The recognition and characterisation of Finnish Clostridium difficile isolates resembling PCR-ribotype 027
Purpose: To characterise and compare twenty-eight Finnish Clostridium difficile RT027-like isolates, selected based on the presence of 18 bp deletion in the tcdC gene and toxin gene profile (A, B, binary), with eleven RT027 isolates from different Finnish geographical areas and time periods. Methods...
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2018-06-01
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| Series: | Journal of Microbiology, Immunology and Infection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1684118217300518 |
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doaj-art-48aa30b2417446fa993818a02d294a292018-09-02T22:14:02ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822018-06-0151334435110.1016/j.jmii.2017.02.002The recognition and characterisation of Finnish Clostridium difficile isolates resembling PCR-ribotype 027Marcela Krutova0Otakar Nyc1Jana Matejkova2Ed J. Kuijper3Jari Jalava4Silja Mentula5Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University in Prague and Motol University Hospital, Czech RepublicDepartment of Medical Microbiology, 2nd Faculty of Medicine, Charles University in Prague and Motol University Hospital, Czech RepublicDepartment of Medical Microbiology, 2nd Faculty of Medicine, Charles University in Prague and Motol University Hospital, Czech RepublicLeiden University Medical Centre, Leiden, The NetherlandsBacterial Infections Unit, Department of Infectious Diseases, National Institute for Health and Welfare, Helsinki, FinlandBacterial Infections Unit, Department of Infectious Diseases, National Institute for Health and Welfare, Helsinki, FinlandPurpose: To characterise and compare twenty-eight Finnish Clostridium difficile RT027-like isolates, selected based on the presence of 18 bp deletion in the tcdC gene and toxin gene profile (A, B, binary), with eleven RT027 isolates from different Finnish geographical areas and time periods. Methods: Twenty-eight C. difficile RT027-like isolates and 11 RT027 comparative strains were characterised by capillary-electrophoresis (CE) ribotyping, multi-locus variable tandem-repeats analysis (MLVA), multi-locus sequence typing (MLST), and sequencing of tcdC and gyrA gene fragments. Susceptibility to moxifloxacin was determined by E-test. Results: Of 28 RT027-like isolates, seven RTs (016, 034, 075, 080, 153, 176 and 328), three WEBRIBO types (411, 475, AI-78) and three new profiles (F1–F3) were identified. MLVA revealed six clonal complexes (RTs 016, 027, 176 and F3). MLST showed eleven sequence types (1, 41, 47, 67, 95, 191,192, 223, 229, 264 and new ST). Twenty-two isolates (RTs 016, 080, 176, 328, F1, F2, F3 and WRTAI-78) carried Δ117 in the tcdC gene. Isolates of RTs 016, 027 and 176 were moxifloxacin resistant and harboured Thr82Ile in the GyrA. Conclusion: Our results show a high diversity within 28 Finnish RT027-like C. difficile isolates, with twelve CE-ribotyping profiles and eleven STs. MLVA revealed the regional spread of RTs 016, 027, 176 and F3. The presence of Δ117 in the tcdC gene in eight non-027 RTs highlights the importance of careful interpretation of the results from molecular systems targeting this site in the genome of C. difficile and the need of strain typing for epidemiological purposes.http://www.sciencedirect.com/science/article/pii/S1684118217300518Capillary-electrophoresis ribotypingClostridium difficileMLSTMLVAMoxifloxacin resistance |
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English |
| format |
Article |
| author |
Marcela Krutova Otakar Nyc Jana Matejkova Ed J. Kuijper Jari Jalava Silja Mentula |
| spellingShingle |
Marcela Krutova Otakar Nyc Jana Matejkova Ed J. Kuijper Jari Jalava Silja Mentula The recognition and characterisation of Finnish Clostridium difficile isolates resembling PCR-ribotype 027 Journal of Microbiology, Immunology and Infection Capillary-electrophoresis ribotyping Clostridium difficile MLST MLVA Moxifloxacin resistance |
| author_facet |
Marcela Krutova Otakar Nyc Jana Matejkova Ed J. Kuijper Jari Jalava Silja Mentula |
| author_sort |
Marcela Krutova |
| title |
The recognition and characterisation of Finnish Clostridium difficile isolates resembling PCR-ribotype 027 |
| title_short |
The recognition and characterisation of Finnish Clostridium difficile isolates resembling PCR-ribotype 027 |
| title_full |
The recognition and characterisation of Finnish Clostridium difficile isolates resembling PCR-ribotype 027 |
| title_fullStr |
The recognition and characterisation of Finnish Clostridium difficile isolates resembling PCR-ribotype 027 |
| title_full_unstemmed |
The recognition and characterisation of Finnish Clostridium difficile isolates resembling PCR-ribotype 027 |
| title_sort |
recognition and characterisation of finnish clostridium difficile isolates resembling pcr-ribotype 027 |
| publisher |
Elsevier |
| series |
Journal of Microbiology, Immunology and Infection |
| issn |
1684-1182 |
| publishDate |
2018-06-01 |
| description |
Purpose: To characterise and compare twenty-eight Finnish Clostridium difficile RT027-like isolates, selected based on the presence of 18 bp deletion in the tcdC gene and toxin gene profile (A, B, binary), with eleven RT027 isolates from different Finnish geographical areas and time periods.
Methods: Twenty-eight C. difficile RT027-like isolates and 11 RT027 comparative strains were characterised by capillary-electrophoresis (CE) ribotyping, multi-locus variable tandem-repeats analysis (MLVA), multi-locus sequence typing (MLST), and sequencing of tcdC and gyrA gene fragments. Susceptibility to moxifloxacin was determined by E-test.
Results: Of 28 RT027-like isolates, seven RTs (016, 034, 075, 080, 153, 176 and 328), three WEBRIBO types (411, 475, AI-78) and three new profiles (F1–F3) were identified. MLVA revealed six clonal complexes (RTs 016, 027, 176 and F3). MLST showed eleven sequence types (1, 41, 47, 67, 95, 191,192, 223, 229, 264 and new ST). Twenty-two isolates (RTs 016, 080, 176, 328, F1, F2, F3 and WRTAI-78) carried Δ117 in the tcdC gene. Isolates of RTs 016, 027 and 176 were moxifloxacin resistant and harboured Thr82Ile in the GyrA.
Conclusion: Our results show a high diversity within 28 Finnish RT027-like C. difficile isolates, with twelve CE-ribotyping profiles and eleven STs. MLVA revealed the regional spread of RTs 016, 027, 176 and F3. The presence of Δ117 in the tcdC gene in eight non-027 RTs highlights the importance of careful interpretation of the results from molecular systems targeting this site in the genome of C. difficile and the need of strain typing for epidemiological purposes. |
| topic |
Capillary-electrophoresis ribotyping Clostridium difficile MLST MLVA Moxifloxacin resistance |
| url |
http://www.sciencedirect.com/science/article/pii/S1684118217300518 |
| _version_ |
1612622484117389312 |