The B-3 Ethylene Response Factor MtERF1-1 Mediates Resistance to a Subset of Root Pathogens in Medicago truncatula without Adversely Affecting Symbiosis with Rhizobia

The fungal necrotrophic pathogen Rhizoctonia solani is a significant constraint to a range of crops as diverse as cereals, canola,and legumes. Despite wide-ranging germplasm screens in many of these crops, no strong genetic resistance has beenidentified, suggesting that alternative strategies to imp...

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Main Authors:	Anderson, J., Lichtenzveig, Judith, Gleason, C., Oliver, Richard, Singh, K.
Format:	Journal Article
Published:	American Society of Plant Biologists 2010
Online Access:	http://hdl.handle.net/20.500.11937/44878

id	curtin-20.500.11937-44878
recordtype	eprints
spelling	curtin-20.500.11937-448782017-02-28T01:37:51Z The B-3 Ethylene Response Factor MtERF1-1 Mediates Resistance to a Subset of Root Pathogens in Medicago truncatula without Adversely Affecting Symbiosis with Rhizobia Anderson, J. Lichtenzveig, Judith Gleason, C. Oliver, Richard Singh, K. The fungal necrotrophic pathogen Rhizoctonia solani is a significant constraint to a range of crops as diverse as cereals, canola,and legumes. Despite wide-ranging germplasm screens in many of these crops, no strong genetic resistance has beenidentified, suggesting that alternative strategies to improve resistance are required. In this study, we characterize moderateresistance to R. solani anastomosis group 8 identified in Medicago truncatula. The activity of the ethylene- and jasmonateresponsiveGCC box promoter element was associated with moderate resistance, as was the induction of the B-3 subgroup ofethylene response transcription factors (ERFs). Genes of the B-1 subgroup showed no significant response to R. solani infection.Overexpression of a B-3 ERF, MtERF1-1, in Medicago roots increased resistance to R. solani as well as an oomycete rootpathogen, Phytophthora medicaginis, but not root knot nematode. These results indicate that targeting specific regulators ofethylene defense may enhance resistance to an important subset of root pathogens.We also demonstrate that overexpression ofMtERF1-1 enhances disease resistance without apparent impact on nodulation in the A17 background, while overexpression insickle reduced the hypernodulation phenotype. This suggests that under normal regulation of nodulation, enhanced resistanceto root diseases can be uncoupled from symbiotic plant-microbe interactions in the same tissue and that ethylene/ERFregulation of nodule number is distinct from the defenses regulated by B-3 ERFs. Furthermore, unlike the stunted phenotypepreviously described for Arabidopsis (Arabidopsis thaliana) ubiquitously overexpressing B-3 ERFs, overexpression of MtERF1-1 inM. truncatula roots did not show adverse effects on plant development. 2010 Journal Article http://hdl.handle.net/20.500.11937/44878 American Society of Plant Biologists restricted
repository_type	Digital Repository
institution_category	Local University
institution	Curtin University Malaysia
building	Curtin Institutional Repository
collection	Online Access
description	The fungal necrotrophic pathogen Rhizoctonia solani is a significant constraint to a range of crops as diverse as cereals, canola,and legumes. Despite wide-ranging germplasm screens in many of these crops, no strong genetic resistance has beenidentified, suggesting that alternative strategies to improve resistance are required. In this study, we characterize moderateresistance to R. solani anastomosis group 8 identified in Medicago truncatula. The activity of the ethylene- and jasmonateresponsiveGCC box promoter element was associated with moderate resistance, as was the induction of the B-3 subgroup ofethylene response transcription factors (ERFs). Genes of the B-1 subgroup showed no significant response to R. solani infection.Overexpression of a B-3 ERF, MtERF1-1, in Medicago roots increased resistance to R. solani as well as an oomycete rootpathogen, Phytophthora medicaginis, but not root knot nematode. These results indicate that targeting specific regulators ofethylene defense may enhance resistance to an important subset of root pathogens.We also demonstrate that overexpression ofMtERF1-1 enhances disease resistance without apparent impact on nodulation in the A17 background, while overexpression insickle reduced the hypernodulation phenotype. This suggests that under normal regulation of nodulation, enhanced resistanceto root diseases can be uncoupled from symbiotic plant-microbe interactions in the same tissue and that ethylene/ERFregulation of nodule number is distinct from the defenses regulated by B-3 ERFs. Furthermore, unlike the stunted phenotypepreviously described for Arabidopsis (Arabidopsis thaliana) ubiquitously overexpressing B-3 ERFs, overexpression of MtERF1-1 inM. truncatula roots did not show adverse effects on plant development.
format	Journal Article
author	Anderson, J. Lichtenzveig, Judith Gleason, C. Oliver, Richard Singh, K.
spellingShingle	Anderson, J. Lichtenzveig, Judith Gleason, C. Oliver, Richard Singh, K. The B-3 Ethylene Response Factor MtERF1-1 Mediates Resistance to a Subset of Root Pathogens in Medicago truncatula without Adversely Affecting Symbiosis with Rhizobia
author_facet	Anderson, J. Lichtenzveig, Judith Gleason, C. Oliver, Richard Singh, K.
author_sort	Anderson, J.
title	The B-3 Ethylene Response Factor MtERF1-1 Mediates Resistance to a Subset of Root Pathogens in Medicago truncatula without Adversely Affecting Symbiosis with Rhizobia
title_short	The B-3 Ethylene Response Factor MtERF1-1 Mediates Resistance to a Subset of Root Pathogens in Medicago truncatula without Adversely Affecting Symbiosis with Rhizobia
title_full	The B-3 Ethylene Response Factor MtERF1-1 Mediates Resistance to a Subset of Root Pathogens in Medicago truncatula without Adversely Affecting Symbiosis with Rhizobia
title_fullStr	The B-3 Ethylene Response Factor MtERF1-1 Mediates Resistance to a Subset of Root Pathogens in Medicago truncatula without Adversely Affecting Symbiosis with Rhizobia
title_full_unstemmed	The B-3 Ethylene Response Factor MtERF1-1 Mediates Resistance to a Subset of Root Pathogens in Medicago truncatula without Adversely Affecting Symbiosis with Rhizobia
title_sort	b-3 ethylene response factor mterf1-1 mediates resistance to a subset of root pathogens in medicago truncatula without adversely affecting symbiosis with rhizobia
publisher	American Society of Plant Biologists
publishDate	2010
url	http://hdl.handle.net/20.500.11937/44878
first_indexed	2018-09-06T23:41:29Z
last_indexed	2018-09-06T23:41:29Z
_version_	1610903495093780480

The B-3 Ethylene Response Factor MtERF1-1 Mediates Resistance to a Subset of Root Pathogens in Medicago truncatula without Adversely Affecting Symbiosis with Rhizobia

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