OXIDATIVE STRESS IN THE PATIENTS WITH CKD V STAGE AND ANEMIA WHO RECEIVE REPLACEMENT HEMODIALYSIS THARAPY

OXIDATIVE STRESS IN THE PATIENTS WITH CKD V STAGE AND ANEMIA WHO RECEIVE REPLACEMENT HEMODIALYSIS THARAPY

The article presents research data of oxidative stress role in the pathogenesis of anemia in patients with CKD V stage, who receive hemodialysis replacement therapy. Background. Oxidative stress is a part of the pathogenesis of many complications in patients with CKD stage V on replacement therap...

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Main Authors: E. S. Krutikov, T. F. Polishchuk, L. V. Polskaya, A. A. Shakhnazarov
Format: Article
Language:English
Published: State Institution «Institute of Nephrology NAMS of Ukraine" 2017-12-01
Series:Український Журнал Нефрології та Діалізу
Subjects:
chronic kidney disease, dialysis, anemia, oxidative stress
Online Access:https://ukrjnd.com.ua/index.php/journal/article/view/105
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spelling doaj-art-5722302d14804ef5bb7466f62dabefdc2018-09-03T10:42:49ZengState Institution «Institute of Nephrology NAMS of Ukraine" Український Журнал Нефрології та Діалізу2304-02382616-73522017-12-011(37)212510.31450/ukrjnd.1(37).2013.04105OXIDATIVE STRESS IN THE PATIENTS WITH CKD V STAGE AND ANEMIA WHO RECEIVE REPLACEMENT HEMODIALYSIS THARAPYE. S. KrutikovT. F. PolishchukL. V. PolskayaA. A. ShakhnazarovThe article presents research data of oxidative stress role in the pathogenesis of anemia in patients with CKD V stage, who receive hemodialysis replacement therapy. Background. Oxidative stress is a part of the pathogenesis of many complications in patients with CKD stage V on replacement therapy with hemodialysis. Anemia syndrome is the most frequent complication of this patient`s population. Aim. The aim of this study was to study indicators of oxidative stress in patients with            CKD stage V and anemia on replacement therapy with hemodialysis. Materials and methods. We studied laboratory parameters in 42 hemodialysis patients with CKD V stage. Biochemical methods are used for investigation of lipid peroxidation processes: studied diene conjugates (DC), malondialdehyde (MDA), total lipids and catalase, lipid’s structure of erythrocytes membranes and their osmotic resistance. Results. We found that in dialysis patients anemic syndrome was associated with high activity of oxidative stress. Also increased destruction of red blood cells depends from high activity of oxidative stress. In the blood of the patients concentration of DC was 1,12±0,26 opt.den.units/ mg of lipids (p<0,01), and MDA level 0,35±0,05 opt.den.units/ mg of lipids (p<0,01). Levels of catalase, decreased to 0,029±0,0019 mm/l*sec (p<0,001). Also osmotic resistance of erythrocytes significantly decreased. MDA has inverse correlation relationship with hemoglobin in the blood, with a correlation coefficient r=-0,36. Investigation of erythrocyte membrane lipid structure of patients by TLC showed a significant decrease of phospholipids and monoglycerides of higher fatty acids, and the increase of cholesterol fractions. Conclusions. In patients with chronic kidney disease on dialysis replacement therapy anemic syndrome is associated with high activity of oxidative stress, as there is an inverse relationship between the level of hemoglobin and the content of MDA in blood. As a consequence of oxidative stress changes the lipid composition of erythrocyte membranes of the patient, because some fraction of lipids are substrates for oxidative processes. Impairment of lipid membranes of red blood cells of hemodialysis patients is one of the probably causes of anemia.https://ukrjnd.com.ua/index.php/journal/article/view/105chronic kidney disease, dialysis, anemia, oxidative stress
institution Open Data Bank
collection Open Access Journals
building Directory of Open Access Journals
