CHARACTERIZATION OF STAPHYLOCOCCAL CASSETTE CHROMOSOME MEC TYPES IN METHICILLIN-RESISTANT STAPHYLOCOCCUS INTERMEDIUS STRAINS ISOLATED FROM DOGS

SCCmec types in mecA-positive S. intermedius strains were characterized by using multiplex PCR method described previously by Oliveira and Lencastre [11]. A total of 100 preliminary identified S. intermedius isolates were tested to determine their species identify and mecA gene encoding methicillin...

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Main Authors:	Д. Хробак, М. Кiзерветтер-Свiда, М. Ржевуська, М. Бiнек
Format:	Article
Language:	English
Published:	Odessa I. I. Mechnikov National University 2009-09-01
Series:	Mìkrobìologìâ ì Bìotehnologìâ
Subjects:	Staphylococcus intermedius мультиплексна ПЛР mecA ген MRSI типи SCCmec
Online Access:	http://mbt.onu.edu.ua/article/view/103042

id	doaj-art-29958d31b5c24769aae1b14b5fe7ea68
recordtype	oai_dc
spelling	doaj-art-29958d31b5c24769aae1b14b5fe7ea682018-09-04T06:11:35ZengOdessa I. I. Mechnikov National University Mìkrobìologìâ ì Bìotehnologìâ2076-05582307-46632009-09-0103(7)495610.18524/2307-4663.2009.3(7).103042103042CHARACTERIZATION OF STAPHYLOCOCCAL CASSETTE CHROMOSOME MEC TYPES IN METHICILLIN-RESISTANT STAPHYLOCOCCUS INTERMEDIUS STRAINS ISOLATED FROM DOGSД. Хробак0М. Кiзерветтер-Свiда1М. Ржевуська2М. Бiнек3Варшавський університет природничих наукВаршавський університет природничих наукВаршавський університет природничих наукВаршавський університет природничих наукSCCmec types in mecA-positive S. intermedius strains were characterized by using multiplex PCR method described previously by Oliveira and Lencastre [11]. A total of 100 preliminary identified S. intermedius isolates were tested to determine their species identify and mecA gene encoding methicillin resistance. The duplex PCR assay was able to identity all the strains carring mecA gene (17%). The study showed that 4% strains identified as S. intermedius based upon their phenotypic properties do not yielded a 16S rRNA gene amplification product, indicating staphylococci other than S. intermedius. SCCmec type IIIC was identified in 15 strains (88%), type IB was found only in one strain. One strain was not typable by this technique.http://mbt.onu.edu.ua/article/view/103042Staphylococcus intermediusмультиплексна ПЛРmecA генMRSIтипи SCCmec
institution	Open Data Bank
collection	Open Access Journals
building	Directory of Open Access Journals
language	English
format	Article
author	Д. Хробак М. Кiзерветтер-Свiда М. Ржевуська М. Бiнек
spellingShingle	Д. Хробак М. Кiзерветтер-Свiда М. Ржевуська М. Бiнек CHARACTERIZATION OF STAPHYLOCOCCAL CASSETTE CHROMOSOME MEC TYPES IN METHICILLIN-RESISTANT STAPHYLOCOCCUS INTERMEDIUS STRAINS ISOLATED FROM DOGS Mìkrobìologìâ ì Bìotehnologìâ Staphylococcus intermedius мультиплексна ПЛР mecA ген MRSI типи SCCmec
author_facet	Д. Хробак М. Кiзерветтер-Свiда М. Ржевуська М. Бiнек
author_sort	Д. Хробак
title	CHARACTERIZATION OF STAPHYLOCOCCAL CASSETTE CHROMOSOME MEC TYPES IN METHICILLIN-RESISTANT STAPHYLOCOCCUS INTERMEDIUS STRAINS ISOLATED FROM DOGS
title_short	CHARACTERIZATION OF STAPHYLOCOCCAL CASSETTE CHROMOSOME MEC TYPES IN METHICILLIN-RESISTANT STAPHYLOCOCCUS INTERMEDIUS STRAINS ISOLATED FROM DOGS
title_full	CHARACTERIZATION OF STAPHYLOCOCCAL CASSETTE CHROMOSOME MEC TYPES IN METHICILLIN-RESISTANT STAPHYLOCOCCUS INTERMEDIUS STRAINS ISOLATED FROM DOGS
title_fullStr	CHARACTERIZATION OF STAPHYLOCOCCAL CASSETTE CHROMOSOME MEC TYPES IN METHICILLIN-RESISTANT STAPHYLOCOCCUS INTERMEDIUS STRAINS ISOLATED FROM DOGS
title_full_unstemmed	CHARACTERIZATION OF STAPHYLOCOCCAL CASSETTE CHROMOSOME MEC TYPES IN METHICILLIN-RESISTANT STAPHYLOCOCCUS INTERMEDIUS STRAINS ISOLATED FROM DOGS
title_sort	characterization of staphylococcal cassette chromosome mec types in methicillin-resistant staphylococcus intermedius strains isolated from dogs
publisher	Odessa I. I. Mechnikov National University
series	Mìkrobìologìâ ì Bìotehnologìâ
issn	2076-0558 2307-4663
publishDate	2009-09-01
description	SCCmec types in mecA-positive S. intermedius strains were characterized by using multiplex PCR method described previously by Oliveira and Lencastre [11]. A total of 100 preliminary identified S. intermedius isolates were tested to determine their species identify and mecA gene encoding methicillin resistance. The duplex PCR assay was able to identity all the strains carring mecA gene (17%). The study showed that 4% strains identified as S. intermedius based upon their phenotypic properties do not yielded a 16S rRNA gene amplification product, indicating staphylococci other than S. intermedius. SCCmec type IIIC was identified in 15 strains (88%), type IB was found only in one strain. One strain was not typable by this technique.
topic	Staphylococcus intermedius мультиплексна ПЛР mecA ген MRSI типи SCCmec
url	http://mbt.onu.edu.ua/article/view/103042
_version_	1612619313906188288

CHARACTERIZATION OF STAPHYLOCOCCAL CASSETTE CHROMOSOME MEC TYPES IN METHICILLIN-RESISTANT STAPHYLOCOCCUS INTERMEDIUS STRAINS ISOLATED FROM DOGS

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