Complete sequence-based pathway analysis by differential on-chip DNA and RNA extraction from a single cell

Complete sequence-based pathway analysis by differential on-chip DNA and RNA extraction from a single cell

Abstract We demonstrate on-chip, differential DNA and RNA extraction from a single cell using a microfluidic chip and a two-stage lysis protocol. This method enables direct use of the whole extract, without additional washing steps, reducing sample loss. Using this method, the tumor driving pathway...

Full description

Bibliographic Details
Main Authors: D. van Strijp, R. C. M. Vulders, N. A. Larsen, J. Schira, L. Baerlocher, M. A. van Driel, M. Pødenphant, T. S. Hansen, A. Kristensen, K. U. Mir, T. Olesen, W. F. J. Verhaegh, R. Marie, P. J. van der Zaag
Format: Article
Language:English
Published: Nature Publishing Group 2017-09-01
Series:Scientific Reports
Online Access:http://link.springer.com/article/10.1038/s41598-017-10704-4

Internet

http://link.springer.com/article/10.1038/s41598-017-10704-4

Similar Items