A Sequel to Sanger: amplicon sequencing that scales

A Sequel to Sanger: amplicon sequencing that scales

Abstract Background Although high-throughput sequencers (HTS) have largely displaced their Sanger counterparts, the short read lengths and high error rates of most platforms constrain their utility for amplicon sequencing. The present study tests the capacity of single molecule, real-time (SMRT) seq...

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Bibliographic Details
Main Authors: Paul D. N. Hebert, Thomas W. A. Braukmann, Sean W. J. Prosser, Sujeevan Ratnasingham, Jeremy R. deWaard, Natalia V. Ivanova, Daniel H. Janzen, Winnie Hallwachs, Suresh Naik, Jayme E. Sones, Evgeny V. Zakharov
Format: Article
Language:English
Published: BioMed Central 2018-03-01
Series:BMC Genomics
Subjects:
SMRT sequencing
Mitochondrial DNA
Nuclear DNA
Phylogenetics
DNA barcoding
PCR
Online Access:http://link.springer.com/article/10.1186/s12864-018-4611-3

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