Survey for major viruses in commercial Vitis vinifera wine grapes in Ontario

Abstract Background In recent years, the Ontario grape and wine industry has experienced outbreaks of viral diseases across the province. Little is known about the prevalence of viruses and viral diseases in Ontario. Since 2015, we have conducted large-scale surveys for major viruses in commercial w...

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Main Authors:	Huogen Xiao, Mehdi Shabanian, Clayton Moore, Caihong Li, Baozhong Meng
Format:	Article
Language:	English
Published:	BioMed Central 2018-08-01
Series:	Virology Journal
Subjects:	Grapevine viral disease Grapevine leafroll Grapevine red blotch Grapevine rugose wood Grapevine fleck Vitis vinifera
Online Access:	http://link.springer.com/article/10.1186/s12985-018-1036-1

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recordtype	oai_dc
spelling	doaj-art-19952af432794d8cb2dd45c4ec42604b2018-08-16T01:35:19ZengBioMed CentralVirology Journal1743-422X2018-08-0115111110.1186/s12985-018-1036-1Survey for major viruses in commercial Vitis vinifera wine grapes in OntarioHuogen Xiao0Mehdi Shabanian1Clayton Moore2Caihong Li3Baozhong Meng4Department of Molecular and Cellular Biology, University of GuelphDepartment of Molecular and Cellular Biology, University of GuelphDepartment of Molecular and Cellular Biology, University of GuelphDepartment of Molecular and Cellular Biology, University of GuelphDepartment of Molecular and Cellular Biology, University of GuelphAbstract Background In recent years, the Ontario grape and wine industry has experienced outbreaks of viral diseases across the province. Little is known about the prevalence of viruses and viral diseases in Ontario. Since 2015, we have conducted large-scale surveys for major viruses in commercial wine grapes in order to obtain a comprehensive understanding of the prevalence and severity of viral diseases in Ontario. Methods A total of 657 composite leaf samples representing 3285 vines collected from 137 vine blocks of 33 vineyards from three appellations: Niagara Peninsula, Lake Erie North Shore and Prince Edward County. These samples covered six major red cultivars and five major white grape cultivars. Using a multiplex RT-PCR format, we tested these samples for 17 viruses including those involved in all major viral diseases of the grapevine, such as five grapevine leafroll-associated viruses (GLRaV-1, 2, 3, 4, 7), grapevine red blotch virus (GRBV), grapevine Pinot gris virus (GPGV), grapevine rupestris stem sitting-associated virus (GRSPaV), grapevine virus A (GVA), grapevine virus B (GVB), grapevine fleck virus (GFkV), arabis mosaic virus (ArMV), tomato ringspot virus (ToRSV), trapevine fanleaf virus (GFLV), among others. Results Fourteen of the 17 viruses were detected from these samples and the predominant viruses are GRSPaV, GLRaV-3, GFkV, GPGV and GRBaV with an incidence of 84.0, 47.9, 21.8, 21.6 and 18.3%, respectively. As expected, mixed infections with multiple viruses are common. 95.6% of the samples included in the survey were infected with at least one virus; 67% of the samples with 2–4 viruses and 4.7% of the samples with 5–6 viruses. The major grape cultivars all tested positive for these major viruses. The results also suggested that the use of infected planting material may have been one of the chief factors responsible for the recent outbreaks of viral diseases across the province. Conclusions This is the first such comprehensive survey for grapevine viruses in Ontario and one of the most extensive surveys ever conducted in Canada. The recent outbreaks of viral diseases in Ontario vineyards were likely caused by GLRaV-3, GRBV and GPGV. Findings from this survey provides a baseline for the grape and wine industry in developing strategies for managing grapevine viral diseases in Ontario vineyards.http://link.springer.com/article/10.1186/s12985-018-1036-1Grapevine viral diseaseGrapevine leafrollGrapevine red blotchGrapevine rugose woodGrapevine fleckVitis vinifera
