Comparative secretome analysis of Rhizoctonia solani isolates with different host ranges reveals unique secretomes and cell death inducing effectors

Abstract Rhizoctonia solani is a fungal pathogen causing substantial damage to many of the worlds’ largest food crops including wheat, rice, maize and soybean. Despite impacting global food security, little is known about the pathogenicity mechanisms employed by R. solani. To enable prediction of ef...

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Main Authors:	Jonathan P. Anderson, Jana Sperschneider, Joe Win, Brendan Kidd, Kentaro Yoshida, James Hane, Diane G. O. Saunders, Karam B. Singh
Format:	Article
Language:	English
Published:	Nature Publishing Group 2017-09-01
Series:	Scientific Reports
Online Access:	http://link.springer.com/article/10.1038/s41598-017-10405-y

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spelling	doaj-art-9ef0528c86a94e4f89f64361c9eeb7df2018-09-09T11:51:58ZengNature Publishing GroupScientific Reports2045-23222017-09-017111310.1038/s41598-017-10405-yComparative secretome analysis of Rhizoctonia solani isolates with different host ranges reveals unique secretomes and cell death inducing effectorsJonathan P. Anderson0Jana Sperschneider1Joe Win2Brendan Kidd3Kentaro Yoshida4James Hane5Diane G. O. Saunders6Karam B. Singh7CSIRO Agriculture and FoodCSIRO Agriculture and FoodThe Sainsbury LaboratoryCSIRO Agriculture and FoodThe Sainsbury LaboratoryCSIRO Agriculture and FoodThe Sainsbury LaboratoryCSIRO Agriculture and FoodAbstract Rhizoctonia solani is a fungal pathogen causing substantial damage to many of the worlds’ largest food crops including wheat, rice, maize and soybean. Despite impacting global food security, little is known about the pathogenicity mechanisms employed by R. solani. To enable prediction of effectors possessing either broad efficacy or host specificity, a combined secretome was constructed from a monocot specific isolate, a dicot specific isolate and broad host range isolate infecting both monocot and dicot hosts. Secretome analysis suggested R. solani employs largely different virulence mechanisms to well-studied pathogens, despite in many instances infecting the same host plants. Furthermore, the secretome of the broad host range AG8 isolate may be shaped by maintaining functions for saprophytic life stages while minimising opportunities for host plant recognition. Analysis of possible co-evolution with host plants and in-planta up-regulation in particular, aided identification of effectors including xylanase and inhibitor I9 domain containing proteins able to induce cell death in-planta. The inhibitor I9 domain was more abundant in the secretomes of a wide range of necrotising fungi relative to biotrophs. These findings provide novel targets for further dissection of the virulence mechanisms and potential avenues to control this under-characterised but important pathogen.http://link.springer.com/article/10.1038/s41598-017-10405-y
institution	Open Data Bank
collection	Open Access Journals
building	Directory of Open Access Journals
language	English
format	Article
author	Jonathan P. Anderson Jana Sperschneider Joe Win Brendan Kidd Kentaro Yoshida James Hane Diane G. O. Saunders Karam B. Singh
spellingShingle	Jonathan P. Anderson Jana Sperschneider Joe Win Brendan Kidd Kentaro Yoshida James Hane Diane G. O. Saunders Karam B. Singh Comparative secretome analysis of Rhizoctonia solani isolates with different host ranges reveals unique secretomes and cell death inducing effectors Scientific Reports
author_facet	Jonathan P. Anderson Jana Sperschneider Joe Win Brendan Kidd Kentaro Yoshida James Hane Diane G. O. Saunders Karam B. Singh
author_sort	Jonathan P. Anderson
title	Comparative secretome analysis of Rhizoctonia solani isolates with different host ranges reveals unique secretomes and cell death inducing effectors
title_short	Comparative secretome analysis of Rhizoctonia solani isolates with different host ranges reveals unique secretomes and cell death inducing effectors
title_full	Comparative secretome analysis of Rhizoctonia solani isolates with different host ranges reveals unique secretomes and cell death inducing effectors
title_fullStr	Comparative secretome analysis of Rhizoctonia solani isolates with different host ranges reveals unique secretomes and cell death inducing effectors
title_full_unstemmed	Comparative secretome analysis of Rhizoctonia solani isolates with different host ranges reveals unique secretomes and cell death inducing effectors
title_sort	comparative secretome analysis of rhizoctonia solani isolates with different host ranges reveals unique secretomes and cell death inducing effectors
publisher	Nature Publishing Group
series	Scientific Reports
issn	2045-2322
publishDate	2017-09-01
description	Abstract Rhizoctonia solani is a fungal pathogen causing substantial damage to many of the worlds’ largest food crops including wheat, rice, maize and soybean. Despite impacting global food security, little is known about the pathogenicity mechanisms employed by R. solani. To enable prediction of effectors possessing either broad efficacy or host specificity, a combined secretome was constructed from a monocot specific isolate, a dicot specific isolate and broad host range isolate infecting both monocot and dicot hosts. Secretome analysis suggested R. solani employs largely different virulence mechanisms to well-studied pathogens, despite in many instances infecting the same host plants. Furthermore, the secretome of the broad host range AG8 isolate may be shaped by maintaining functions for saprophytic life stages while minimising opportunities for host plant recognition. Analysis of possible co-evolution with host plants and in-planta up-regulation in particular, aided identification of effectors including xylanase and inhibitor I9 domain containing proteins able to induce cell death in-planta. The inhibitor I9 domain was more abundant in the secretomes of a wide range of necrotising fungi relative to biotrophs. These findings provide novel targets for further dissection of the virulence mechanisms and potential avenues to control this under-characterised but important pathogen.
url	http://link.springer.com/article/10.1038/s41598-017-10405-y
_version_	1612609174932291584

Comparative secretome analysis of Rhizoctonia solani isolates with different host ranges reveals unique secretomes and cell death inducing effectors

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