Antioxidant and Free Radical Scavenging Activity of Trigonella foenum-graecum L, Murraya koenigii , Coriandrum sativum and Centella asiatica

Antioxidant and Free Radical Scavenging Activity of Trigonella foenum-graecum L, Murraya koenigii , Coriandrum sativum and Centella asiatica

Antioxidants are naturally occurring substances that combat oxidative damage in biological entities. An antioxidant achieves this by slowing or preventing the oxidation process that can damage cells in the body. It does this by getting oxidized itself in place of the cells. The aim of the present s...

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Main Authors: Sanghamitra Dutta, Debanita Roy, Archisman De, Camellia Dutta, Swagata Bhattacharya
Format: Article
Language:English
Published: Turkish Science and Tecnology (TST) 2016-04-01
Series:Turkish Journal of Agriculture: Food Science and Technology
Subjects:
culinary herbs
leaf extract, antioxidants
free radical scavenger
Online Access:http://www.agrifoodscience.com/index.php/TURJAF/article/view/520
id doaj-art-12ed7487c953498593936735df9edbc9
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spelling doaj-art-12ed7487c953498593936735df9edbc92018-09-09T11:53:38ZengTurkish Science and Tecnology (TST)Turkish Journal of Agriculture: Food Science and Technology2148-127X2016-04-014425526110.24925/turjaf.v4i4.255-261.520298Antioxidant and Free Radical Scavenging Activity of Trigonella foenum-graecum L, Murraya koenigii , Coriandrum sativum and Centella asiaticaSanghamitra Dutta0Debanita Roy1Archisman De2Camellia Dutta3Swagata Bhattacharya4Bengal Institute of TechnologyBengal Institute of TechnologyBengal Institute of TechnologyBengal Institute of TechnologyBengal Institute of TechnologyAntioxidants are naturally occurring substances that combat oxidative damage in biological entities. An antioxidant achieves this by slowing or preventing the oxidation process that can damage cells in the body. It does this by getting oxidized itself in place of the cells. The aim of the present study was to evaluate the in vitro antioxidant and free radical scavenging activities of aqueous and 95% methanol leaf extracts of four herbs viz. Trigonella foenum-graecum L, Murraya koenigii, Coriandrum sativum and Centella asiatica which have frequent use in Indian cuisine. Both aqueous and 95% methanol leaf extracts have shown significant amount reducing power. Both aqueous and 95% methanol leaf extracts of Coriandrum sativum had significant DPPH radical scavenging activity with IC50 value of 0.21± 0.3 mg/L and 0.176 ± 0.008 mg/L respectively. The aqueous leaf extract of Trigonella foenum-graecum L showed low scavenging activity. Among all the leaf extracts, the aqueous leaf extract of Centella asiatica has exhibited significantly high NO radical scavenging activity (80%) with IC50 value of 0.11 ± 0.17 mg/L. The aqueous leaf extracts of the samples have showed significantly high superoxide radical scavenging activity. The activity was maximum for the aqueous leaf extract of Centella asiatica, IC50 value is 4.36 ± 0.41 mg/L. anti lipid peroxide activities were very high ( 90 %) for aqueous leaf extracts of Coriandrum sativum (IC50 = 0.064 ± 0.85 mg/L) and Centella asiatica (IC50 = 0.066 ± 0.9mg/L) at a concentration of 0.16 mg/L. The aqueous leaf extracts of the samples were found to contain large amounts of flavonoids and phenolic compounds and exhibited high antioxidant and free radical scavenging activities. These in vitro assays indicate that these plant extracts are significant source of natural antioxidants which might be helpful in preventing the progress of various oxidative stresses.http://www.agrifoodscience.com/index.php/TURJAF/article/view/520culinary herbsleaf extract, antioxidantsfree radical scavenger
institution Open Data Bank
collection Open Access Journals
