The association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPD
Abstract Background Interleukin(IL)-33 is an epithelial alarmin important for eosinophil maturation, activation and survival. The aim of this study was to examine the association between IL-33, its receptor expression and airway eosinophilic inflammation in non-atopic COPD. Methods IL-33 concentrati...
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doaj-art-363279e872e44c93a78ebdf83c224d852018-08-15T23:43:47ZengBioMed CentralRespiratory Research1465-993X2018-06-0119111110.1186/s12931-018-0807-yThe association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPDDamian Tworek0Sebastian Majewski1Karolina Szewczyk2Justyna Kiszałkiewicz3Zofia Kurmanowska4Paweł Górski5Ewa Brzeziańska-Lasota6Piotr Kuna7Adam Antczak8Department of General and Oncological Pulmonology, Medical University of LodzDepartment of Pulmonology and Allergy, Medical University of LodzDepartment of Pulmonology and Allergy, Medical University of LodzDepartment of Biomedicine and Genetics, Medical University of LodzDepartment of Pulmonology and Allergy, Medical University of LodzDepartment of Pulmonology and Allergy, Medical University of LodzDepartment of Biomedicine and Genetics, Medical University of LodzDepartment of Internal Medicine, Asthma and Allergy, Medical University of LodzDepartment of General and Oncological Pulmonology, Medical University of LodzAbstract Background Interleukin(IL)-33 is an epithelial alarmin important for eosinophil maturation, activation and survival. The aim of this study was to examine the association between IL-33, its receptor expression and airway eosinophilic inflammation in non-atopic COPD. Methods IL-33 concentrations were measured in exhaled breath condensate (EBC) collected from healthy non-smokers, asthmatics and non-atopic COPD subjects using ELISA. Serum and sputum samples were collected from healthy non-smokers, healthy smokers and non-atopic COPD patients. Based on sputum eosinophil count, COPD subjects were divided into subgroups with airway eosinophilic inflammation (sputum eosinophils > 3%) or without (sputum eosinophils ≤3%). IL-33 and soluble form of IL-33 receptor (sST2) protein concentrations were measured in serum and sputum supernatants using ELISA. ST2 mRNA expression was measured in peripheral mononuclear cells and sputum cells by qPCR. Hemopoietic progenitor cells (HPC) expressing ST2 and intracellular IL-5 were enumerated in blood and induced sputum by means of flow cytometry. Results IL-33 levels in EBC were increased in COPD patients to a similar extent as in asthma and correlated with blood eosinophil count. Furthermore, serum and sputum IL-33 levels were higher in COPD subjects with sputum eosinophilia than in those with a sputum eosinophil count ≤3% (p < 0.001 for both). ST2 mRNA was overexpressed in sputum cells obtained from COPD patients with airway eosinophilic inflammation compared to those without sputum eosinophilia (p < 0.01). Similarly, ST2 + IL-5+ HPC numbers were increased in the sputum of COPD patients with airway eosinophilia (p < 0.001). Conclusions Our results indicate that IL-33 is involved in the development of eosinophilic airway inflammation in non-atopic COPD patients.http://link.springer.com/article/10.1186/s12931-018-0807-yIL-33EosinophilsCOPD |
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Damian Tworek Sebastian Majewski Karolina Szewczyk Justyna Kiszałkiewicz Zofia Kurmanowska Paweł Górski Ewa Brzeziańska-Lasota Piotr Kuna Adam Antczak |
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Damian Tworek Sebastian Majewski Karolina Szewczyk Justyna Kiszałkiewicz Zofia Kurmanowska Paweł Górski Ewa Brzeziańska-Lasota Piotr Kuna Adam Antczak The association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPD Respiratory Research IL-33 Eosinophils COPD |
author_facet |
Damian Tworek Sebastian Majewski Karolina Szewczyk Justyna Kiszałkiewicz Zofia Kurmanowska Paweł Górski Ewa Brzeziańska-Lasota Piotr Kuna Adam Antczak |
author_sort |
Damian Tworek |
title |
The association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPD |
title_short |
The association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPD |
title_full |
The association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPD |
title_fullStr |
The association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPD |
title_full_unstemmed |
The association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPD |
title_sort |
association between airway eosinophilic inflammation and il-33 in stable non-atopic copd |
publisher |
BioMed Central |
series |
Respiratory Research |
issn |
1465-993X |
publishDate |
2018-06-01 |
description |
Abstract Background Interleukin(IL)-33 is an epithelial alarmin important for eosinophil maturation, activation and survival. The aim of this study was to examine the association between IL-33, its receptor expression and airway eosinophilic inflammation in non-atopic COPD. Methods IL-33 concentrations were measured in exhaled breath condensate (EBC) collected from healthy non-smokers, asthmatics and non-atopic COPD subjects using ELISA. Serum and sputum samples were collected from healthy non-smokers, healthy smokers and non-atopic COPD patients. Based on sputum eosinophil count, COPD subjects were divided into subgroups with airway eosinophilic inflammation (sputum eosinophils > 3%) or without (sputum eosinophils ≤3%). IL-33 and soluble form of IL-33 receptor (sST2) protein concentrations were measured in serum and sputum supernatants using ELISA. ST2 mRNA expression was measured in peripheral mononuclear cells and sputum cells by qPCR. Hemopoietic progenitor cells (HPC) expressing ST2 and intracellular IL-5 were enumerated in blood and induced sputum by means of flow cytometry. Results IL-33 levels in EBC were increased in COPD patients to a similar extent as in asthma and correlated with blood eosinophil count. Furthermore, serum and sputum IL-33 levels were higher in COPD subjects with sputum eosinophilia than in those with a sputum eosinophil count ≤3% (p < 0.001 for both). ST2 mRNA was overexpressed in sputum cells obtained from COPD patients with airway eosinophilic inflammation compared to those without sputum eosinophilia (p < 0.01). Similarly, ST2 + IL-5+ HPC numbers were increased in the sputum of COPD patients with airway eosinophilia (p < 0.001). Conclusions Our results indicate that IL-33 is involved in the development of eosinophilic airway inflammation in non-atopic COPD patients. |
topic |
IL-33 Eosinophils COPD |
url |
http://link.springer.com/article/10.1186/s12931-018-0807-y |
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1612699510884007936 |