The association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPD

Abstract Background Interleukin(IL)-33 is an epithelial alarmin important for eosinophil maturation, activation and survival. The aim of this study was to examine the association between IL-33, its receptor expression and airway eosinophilic inflammation in non-atopic COPD. Methods IL-33 concentrati...

Full description

Bibliographic Details
Main Authors: Damian Tworek, Sebastian Majewski, Karolina Szewczyk, Justyna Kiszałkiewicz, Zofia Kurmanowska, Paweł Górski, Ewa Brzeziańska-Lasota, Piotr Kuna, Adam Antczak
Format: Article
Language:English
Published: BioMed Central 2018-06-01
Series:Respiratory Research
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12931-018-0807-y
id doaj-art-363279e872e44c93a78ebdf83c224d85
recordtype oai_dc
spelling doaj-art-363279e872e44c93a78ebdf83c224d852018-08-15T23:43:47ZengBioMed CentralRespiratory Research1465-993X2018-06-0119111110.1186/s12931-018-0807-yThe association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPDDamian Tworek0Sebastian Majewski1Karolina Szewczyk2Justyna Kiszałkiewicz3Zofia Kurmanowska4Paweł Górski5Ewa Brzeziańska-Lasota6Piotr Kuna7Adam Antczak8Department of General and Oncological Pulmonology, Medical University of LodzDepartment of Pulmonology and Allergy, Medical University of LodzDepartment of Pulmonology and Allergy, Medical University of LodzDepartment of Biomedicine and Genetics, Medical University of LodzDepartment of Pulmonology and Allergy, Medical University of LodzDepartment of Pulmonology and Allergy, Medical University of LodzDepartment of Biomedicine and Genetics, Medical University of LodzDepartment of Internal Medicine, Asthma and Allergy, Medical University of LodzDepartment of General and Oncological Pulmonology, Medical University of LodzAbstract Background Interleukin(IL)-33 is an epithelial alarmin important for eosinophil maturation, activation and survival. The aim of this study was to examine the association between IL-33, its receptor expression and airway eosinophilic inflammation in non-atopic COPD. Methods IL-33 concentrations were measured in exhaled breath condensate (EBC) collected from healthy non-smokers, asthmatics and non-atopic COPD subjects using ELISA. Serum and sputum samples were collected from healthy non-smokers, healthy smokers and non-atopic COPD patients. Based on sputum eosinophil count, COPD subjects were divided into subgroups with airway eosinophilic inflammation (sputum eosinophils > 3%) or without (sputum eosinophils ≤3%). IL-33 and soluble form of IL-33 receptor (sST2) protein concentrations were measured in serum and sputum supernatants using ELISA. ST2 mRNA expression was measured in peripheral mononuclear cells and sputum cells by qPCR. Hemopoietic progenitor cells (HPC) expressing ST2 and intracellular IL-5 were enumerated in blood and induced sputum by means of flow cytometry. Results IL-33 levels in EBC were increased in COPD patients to a similar extent as in asthma and correlated with blood eosinophil count. Furthermore, serum and sputum IL-33 levels were higher in COPD subjects with sputum eosinophilia than in those with a sputum eosinophil count ≤3% (p < 0.001 for both). ST2 mRNA was overexpressed in sputum cells obtained from COPD patients with airway eosinophilic inflammation compared to those without sputum eosinophilia (p < 0.01). Similarly, ST2 + IL-5+ HPC numbers were increased in the sputum of COPD patients with airway eosinophilia (p < 0.001). Conclusions Our results indicate that IL-33 is involved in the development of eosinophilic airway inflammation in non-atopic COPD patients.http://link.springer.com/article/10.1186/s12931-018-0807-yIL-33EosinophilsCOPD
institution Open Data Bank
collection Open Access Journals
building Directory of Open Access Journals
language English
format Article
author Damian Tworek
Sebastian Majewski
Karolina Szewczyk
Justyna Kiszałkiewicz
Zofia Kurmanowska
Paweł Górski
Ewa Brzeziańska-Lasota
Piotr Kuna
Adam Antczak
spellingShingle Damian Tworek
Sebastian Majewski
Karolina Szewczyk
Justyna Kiszałkiewicz
Zofia Kurmanowska
Paweł Górski
Ewa Brzeziańska-Lasota
Piotr Kuna
Adam Antczak
The association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPD
Respiratory Research
IL-33
Eosinophils
COPD
author_facet Damian Tworek
Sebastian Majewski
Karolina Szewczyk
Justyna Kiszałkiewicz
Zofia Kurmanowska
Paweł Górski
Ewa Brzeziańska-Lasota
Piotr Kuna
Adam Antczak
author_sort Damian Tworek
title The association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPD
title_short The association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPD
title_full The association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPD
title_fullStr The association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPD
title_full_unstemmed The association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPD
title_sort association between airway eosinophilic inflammation and il-33 in stable non-atopic copd
publisher BioMed Central
series Respiratory Research
issn 1465-993X
publishDate 2018-06-01
description Abstract Background Interleukin(IL)-33 is an epithelial alarmin important for eosinophil maturation, activation and survival. The aim of this study was to examine the association between IL-33, its receptor expression and airway eosinophilic inflammation in non-atopic COPD. Methods IL-33 concentrations were measured in exhaled breath condensate (EBC) collected from healthy non-smokers, asthmatics and non-atopic COPD subjects using ELISA. Serum and sputum samples were collected from healthy non-smokers, healthy smokers and non-atopic COPD patients. Based on sputum eosinophil count, COPD subjects were divided into subgroups with airway eosinophilic inflammation (sputum eosinophils > 3%) or without (sputum eosinophils ≤3%). IL-33 and soluble form of IL-33 receptor (sST2) protein concentrations were measured in serum and sputum supernatants using ELISA. ST2 mRNA expression was measured in peripheral mononuclear cells and sputum cells by qPCR. Hemopoietic progenitor cells (HPC) expressing ST2 and intracellular IL-5 were enumerated in blood and induced sputum by means of flow cytometry. Results IL-33 levels in EBC were increased in COPD patients to a similar extent as in asthma and correlated with blood eosinophil count. Furthermore, serum and sputum IL-33 levels were higher in COPD subjects with sputum eosinophilia than in those with a sputum eosinophil count ≤3% (p < 0.001 for both). ST2 mRNA was overexpressed in sputum cells obtained from COPD patients with airway eosinophilic inflammation compared to those without sputum eosinophilia (p < 0.01). Similarly, ST2 + IL-5+ HPC numbers were increased in the sputum of COPD patients with airway eosinophilia (p < 0.001). Conclusions Our results indicate that IL-33 is involved in the development of eosinophilic airway inflammation in non-atopic COPD patients.
topic IL-33
Eosinophils
COPD
url http://link.springer.com/article/10.1186/s12931-018-0807-y
_version_ 1612699510884007936