institution	Open Data Bank
collection	Open Access Journals
building	Directory of Open Access Journals
language	English
format	Article
author	Huogen Xiao Mehdi Shabanian Clayton Moore Caihong Li Baozhong Meng
spellingShingle	Huogen Xiao Mehdi Shabanian Clayton Moore Caihong Li Baozhong Meng Survey for major viruses in commercial Vitis vinifera wine grapes in Ontario Virology Journal Grapevine viral disease Grapevine leafroll Grapevine red blotch Grapevine rugose wood Grapevine fleck Vitis vinifera
author_facet	Huogen Xiao Mehdi Shabanian Clayton Moore Caihong Li Baozhong Meng
author_sort	Huogen Xiao
title	Survey for major viruses in commercial Vitis vinifera wine grapes in Ontario
title_short	Survey for major viruses in commercial Vitis vinifera wine grapes in Ontario
title_full	Survey for major viruses in commercial Vitis vinifera wine grapes in Ontario
title_fullStr	Survey for major viruses in commercial Vitis vinifera wine grapes in Ontario
title_full_unstemmed	Survey for major viruses in commercial Vitis vinifera wine grapes in Ontario
title_sort	survey for major viruses in commercial vitis vinifera wine grapes in ontario
publisher	BioMed Central
series	Virology Journal
issn	1743-422X
publishDate	2018-08-01
description	Abstract Background In recent years, the Ontario grape and wine industry has experienced outbreaks of viral diseases across the province. Little is known about the prevalence of viruses and viral diseases in Ontario. Since 2015, we have conducted large-scale surveys for major viruses in commercial wine grapes in order to obtain a comprehensive understanding of the prevalence and severity of viral diseases in Ontario. Methods A total of 657 composite leaf samples representing 3285 vines collected from 137 vine blocks of 33 vineyards from three appellations: Niagara Peninsula, Lake Erie North Shore and Prince Edward County. These samples covered six major red cultivars and five major white grape cultivars. Using a multiplex RT-PCR format, we tested these samples for 17 viruses including those involved in all major viral diseases of the grapevine, such as five grapevine leafroll-associated viruses (GLRaV-1, 2, 3, 4, 7), grapevine red blotch virus (GRBV), grapevine Pinot gris virus (GPGV), grapevine rupestris stem sitting-associated virus (GRSPaV), grapevine virus A (GVA), grapevine virus B (GVB), grapevine fleck virus (GFkV), arabis mosaic virus (ArMV), tomato ringspot virus (ToRSV), trapevine fanleaf virus (GFLV), among others. Results Fourteen of the 17 viruses were detected from these samples and the predominant viruses are GRSPaV, GLRaV-3, GFkV, GPGV and GRBaV with an incidence of 84.0, 47.9, 21.8, 21.6 and 18.3%, respectively. As expected, mixed infections with multiple viruses are common. 95.6% of the samples included in the survey were infected with at least one virus; 67% of the samples with 2–4 viruses and 4.7% of the samples with 5–6 viruses. The major grape cultivars all tested positive for these major viruses. The results also suggested that the use of infected planting material may have been one of the chief factors responsible for the recent outbreaks of viral diseases across the province. Conclusions This is the first such comprehensive survey for grapevine viruses in Ontario and one of the most extensive surveys ever conducted in Canada. The recent outbreaks of viral diseases in Ontario vineyards were likely caused by GLRaV-3, GRBV and GPGV. Findings from this survey provides a baseline for the grape and wine industry in developing strategies for managing grapevine viral diseases in Ontario vineyards.
topic	Grapevine viral disease Grapevine leafroll Grapevine red blotch Grapevine rugose wood Grapevine fleck Vitis vinifera
url	http://link.springer.com/article/10.1186/s12985-018-1036-1
_version_	1612694386514067456

Survey for major viruses in commercial Vitis vinifera wine grapes in Ontario

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