.com/1422-0067/19/9/2645 |
| _version_ |
1612612024354734080 |