Phytochemical, antioxidant and hepatoprotective effects of different fractions of Moringa oleifera leaves methanol extract against liver injury in animal model
Objective: To evaluate the potential antioxidant and hepatoprotective effects of n-hexane, dichloromethane(DCM), ethyl acetate(EtOAc), n-butanol and aqueous fractions of Moringa oleifera(M. oleifera) leaves methanol extract against carbon tetrachloride(CCl4)-induced liver injury in rats. Methods: Th...
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Wolters Kluwer Medknow Publications
2018-01-01
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doaj-art-51cd0ee603734bd7b18f1f3a5987f1a72018-09-06T10:57:25ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Medicine2352-41462018-01-0111742342910.4103/1995-7645.237186Phytochemical, antioxidant and hepatoprotective effects of different fractions of Moringa oleifera leaves methanol extract against liver injury in animal modelAttia H AttaSoad M NasrAbduljalil H AlmaweriDoaa SedkyAmany M MohamedHassan M DesoukyMostafa A ShalabyObjective: To evaluate the potential antioxidant and hepatoprotective effects of n-hexane, dichloromethane(DCM), ethyl acetate(EtOAc), n-butanol and aqueous fractions of Moringa oleifera(M. oleifera) leaves methanol extract against carbon tetrachloride(CCl4)-induced liver injury in rats. Methods: These fractions were prepared from the M. oleifera leaves methanol extract by solubilization in water and partitioning in n-hexane, EtOAc, DCM and n-butanol. Their phyto-components were identified by GC-MS analysis. The in vitro antioxidant effect of these fractions was carried out by assessment of 1,1-diphenyl-2-picrylhydrazyl scavenging activity. A total of 40 Sprague Dawley rats were allocated into 8 equal groups: group 1 given olive oil (1 mL/kg b.wt.), group 2 injected with CCl4, group 3 to 7 administered with n-hexane, DCM, EtOAc, n-butanol and aqueous fractions, respectively after CCl4, group 8 administered with silymarin after CCl4. The activities of aspartate aminotransferase, alanine aminotransferase, and the levels of total cholesterol, triglycerides, glucose, total proteins and albumin in serum were determined spectrophotometrically. Glutathione reduced, lipid peroxide by-products levels, glutathione-s-transferase and catalase enzyme activities in the liver homogenate were determined by spectrophotometer. Liver specimens were also examined for histopathological alterations under light microscope. Results: The GC-MS analysis of different fractions of the M. oleifera leaves methanol extract revealed that n-hexane, DCM, EtOAc, n-butanol, and aqueous fractions contained 17, 22, 23, 19 and 32 compounds, respectively. The percent and the molecular structure of each component in each fraction were identified. The n-butanol and EtOAc fractions exhibited the strongest in vitro antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl. CCl4 significantly decreased glutathione reduced and total proteins concentration and glutathione-s-transferase and catalase activities but increased lipid peroxide by-products and total cholesterol levels. The n-hexane followed by aqueous and DCM fractions were the most potent to regulate serum enzyme activities and lipid peroxide by-products levels in the liver homogenate. Conclusions: n-hexane, DCM, and aqueous fractions have the highest effectiveness against CCl4-induced hepatotoxicity. Isolation and purification of the active constituents require further experiments.http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=7;spage=423;epage=429;aulast=AttaHepatoprotectiveAntioxidantsMoringa oleiferaFractionsPhytochemical |
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Attia H Atta Soad M Nasr Abduljalil H Almaweri Doaa Sedky Amany M Mohamed Hassan M Desouky Mostafa A Shalaby |
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Attia H Atta Soad M Nasr Abduljalil H Almaweri Doaa Sedky Amany M Mohamed Hassan M Desouky Mostafa A Shalaby Phytochemical, antioxidant and hepatoprotective effects of different fractions of Moringa oleifera leaves methanol extract against liver injury in animal model Asian Pacific Journal of Tropical Medicine Hepatoprotective Antioxidants Moringa oleifera Fractions Phytochemical |