language English
format Article
author E. S. Krutikov
T. F. Polishchuk
L. V. Polskaya
A. A. Shakhnazarov
spellingShingle E. S. Krutikov
T. F. Polishchuk
L. V. Polskaya
A. A. Shakhnazarov
OXIDATIVE STRESS IN THE PATIENTS WITH CKD V STAGE AND ANEMIA WHO RECEIVE REPLACEMENT HEMODIALYSIS THARAPY
Український Журнал Нефрології та Діалізу
chronic kidney disease, dialysis, anemia, oxidative stress
author_facet E. S. Krutikov
T. F. Polishchuk
L. V. Polskaya
A. A. Shakhnazarov
author_sort E. S. Krutikov
title OXIDATIVE STRESS IN THE PATIENTS WITH CKD V STAGE AND ANEMIA WHO RECEIVE REPLACEMENT HEMODIALYSIS THARAPY
title_short OXIDATIVE STRESS IN THE PATIENTS WITH CKD V STAGE AND ANEMIA WHO RECEIVE REPLACEMENT HEMODIALYSIS THARAPY
title_full OXIDATIVE STRESS IN THE PATIENTS WITH CKD V STAGE AND ANEMIA WHO RECEIVE REPLACEMENT HEMODIALYSIS THARAPY
title_fullStr OXIDATIVE STRESS IN THE PATIENTS WITH CKD V STAGE AND ANEMIA WHO RECEIVE REPLACEMENT HEMODIALYSIS THARAPY
title_full_unstemmed OXIDATIVE STRESS IN THE PATIENTS WITH CKD V STAGE AND ANEMIA WHO RECEIVE REPLACEMENT HEMODIALYSIS THARAPY
title_sort oxidative stress in the patients with ckd v stage and anemia who receive replacement hemodialysis tharapy
publisher State Institution «Institute of Nephrology NAMS of Ukraine"
series Український Журнал Нефрології та Діалізу
issn 2304-0238
2616-7352
publishDate 2017-12-01
description The article presents research data of oxidative stress role in the pathogenesis of anemia in patients with CKD V stage, who receive hemodialysis replacement therapy. Background. Oxidative stress is a part of the pathogenesis of many complications in patients with CKD stage V on replacement therapy with hemodialysis. Anemia syndrome is the most frequent complication of this patient`s population. Aim. The aim of this study was to study indicators of oxidative stress in patients with            CKD stage V and anemia on replacement therapy with hemodialysis. Materials and methods. We studied laboratory parameters in 42 hemodialysis patients with CKD V stage. Biochemical methods are used for investigation of lipid peroxidation processes: studied diene conjugates (DC), malondialdehyde (MDA), total lipids and catalase, lipid’s structure of erythrocytes membranes and their osmotic resistance. Results. We found that in dialysis patients anemic syndrome was associated with high activity of oxidative stress. Also increased destruction of red blood cells depends from high activity of oxidative stress. In the blood of the patients concentration of DC was 1,12±0,26 opt.den.units/ mg of lipids (p<0,01), and MDA level 0,35±0,05 opt.den.units/ mg of lipids (p<0,01). Levels of catalase, decreased to 0,029±0,0019 mm/l*sec (p<0,001). Also osmotic resistance of erythrocytes significantly decreased. MDA has inverse correlation relationship with hemoglobin in the blood, with a correlation coefficient r=-0,36. Investigation of erythrocyte membrane lipid structure of patients by TLC showed a significant decrease of phospholipids and monoglycerides of higher fatty acids, and the increase of cholesterol fractions. Conclusions. In patients with chronic kidney disease on dialysis replacement therapy anemic syndrome is associated with high activity of oxidative stress, as there is an inverse relationship between the level of hemoglobin and the content of MDA in blood. As a consequence of oxidative stress changes the lipid composition of erythrocyte membranes of the patient, because some fraction of lipids are substrates for oxidative processes. Impairment of lipid membranes of red blood cells of hemodialysis patients is one of the probably causes of anemia.
topic chronic kidney disease, dialysis, anemia, oxidative stress
url https://ukrjnd.com.ua/index.php/journal/article/view/105
_version_ 1612620398107557888

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