Morphometric and morphological appraisal of the eggs of Anopheles stephensi (Diptera: Culicidae) from India

Background & objectives: Anopheles stephensi is one of the most important urban malaria vectors in India and contribute about 12% of total malaria cases. An. stephensi has three ecological variants; type, intermediate and mysorensis that can be differentiated on the basis of differences in numbe...

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Main Authors:	Varun Tyagi, Sunil Dhiman, Ajay K Sharma, A R Srivastava, Bipul Rabha, D Sukumaran, Vijay Veer
Format:	Article
Language:	English
Published:	Wolters Kluwer Medknow Publications 2017-01-01
Series:	Journal of Vector Borne Diseases
Subjects:	Anopheles stephensi; ecological variants; morphology; morphometric; scanning electron microscopy
Online Access:	http://www.jvbd.org/article.asp?issn=0972-9062;year=2017;volume=54;issue=2;spage=151;epage=156;aulast=Tyagi

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spelling	doaj-art-7ae37ad8e6124e17976d92116d063f052018-09-02T02:22:17ZengWolters Kluwer Medknow PublicationsJournal of Vector Borne Diseases0972-90622017-01-01542151156Morphometric and morphological appraisal of the eggs of Anopheles stephensi (Diptera: Culicidae) from IndiaVarun TyagiSunil DhimanAjay K SharmaA R SrivastavaBipul RabhaD SukumaranVijay VeerBackground & objectives: Anopheles stephensi is one of the most important urban malaria vectors in India and contribute about 12% of total malaria cases. An. stephensi has three ecological variants; type, intermediate and mysorensis that can be differentiated on the basis of differences in number of ridges on egg float and on the basis of spiracular indices. Because of its anthropophilic nature the ′type′ form is an efficient malaria vector. In the present study, the egg surface morphometry and morphology of An. stephensi ′type′ form was studied and detail distinguish- ing characters were recorded for its correct identification. Methods: Eggs of An. stephensi ′type′ form were studied by scanning electron microscopy (SEM) after sputter- coating with gold. In total 23 egg characters were analysed morphologically and morphometrically, which included egg attributes, deck attributes, ventral tubercles, micropyle and float attributes. Results: The dorsal surface of the egg of ′type′ form was curved while the ventral surface was concave and both anterior and posterior ends were blunt. The average length and width of egg was 473.94 + 11.18 and 154.69 + 2.66 μm respectively. The number of float ribs observed was 20.33 ± 0.33. The maximum length of float was found to be 246.57 + 15.27 μm, whereas maximum width was 87.16 + 3.83 μm. Interpretation & conclusion: The present study has generated some important data which is specific to An. Stephensi ′type′ form and provided significant morphological and morphometric standards for its correct identification. This information could be useful in differentiation of An. stephensi ′type′ form from other ecological forms of the same species as well as other species of Anopheles.http://www.jvbd.org/article.asp?issn=0972-9062;year=2017;volume=54;issue=2;spage=151;epage=156;aulast=TyagiAnopheles stephensi; ecological variants; morphology; morphometric; scanning electron microscopy
institution	Open Data Bank
collection	Open Access Journals
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language	English
format	Article
author	Varun Tyagi Sunil Dhiman Ajay K Sharma A R Srivastava Bipul Rabha D Sukumaran Vijay Veer
spellingShingle	Varun Tyagi Sunil Dhiman Ajay K Sharma A R Srivastava Bipul Rabha D Sukumaran Vijay Veer Morphometric and morphological appraisal of the eggs of Anopheles stephensi (Diptera: Culicidae) from India Journal of Vector Borne Diseases Anopheles stephensi; ecological variants; morphology; morphometric; scanning electron microscopy
author_facet	Varun Tyagi Sunil Dhiman Ajay K Sharma A R Srivastava Bipul Rabha D Sukumaran Vijay Veer
author_sort	Varun Tyagi
title	Morphometric and morphological appraisal of the eggs of Anopheles stephensi (Diptera: Culicidae) from India
title_short	Morphometric and morphological appraisal of the eggs of Anopheles stephensi (Diptera: Culicidae) from India
title_full	Morphometric and morphological appraisal of the eggs of Anopheles stephensi (Diptera: Culicidae) from India
title_fullStr	Morphometric and morphological appraisal of the eggs of Anopheles stephensi (Diptera: Culicidae) from India
title_full_unstemmed	Morphometric and morphological appraisal of the eggs of Anopheles stephensi (Diptera: Culicidae) from India
title_sort	morphometric and morphological appraisal of the eggs of anopheles stephensi (diptera: culicidae) from india
publisher	Wolters Kluwer Medknow Publications
series	Journal of Vector Borne Diseases
issn	0972-9062
publishDate	2017-01-01
description	Background & objectives: Anopheles stephensi is one of the most important urban malaria vectors in India and contribute about 12% of total malaria cases. An. stephensi has three ecological variants; type, intermediate and mysorensis that can be differentiated on the basis of differences in number of ridges on egg float and on the basis of spiracular indices. Because of its anthropophilic nature the ′type′ form is an efficient malaria vector. In the present study, the egg surface morphometry and morphology of An. stephensi ′type′ form was studied and detail distinguish- ing characters were recorded for its correct identification. Methods: Eggs of An. stephensi ′type′ form were studied by scanning electron microscopy (SEM) after sputter- coating with gold. In total 23 egg characters were analysed morphologically and morphometrically, which included egg attributes, deck attributes, ventral tubercles, micropyle and float attributes. Results: The dorsal surface of the egg of ′type′ form was curved while the ventral surface was concave and both anterior and posterior ends were blunt. The average length and width of egg was 473.94 + 11.18 and 154.69 + 2.66 μm respectively. The number of float ribs observed was 20.33 ± 0.33. The maximum length of float was found to be 246.57 + 15.27 μm, whereas maximum width was 87.16 + 3.83 μm. Interpretation & conclusion: The present study has generated some important data which is specific to An. Stephensi ′type′ form and provided significant morphological and morphometric standards for its correct identification. This information could be useful in differentiation of An. stephensi ′type′ form from other ecological forms of the same species as well as other species of Anopheles.
topic	Anopheles stephensi; ecological variants; morphology; morphometric; scanning electron microscopy
url	http://www.jvbd.org/article.asp?issn=0972-9062;year=2017;volume=54;issue=2;spage=151;epage=156;aulast=Tyagi
_version_	1612659910455066624

Morphometric and morphological appraisal of the eggs of Anopheles stephensi (Diptera: Culicidae) from India

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