Identification and characterisation of seed storage protein transcripts from Lupinus angustifolius

Background: In legumes, seed storage proteins are important for the developing seedling and are an important source of protein for humans and animals. Lupinus angustifolius (L.), also known as narrow-leaf lupin (NLL) is a grain legume crop that is gaining recognition as a potential human health food...

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Main Authors:	Foley, R., Gao, L., Spriggs, A., Soo, L., Goggin, D., Smith, P., Atkins, C., Singh, Karambir
Format:	Journal Article
Published:	BioMed Central Ltd 2011
Online Access:	http://hdl.handle.net/20.500.11937/8103

id	curtin-20.500.11937-8103
recordtype	eprints
spelling	curtin-20.500.11937-81032017-09-13T14:35:06Z Identification and characterisation of seed storage protein transcripts from Lupinus angustifolius Foley, R. Gao, L. Spriggs, A. Soo, L. Goggin, D. Smith, P. Atkins, C. Singh, Karambir Background: In legumes, seed storage proteins are important for the developing seedling and are an important source of protein for humans and animals. Lupinus angustifolius (L.), also known as narrow-leaf lupin (NLL) is a grain legume crop that is gaining recognition as a potential human health food as the grain is high in protein and dietary fibre, gluten-free and low in fat and starch.Results: Genes encoding the seed storage proteins of NLL were characterised by sequencing cDNA clones derived from developing seeds. Four families of seed storage proteins were identified and comprised three unique a, seven ß, two ? and four d conglutins. This study added eleven new expressed storage protein genes for the species. A comparison of the deduced amino acid sequences of NLL conglutins with those available for the storage proteins of Lupinus albus (L.), Pisum sativum (L.), Medicago truncatula (L.), Arachis hypogaea (L.) and Glycine max (L.) permitted the analysis of a phylogenetic relationships between proteins and demonstrated, in general, that the strongest conservation occurred within species. In the case of 7S globulin (ß conglutins) and 2S sulphur-rich albumin (d conglutins), the analysis suggests that gene duplication occurred after legume speciation. This contrasted with 11S globulin (a conglutin) and basic 7S (? conglutin) sequences where some of these sequences appear to have diverged prior to speciation. The most abundant NLL conglutin family was ß (56%), followed by a (24%), d (15%) and ? (6%) and the transcript levels of these genes increased 103 to 106 fold during seed development. We used the 16 NLL conglutin sequences identified here to determine that for individuals specifically allergic to lupin, all seven members of the ß conglutin family were potential allergens.Conclusion: This study has characterised 16 seed storage protein genes in NLL including 11 newly-identified members. It has helped lay the foundation for efforts to use molecular breeding approaches to improve lupins, for example by reducing allergens or increasing the expression of specific seed storage protein(s) with desirable nutritional properties. 2011 Journal Article http://hdl.handle.net/20.500.11937/8103 10.1186/1471-2229-11-59 BioMed Central Ltd unknown
repository_type	Digital Repository
institution_category	Local University
institution	Curtin University Malaysia
building	Curtin Institutional Repository
collection	Online Access
description	Background: In legumes, seed storage proteins are important for the developing seedling and are an important source of protein for humans and animals. Lupinus angustifolius (L.), also known as narrow-leaf lupin (NLL) is a grain legume crop that is gaining recognition as a potential human health food as the grain is high in protein and dietary fibre, gluten-free and low in fat and starch.Results: Genes encoding the seed storage proteins of NLL were characterised by sequencing cDNA clones derived from developing seeds. Four families of seed storage proteins were identified and comprised three unique a, seven ß, two ? and four d conglutins. This study added eleven new expressed storage protein genes for the species. A comparison of the deduced amino acid sequences of NLL conglutins with those available for the storage proteins of Lupinus albus (L.), Pisum sativum (L.), Medicago truncatula (L.), Arachis hypogaea (L.) and Glycine max (L.) permitted the analysis of a phylogenetic relationships between proteins and demonstrated, in general, that the strongest conservation occurred within species. In the case of 7S globulin (ß conglutins) and 2S sulphur-rich albumin (d conglutins), the analysis suggests that gene duplication occurred after legume speciation. This contrasted with 11S globulin (a conglutin) and basic 7S (? conglutin) sequences where some of these sequences appear to have diverged prior to speciation. The most abundant NLL conglutin family was ß (56%), followed by a (24%), d (15%) and ? (6%) and the transcript levels of these genes increased 103 to 106 fold during seed development. We used the 16 NLL conglutin sequences identified here to determine that for individuals specifically allergic to lupin, all seven members of the ß conglutin family were potential allergens.Conclusion: This study has characterised 16 seed storage protein genes in NLL including 11 newly-identified members. It has helped lay the foundation for efforts to use molecular breeding approaches to improve lupins, for example by reducing allergens or increasing the expression of specific seed storage protein(s) with desirable nutritional properties.
format	Journal Article
author	Foley, R. Gao, L. Spriggs, A. Soo, L. Goggin, D. Smith, P. Atkins, C. Singh, Karambir
spellingShingle	Foley, R. Gao, L. Spriggs, A. Soo, L. Goggin, D. Smith, P. Atkins, C. Singh, Karambir Identification and characterisation of seed storage protein transcripts from Lupinus angustifolius
author_facet	Foley, R. Gao, L. Spriggs, A. Soo, L. Goggin, D. Smith, P. Atkins, C. Singh, Karambir
author_sort	Foley, R.
title	Identification and characterisation of seed storage protein transcripts from Lupinus angustifolius
title_short	Identification and characterisation of seed storage protein transcripts from Lupinus angustifolius
title_full	Identification and characterisation of seed storage protein transcripts from Lupinus angustifolius
title_fullStr	Identification and characterisation of seed storage protein transcripts from Lupinus angustifolius
title_full_unstemmed	Identification and characterisation of seed storage protein transcripts from Lupinus angustifolius
title_sort	identification and characterisation of seed storage protein transcripts from lupinus angustifolius
publisher	BioMed Central Ltd
publishDate	2011
url	http://hdl.handle.net/20.500.11937/8103
first_indexed	2018-09-06T18:23:07Z
last_indexed	2018-09-06T18:23:07Z
_version_	1610883465385869312

Identification and characterisation of seed storage protein transcripts from Lupinus angustifolius

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