building Directory of Open Access Journals
language English
format Article
author Sanghamitra Dutta
Debanita Roy
Archisman De
Camellia Dutta
Swagata Bhattacharya
spellingShingle Sanghamitra Dutta
Debanita Roy
Archisman De
Camellia Dutta
Swagata Bhattacharya
Antioxidant and Free Radical Scavenging Activity of Trigonella foenum-graecum L, Murraya koenigii , Coriandrum sativum and Centella asiatica
Turkish Journal of Agriculture: Food Science and Technology
culinary herbs
leaf extract, antioxidants
free radical scavenger
author_facet Sanghamitra Dutta
Debanita Roy
Archisman De
Camellia Dutta
Swagata Bhattacharya
author_sort Sanghamitra Dutta
title Antioxidant and Free Radical Scavenging Activity of Trigonella foenum-graecum L, Murraya koenigii , Coriandrum sativum and Centella asiatica
title_short Antioxidant and Free Radical Scavenging Activity of Trigonella foenum-graecum L, Murraya koenigii , Coriandrum sativum and Centella asiatica
title_full Antioxidant and Free Radical Scavenging Activity of Trigonella foenum-graecum L, Murraya koenigii , Coriandrum sativum and Centella asiatica
title_fullStr Antioxidant and Free Radical Scavenging Activity of Trigonella foenum-graecum L, Murraya koenigii , Coriandrum sativum and Centella asiatica
title_full_unstemmed Antioxidant and Free Radical Scavenging Activity of Trigonella foenum-graecum L, Murraya koenigii , Coriandrum sativum and Centella asiatica
title_sort antioxidant and free radical scavenging activity of trigonella foenum-graecum l, murraya koenigii , coriandrum sativum and centella asiatica
publisher Turkish Science and Tecnology (TST)
series Turkish Journal of Agriculture: Food Science and Technology
issn 2148-127X
publishDate 2016-04-01
description Antioxidants are naturally occurring substances that combat oxidative damage in biological entities. An antioxidant achieves this by slowing or preventing the oxidation process that can damage cells in the body. It does this by getting oxidized itself in place of the cells. The aim of the present study was to evaluate the in vitro antioxidant and free radical scavenging activities of aqueous and 95% methanol leaf extracts of four herbs viz. Trigonella foenum-graecum L, Murraya koenigii, Coriandrum sativum and Centella asiatica which have frequent use in Indian cuisine. Both aqueous and 95% methanol leaf extracts have shown significant amount reducing power. Both aqueous and 95% methanol leaf extracts of Coriandrum sativum had significant DPPH radical scavenging activity with IC50 value of 0.21± 0.3 mg/L and 0.176 ± 0.008 mg/L respectively. The aqueous leaf extract of Trigonella foenum-graecum L showed low scavenging activity. Among all the leaf extracts, the aqueous leaf extract of Centella asiatica has exhibited significantly high NO radical scavenging activity (80%) with IC50 value of 0.11 ± 0.17 mg/L. The aqueous leaf extracts of the samples have showed significantly high superoxide radical scavenging activity. The activity was maximum for the aqueous leaf extract of Centella asiatica, IC50 value is 4.36 ± 0.41 mg/L. anti lipid peroxide activities were very high ( 90 %) for aqueous leaf extracts of Coriandrum sativum (IC50 = 0.064 ± 0.85 mg/L) and Centella asiatica (IC50 = 0.066 ± 0.9mg/L) at a concentration of 0.16 mg/L. The aqueous leaf extracts of the samples were found to contain large amounts of flavonoids and phenolic compounds and exhibited high antioxidant and free radical scavenging activities. These in vitro assays indicate that these plant extracts are significant source of natural antioxidants which might be helpful in preventing the progress of various oxidative stresses.
topic culinary herbs
leaf extract, antioxidants
free radical scavenger
url http://www.agrifoodscience.com/index.php/TURJAF/article/view/520
_version_ 1612608978175393792

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