Transplanted Human Bone Marrow Mesenchymal Stem Cells Seeded onto Peptide Hydrogel Decrease Alveolar Bone Loss

Transplanted Human Bone Marrow Mesenchymal Stem Cells Seeded onto Peptide Hydrogel Decrease Alveolar Bone Loss

Alveolar bone loss can be caused by periodontitis or periodontal trauma. We have evaluated the effects of transplanted undifferentiated human mesenchymal stem cells (hMSCs) on alveolar bone reaction and periodontal ligament healing in an experimental periodontal wound model. The hMSCs seeded onto a...

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Main Authors: Ion Tcacencu, Erik Karlström, Jessica Cedervall, Mikael Wendel
Format: Article
Language:English
Published: Mary Ann Liebert, Inc. publishers 2012-10-01
Series:BioResearch Open Access
Subjects:
bone repair
inflammation
osteoclast
PuraMatrix
Online Access:https://www.liebertpub.com/doi/full/10.1089/BIORES.2012.0239
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recordtype oai_dc
spelling doaj-art-934925cc5b8642178f20e453a5497b342018-08-29T20:32:43ZengMary Ann Liebert, Inc. publishersBioResearch Open Access2164-78602012-10-011521522110.1089/BIORES.2012.0239Transplanted Human Bone Marrow Mesenchymal Stem Cells Seeded onto Peptide Hydrogel Decrease Alveolar Bone LossIon Tcacencu0Erik Karlström1Jessica Cedervall2Mikael Wendel3Department of Dental Medicine, Karolinska InstitutetDepartment of Dental Medicine, Karolinska InstitutetDepartment of Medical Biochemistry and Microbiology, Uppsala UniversityDepartment of Dental Medicine, Karolinska InstitutetAlveolar bone loss can be caused by periodontitis or periodontal trauma. We have evaluated the effects of transplanted undifferentiated human mesenchymal stem cells (hMSCs) on alveolar bone reaction and periodontal ligament healing in an experimental periodontal wound model. The hMSCs seeded onto a self-assembling peptide hydrogel in combination with collagen sponge were implanted into the right mandible of 12 rats and followed for 1 (n=6) or 4 weeks (n=6) postoperatively. The other 12 sham-treated rats were used as controls. Histological and histomorphometrical methods were used to assess the periodontal tissue reaction. The alveolar bone volume density was significantly higher at 1 week after surgery, and the osteoclast number was significantly lower at both 1 week and 4 weeks postoperatively in the mandibles treated with hMSCs. The implanted cells were detected only at 1 week after surgery. In conclusion, transplanted hMSCs can contribute to alveolar bone preservation after a periodontal surgical trauma at least by decreasing local osteoclast number.https://www.liebertpub.com/doi/full/10.1089/BIORES.2012.0239bone repairinflammationosteoclastPuraMatrix
institution Open Data Bank
collection Open Access Journals
building Directory of Open Access Journals
language English
format Article
author Ion Tcacencu
Erik Karlström
Jessica Cedervall
Mikael Wendel
spellingShingle Ion Tcacencu
Erik Karlström
Jessica Cedervall
Mikael Wendel
Transplanted Human Bone Marrow Mesenchymal Stem Cells Seeded onto Peptide Hydrogel Decrease Alveolar Bone Loss
BioResearch Open Access
bone repair
inflammation
osteoclast
PuraMatrix
author_facet Ion Tcacencu
Erik Karlström
Jessica Cedervall
Mikael Wendel
author_sort Ion Tcacencu
title Transplanted Human Bone Marrow Mesenchymal Stem Cells Seeded onto Peptide Hydrogel Decrease Alveolar Bone Loss
title_short Transplanted Human Bone Marrow Mesenchymal Stem Cells Seeded onto Peptide Hydrogel Decrease Alveolar Bone Loss
title_full Transplanted Human Bone Marrow Mesenchymal Stem Cells Seeded onto Peptide Hydrogel Decrease Alveolar Bone Loss
title_fullStr Transplanted Human Bone Marrow Mesenchymal Stem Cells Seeded onto Peptide Hydrogel Decrease Alveolar Bone Loss
title_full_unstemmed Transplanted Human Bone Marrow Mesenchymal Stem Cells Seeded onto Peptide Hydrogel Decrease Alveolar Bone Loss
title_sort transplanted human bone marrow mesenchymal stem cells seeded onto peptide hydrogel decrease alveolar bone loss
publisher Mary Ann Liebert, Inc. publishers
series BioResearch Open Access
issn 2164-7860
publishDate 2012-10-01
description Alveolar bone loss can be caused by periodontitis or periodontal trauma. We have evaluated the effects of transplanted undifferentiated human mesenchymal stem cells (hMSCs) on alveolar bone reaction and periodontal ligament healing in an experimental periodontal wound model. The hMSCs seeded onto a self-assembling peptide hydrogel in combination with collagen sponge were implanted into the right mandible of 12 rats and followed for 1 (n=6) or 4 weeks (n=6) postoperatively. The other 12 sham-treated rats were used as controls. Histological and histomorphometrical methods were used to assess the periodontal tissue reaction. The alveolar bone volume density was significantly higher at 1 week after surgery, and the osteoclast number was significantly lower at both 1 week and 4 weeks postoperatively in the mandibles treated with hMSCs. The implanted cells were detected only at 1 week after surgery. In conclusion, transplanted hMSCs can contribute to alveolar bone preservation after a periodontal surgical trauma at least by decreasing local osteoclast number.
topic bone repair
inflammation
osteoclast
PuraMatrix
url https://www.liebertpub.com/doi/full/10.1089/BIORES.2012.0239
_version_ 1612665928206516224

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