Bacterial vaginosis, human papilloma virus and herpes viridae do not predict vaginal HIV RNA shedding in women living with HIV in Denmark

Abstract Background Bacterial vaginosis (BV) has been found to be associated with HIV acquisition and transmission. This is suggested to be due to higher HIV RNA levels in cervicovaginal fluids in women living with HIV (WLWH) with BV, as bacteria associated with BV may induce viral replication and s...

Full description

Bibliographic Details
Main Authors:	Maria Wessman, Kristina Thorsteinsson, Jørgen S. Jensen, Merete Storgaard, Frederikke F. Rönsholt, Isik S. Johansen, Gitte Pedersen, Lars Nørregård Nielsen, Jesper Bonde, Terese L. Katzenstein, Nina Weis, Anne-Mette Lebech
Format:	Article
Language:	English
Published:	BioMed Central 2017-05-01
Series:	BMC Infectious Diseases
Subjects:	Women living with HIV Bacterial vaginosis HIV RNA vaginal shedding Herpes viridae Human papilloma virus
Online Access:	http://link.springer.com/article/10.1186/s12879-017-2477-7

id	doaj-art-727fa328edb74fe48f298bd21888b211
recordtype	oai_dc
spelling	doaj-art-727fa328edb74fe48f298bd21888b2112018-08-15T22:19:58ZengBioMed CentralBMC Infectious Diseases1471-23342017-05-0117111010.1186/s12879-017-2477-7Bacterial vaginosis, human papilloma virus and herpes viridae do not predict vaginal HIV RNA shedding in women living with HIV in DenmarkMaria Wessman0Kristina Thorsteinsson1Jørgen S. Jensen2Merete Storgaard3Frederikke F. Rönsholt4Isik S. Johansen5Gitte Pedersen6Lars Nørregård Nielsen7Jesper Bonde8Terese L. Katzenstein9Nina Weis10Anne-Mette Lebech11Department of Infectious Diseases, Copenhagen University HospitalDepartment of Infectious Diseases, Copenhagen University HospitalDepartment of Microbiology and Infection Control, Statens Serum InstitutDepartment of Infectious Diseases, Aarhus University HospitalDepartment of Infectious Diseases, Copenhagen University HospitalDepartment of Infectious Diseases, Odense University HospitalDepartment of Infectious Diseases, Aalborg University HospitalDepartment of Lung- and Infectious Diseases, Nordsjællands HospitalDepartment of Pathology, Copenhagen University HospitalDepartment of Infectious Diseases, Copenhagen University HospitalDepartment of Infectious Diseases, Copenhagen University HospitalDepartment of Infectious Diseases, Copenhagen University HospitalAbstract Background Bacterial vaginosis (BV) has been found to be associated with HIV acquisition and transmission. This is suggested to be due to higher HIV RNA levels in cervicovaginal fluids in women living with HIV (WLWH) with BV, as bacteria associated with BV may induce viral replication and shedding in the genital tract despite undetectable HIV RNA plasma viral load. We examined the prevalence and diagnostic predictors of BV and HIV-1 RNA vaginal shedding in women living with HIV (WLWH) in Denmark, taking into account the presence of human papillomavirus (HPV) and herpes viridae. Methods WLWH between 18–51 years were recruited from six Departments of Infectious Diseases in Denmark during enrolment in the SHADE cohort; a prospective cohort study of WLWH attending regular outpatient care. BV was diagnosed by microscopy of vaginal swabs and PCR was used for detection of BV-associated bacteria, HPV, herpes viridae, and vaginal HIV viral load. Results Median age of the 150 included women was 41 years; ethnicity was predominantly White (35%) or Black (47%). The majority (96%) was on ART and had undetectable (85%) plasma HIV RNA (<40 copies/mL). BV was diagnosed in 32%. Overall, 11% had detectable vaginal HIV RNA. Both before and after adjustment for BV, age, ethnicity, plasma HIV RNA, CD4 cell count, herpes viridae and HPV, we found no significant predictors of HIV RNA vaginal shedding. Conclusion In well-treated WLWH, BV, herpes viridae or HPV do not predict vaginal HIV RNA shedding. This implies that HIV shedding does not seem to be increased by BV.http://link.springer.com/article/10.1186/s12879-017-2477-7Women living with HIVBacterial vaginosisHIV RNA vaginal sheddingHerpes viridaeHuman papilloma virus
