Identification of distinct quantitative trait loci associated with defense against the closely related aphids Acyrthosiphon pisum and A. kondoi in Medicago truncatula

Aphids are a major family of plant insect pests. Medicago truncatula and Acyrthosiphon pisum (pea aphid, PA) are model species with a suite of resources available to help dissect the mechanism underlying plant–aphid interactions. A previous study focused on monogenic and relatively strong resistance...

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Main Authors:	Guo, Su-Min, Kamphuis, Lars, Gao, Ling-Ling, Klingler, John p, Lichtenzveig, Judith, Edwards, Owain, Singh, Karam
Format:	Journal Article
Published:	Oxford University Press 2012
Subjects:	hypersensitive response sap-sucking insect antixenosis herbivory Antibiosis necrosis EPG phloem
Online Access:	http://hdl.handle.net/20.500.11937/43023

id	curtin-20.500.11937-43023
recordtype	eprints
spelling	curtin-20.500.11937-430232017-09-13T16:07:08Z Identification of distinct quantitative trait loci associated with defense against the closely related aphids Acyrthosiphon pisum and A. kondoi in Medicago truncatula Guo, Su-Min Kamphuis, Lars Gao, Ling-Ling Klingler, John p Lichtenzveig, Judith Edwards, Owain Singh, Karam hypersensitive response sap-sucking insect antixenosis herbivory Antibiosis necrosis EPG phloem Aphids are a major family of plant insect pests. Medicago truncatula and Acyrthosiphon pisum (pea aphid, PA) are model species with a suite of resources available to help dissect the mechanism underlying plant–aphid interactions. A previous study focused on monogenic and relatively strong resistance in M. truncatula to PA and other aphid species. In this study a moderate resistance to PA was characterized in detail in the M. truncatula line A17 and compared with the highly susceptible line A20 and the more resistant line Jester. The results show that PA resistance in A17 involves both antibiosis and tolerance, and that resistance is phloem based. Quantitative trait locus (QTL) analysis using a recombinant inbred line (RIL) population (n=114) from a cross between A17 and A20 revealed that one locus, which co-segregated with AIN (Acyrthosiphon-induced necrosis) on chromosome 3, is responsible for the reduction of aphid biomass (indicator of antibiosis) for both PA and bluegreen aphid (BGA, A. kondoi), albeit to a lesser degree for PA than BGA. Interestingly, two independent loci on chromosomes 5 and 3 were identified for the plant biomass reduction (indicator of plant tolerance) by PA and BGA, respectively, demonstrating that the plant’s tolerance response to these two closely related aphid species is distinct. Together with previously identified major resistant (R) genes, the QTLs identified in this study are powerful tools to understand fully the spectrum of plant defence against sap-sucking insects and provide opportunities for breeders to generate effective and sustainable strategies for aphid control. 2012 Journal Article http://hdl.handle.net/20.500.11937/43023 10.1093/jxb/ers084 Oxford University Press fulltext
repository_type	Digital Repository
institution_category	Local University
institution	Curtin University Malaysia
building	Curtin Institutional Repository
collection	Online Access
topic	hypersensitive response sap-sucking insect antixenosis herbivory Antibiosis necrosis EPG phloem
spellingShingle	hypersensitive response sap-sucking insect antixenosis herbivory Antibiosis necrosis EPG phloem Guo, Su-Min Kamphuis, Lars Gao, Ling-Ling Klingler, John p Lichtenzveig, Judith Edwards, Owain Singh, Karam Identification of distinct quantitative trait loci associated with defense against the closely related aphids Acyrthosiphon pisum and A. kondoi in Medicago truncatula
description	Aphids are a major family of plant insect pests. Medicago truncatula and Acyrthosiphon pisum (pea aphid, PA) are model species with a suite of resources available to help dissect the mechanism underlying plant–aphid interactions. A previous study focused on monogenic and relatively strong resistance in M. truncatula to PA and other aphid species. In this study a moderate resistance to PA was characterized in detail in the M. truncatula line A17 and compared with the highly susceptible line A20 and the more resistant line Jester. The results show that PA resistance in A17 involves both antibiosis and tolerance, and that resistance is phloem based. Quantitative trait locus (QTL) analysis using a recombinant inbred line (RIL) population (n=114) from a cross between A17 and A20 revealed that one locus, which co-segregated with AIN (Acyrthosiphon-induced necrosis) on chromosome 3, is responsible for the reduction of aphid biomass (indicator of antibiosis) for both PA and bluegreen aphid (BGA, A. kondoi), albeit to a lesser degree for PA than BGA. Interestingly, two independent loci on chromosomes 5 and 3 were identified for the plant biomass reduction (indicator of plant tolerance) by PA and BGA, respectively, demonstrating that the plant’s tolerance response to these two closely related aphid species is distinct. Together with previously identified major resistant (R) genes, the QTLs identified in this study are powerful tools to understand fully the spectrum of plant defence against sap-sucking insects and provide opportunities for breeders to generate effective and sustainable strategies for aphid control.
format	Journal Article
author	Guo, Su-Min Kamphuis, Lars Gao, Ling-Ling Klingler, John p Lichtenzveig, Judith Edwards, Owain Singh, Karam
author_facet	Guo, Su-Min Kamphuis, Lars Gao, Ling-Ling Klingler, John p Lichtenzveig, Judith Edwards, Owain Singh, Karam
author_sort	Guo, Su-Min
title	Identification of distinct quantitative trait loci associated with defense against the closely related aphids Acyrthosiphon pisum and A. kondoi in Medicago truncatula
title_short	Identification of distinct quantitative trait loci associated with defense against the closely related aphids Acyrthosiphon pisum and A. kondoi in Medicago truncatula
title_full	Identification of distinct quantitative trait loci associated with defense against the closely related aphids Acyrthosiphon pisum and A. kondoi in Medicago truncatula
title_fullStr	Identification of distinct quantitative trait loci associated with defense against the closely related aphids Acyrthosiphon pisum and A. kondoi in Medicago truncatula
title_full_unstemmed	Identification of distinct quantitative trait loci associated with defense against the closely related aphids Acyrthosiphon pisum and A. kondoi in Medicago truncatula
title_sort	identification of distinct quantitative trait loci associated with defense against the closely related aphids acyrthosiphon pisum and a. kondoi in medicago truncatula
publisher	Oxford University Press
publishDate	2012
url	http://hdl.handle.net/20.500.11937/43023
first_indexed	2018-09-06T23:25:31Z
last_indexed	2018-09-06T23:25:31Z
_version_	1610902490463600640

Identification of distinct quantitative trait loci associated with defense against the closely related aphids Acyrthosiphon pisum and A. kondoi in Medicago truncatula

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