Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture

Sucrose has several advantages over glucose as a feedstock for bioprocesses, both environmentally and economically. However, most industrial Escherichia coli strains are unable to utilize sucrose. E. coli W can grow on sucrose but stops growing when sucrose concentrations become low. This is undesir...

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Main Authors:	Arifin, Yalun, Sabri, S., Sugiarto, H., Krömer, J., Vickers, C., Nielsen, L.
Format:	Journal Article
Published:	Elsevier Science 2010
Online Access:	http://hdl.handle.net/20.500.11937/6726

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recordtype	eprints
spelling	curtin-20.500.11937-67262017-09-13T14:35:42Z Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture Arifin, Yalun Sabri, S. Sugiarto, H. Krömer, J. Vickers, C. Nielsen, L. Sucrose has several advantages over glucose as a feedstock for bioprocesses, both environmentally and economically. However, most industrial Escherichia coli strains are unable to utilize sucrose. E. coli W can grow on sucrose but stops growing when sucrose concentrations become low. This is undesirable in fed-batch conditions where sugar levels are low between feeding pulses. Sucrose uptake rates were improved by removal of the cscR gene, which encodes a protein that represses expression of the sucrose utilization genes at low sucrose concentrations. Poly-3-hydroxybutyrate (PHB) was used as a model compound in order to assess the effect of improved sugar utilization on bio-production. In the cscR knockout strain, production from sucrose was improved by 50%; this strain also produced 30% more PHB than the wild-type using glucose. This result demonstrates the feasibility of utilizing sucrose as an industrial feedstock for E. coli-based bioprocesses in high cell density culture. © 2011. 2010 Journal Article http://hdl.handle.net/20.500.11937/6726 10.1016/j.jbiotec.2011.07.003 Elsevier Science restricted
repository_type	Digital Repository
institution_category	Local University
institution	Curtin University Malaysia
building	Curtin Institutional Repository
collection	Online Access
description	Sucrose has several advantages over glucose as a feedstock for bioprocesses, both environmentally and economically. However, most industrial Escherichia coli strains are unable to utilize sucrose. E. coli W can grow on sucrose but stops growing when sucrose concentrations become low. This is undesirable in fed-batch conditions where sugar levels are low between feeding pulses. Sucrose uptake rates were improved by removal of the cscR gene, which encodes a protein that represses expression of the sucrose utilization genes at low sucrose concentrations. Poly-3-hydroxybutyrate (PHB) was used as a model compound in order to assess the effect of improved sugar utilization on bio-production. In the cscR knockout strain, production from sucrose was improved by 50%; this strain also produced 30% more PHB than the wild-type using glucose. This result demonstrates the feasibility of utilizing sucrose as an industrial feedstock for E. coli-based bioprocesses in high cell density culture. © 2011.
format	Journal Article
author	Arifin, Yalun Sabri, S. Sugiarto, H. Krömer, J. Vickers, C. Nielsen, L.
spellingShingle	Arifin, Yalun Sabri, S. Sugiarto, H. Krömer, J. Vickers, C. Nielsen, L. Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture
author_facet	Arifin, Yalun Sabri, S. Sugiarto, H. Krömer, J. Vickers, C. Nielsen, L.
author_sort	Arifin, Yalun
title	Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture
title_short	Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture
title_full	Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture
title_fullStr	Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture
title_full_unstemmed	Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture
title_sort	deletion of cscr in escherichia coli w improves growth and poly-3-hydroxybutyrate (phb) production from sucrose in fed batch culture
publisher	Elsevier Science
publishDate	2010
url	http://hdl.handle.net/20.500.11937/6726
first_indexed	2018-09-06T18:11:18Z
last_indexed	2018-09-06T18:11:18Z
_version_	1610882722050342912

Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture

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