| author_facet |
Attia H Atta Soad M Nasr Abduljalil H Almaweri Doaa Sedky Amany M Mohamed Hassan M Desouky Mostafa A Shalaby |
| author_sort |
Attia H Atta |
| title |
Phytochemical, antioxidant and hepatoprotective effects of different fractions of Moringa oleifera leaves methanol extract against liver injury in animal model |
| title_short |
Phytochemical, antioxidant and hepatoprotective effects of different fractions of Moringa oleifera leaves methanol extract against liver injury in animal model |
| title_full |
Phytochemical, antioxidant and hepatoprotective effects of different fractions of Moringa oleifera leaves methanol extract against liver injury in animal model |
| title_fullStr |
Phytochemical, antioxidant and hepatoprotective effects of different fractions of Moringa oleifera leaves methanol extract against liver injury in animal model |
| title_full_unstemmed |
Phytochemical, antioxidant and hepatoprotective effects of different fractions of Moringa oleifera leaves methanol extract against liver injury in animal model |
| title_sort |
phytochemical, antioxidant and hepatoprotective effects of different fractions of moringa oleifera leaves methanol extract against liver injury in animal model |
| publisher |
Wolters Kluwer Medknow Publications |
| series |
Asian Pacific Journal of Tropical Medicine |
| issn |
2352-4146 |
| publishDate |
2018-01-01 |
| description |
Objective: To evaluate the potential antioxidant and hepatoprotective effects of n-hexane, dichloromethane(DCM), ethyl acetate(EtOAc), n-butanol and aqueous fractions of Moringa oleifera(M. oleifera) leaves methanol extract against carbon tetrachloride(CCl4)-induced liver injury in rats. Methods: These fractions were prepared from the M. oleifera leaves methanol extract by solubilization in water and partitioning in n-hexane, EtOAc, DCM and n-butanol. Their phyto-components were identified by GC-MS analysis. The in vitro antioxidant effect of these fractions was carried out by assessment of 1,1-diphenyl-2-picrylhydrazyl scavenging activity. A total of 40 Sprague Dawley rats were allocated into 8 equal groups: group 1 given olive oil (1 mL/kg b.wt.), group 2 injected with CCl4, group 3 to 7 administered with n-hexane, DCM, EtOAc, n-butanol and aqueous fractions, respectively after CCl4, group 8 administered with silymarin after CCl4. The activities of aspartate aminotransferase, alanine aminotransferase, and the levels of total cholesterol, triglycerides, glucose, total proteins and albumin in serum were determined spectrophotometrically. Glutathione reduced, lipid peroxide by-products levels, glutathione-s-transferase and catalase enzyme activities in the liver homogenate were determined by spectrophotometer. Liver specimens were also examined for histopathological alterations under light microscope. Results: The GC-MS analysis of different fractions of the M. oleifera leaves methanol extract revealed that n-hexane, DCM, EtOAc, n-butanol, and aqueous fractions contained 17, 22, 23, 19 and 32 compounds, respectively. The percent and the molecular structure of each component in each fraction were identified. The n-butanol and EtOAc fractions exhibited the strongest in vitro antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl. CCl4 significantly decreased glutathione reduced and total proteins concentration and glutathione-s-transferase and catalase activities but increased lipid peroxide by-products and total cholesterol levels. The n-hexane followed by aqueous and DCM fractions were the most potent to regulate serum enzyme activities and lipid peroxide by-products levels in the liver homogenate. Conclusions: n-hexane, DCM, and aqueous fractions have the highest effectiveness against CCl4-induced hepatotoxicity. Isolation and purification of the active constituents require further experiments. |
| topic |
Hepatoprotective Antioxidants Moringa oleifera Fractions Phytochemical |
| url |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=7;spage=423;epage=429;aulast=Atta |
| _version_ |
1612614553841958912 |