institution	Open Data Bank
collection	Open Access Journals
building	Directory of Open Access Journals
language	English
format	Article
author	Maria Wessman Kristina Thorsteinsson Jørgen S. Jensen Merete Storgaard Frederikke F. Rönsholt Isik S. Johansen Gitte Pedersen Lars Nørregård Nielsen Jesper Bonde Terese L. Katzenstein Nina Weis Anne-Mette Lebech
spellingShingle	Maria Wessman Kristina Thorsteinsson Jørgen S. Jensen Merete Storgaard Frederikke F. Rönsholt Isik S. Johansen Gitte Pedersen Lars Nørregård Nielsen Jesper Bonde Terese L. Katzenstein Nina Weis Anne-Mette Lebech Bacterial vaginosis, human papilloma virus and herpes viridae do not predict vaginal HIV RNA shedding in women living with HIV in Denmark BMC Infectious Diseases Women living with HIV Bacterial vaginosis HIV RNA vaginal shedding Herpes viridae Human papilloma virus
author_facet	Maria Wessman Kristina Thorsteinsson Jørgen S. Jensen Merete Storgaard Frederikke F. Rönsholt Isik S. Johansen Gitte Pedersen Lars Nørregård Nielsen Jesper Bonde Terese L. Katzenstein Nina Weis Anne-Mette Lebech
author_sort	Maria Wessman
title	Bacterial vaginosis, human papilloma virus and herpes viridae do not predict vaginal HIV RNA shedding in women living with HIV in Denmark
title_short	Bacterial vaginosis, human papilloma virus and herpes viridae do not predict vaginal HIV RNA shedding in women living with HIV in Denmark
title_full	Bacterial vaginosis, human papilloma virus and herpes viridae do not predict vaginal HIV RNA shedding in women living with HIV in Denmark
title_fullStr	Bacterial vaginosis, human papilloma virus and herpes viridae do not predict vaginal HIV RNA shedding in women living with HIV in Denmark
title_full_unstemmed	Bacterial vaginosis, human papilloma virus and herpes viridae do not predict vaginal HIV RNA shedding in women living with HIV in Denmark
title_sort	bacterial vaginosis, human papilloma virus and herpes viridae do not predict vaginal hiv rna shedding in women living with hiv in denmark
publisher	BioMed Central
series	BMC Infectious Diseases
issn	1471-2334
publishDate	2017-05-01
description	Abstract Background Bacterial vaginosis (BV) has been found to be associated with HIV acquisition and transmission. This is suggested to be due to higher HIV RNA levels in cervicovaginal fluids in women living with HIV (WLWH) with BV, as bacteria associated with BV may induce viral replication and shedding in the genital tract despite undetectable HIV RNA plasma viral load. We examined the prevalence and diagnostic predictors of BV and HIV-1 RNA vaginal shedding in women living with HIV (WLWH) in Denmark, taking into account the presence of human papillomavirus (HPV) and herpes viridae. Methods WLWH between 18–51 years were recruited from six Departments of Infectious Diseases in Denmark during enrolment in the SHADE cohort; a prospective cohort study of WLWH attending regular outpatient care. BV was diagnosed by microscopy of vaginal swabs and PCR was used for detection of BV-associated bacteria, HPV, herpes viridae, and vaginal HIV viral load. Results Median age of the 150 included women was 41 years; ethnicity was predominantly White (35%) or Black (47%). The majority (96%) was on ART and had undetectable (85%) plasma HIV RNA (<40 copies/mL). BV was diagnosed in 32%. Overall, 11% had detectable vaginal HIV RNA. Both before and after adjustment for BV, age, ethnicity, plasma HIV RNA, CD4 cell count, herpes viridae and HPV, we found no significant predictors of HIV RNA vaginal shedding. Conclusion In well-treated WLWH, BV, herpes viridae or HPV do not predict vaginal HIV RNA shedding. This implies that HIV shedding does not seem to be increased by BV.
topic	Women living with HIV Bacterial vaginosis HIV RNA vaginal shedding Herpes viridae Human papilloma virus
url	http://link.springer.com/article/10.1186/s12879-017-2477-7
_version_	1612703966329569280

Bacterial vaginosis, human papilloma virus and herpes viridae do not predict vaginal HIV RNA shedding in women living with HIV in Denmark

Similar Items