Enzymatic treatment of methyl orange dye in synthetic wastewater by plant-based peroxidase enzymes

The search for effective and sustainable methods to degrade and remove recalcitrant dyes from textile effluents is a major research endeavor, owing to the escalating environmental and health concerns arising from the discharge of coloured effluents into water bodies. Plant-based peroxidases represen...

Full description

Bibliographic Details
Main Authors:	Chiong, T., Lau, John, Lek, Z., Koh, B., Danquah, M.
Format:	Journal Article
Published:	Elsevuer BV 2016
Online Access:	http://hdl.handle.net/20.500.11937/35158

id	curtin-20.500.11937-35158
recordtype	eprints
spelling	curtin-20.500.11937-351582017-09-13T15:32:25Z Enzymatic treatment of methyl orange dye in synthetic wastewater by plant-based peroxidase enzymes Chiong, T. Lau, John Lek, Z. Koh, B. Danquah, M. The search for effective and sustainable methods to degrade and remove recalcitrant dyes from textile effluents is a major research endeavor, owing to the escalating environmental and health concerns arising from the discharge of coloured effluents into water bodies. Plant-based peroxidases represent a reliable bio-resource for sustainable treatment of coloured effluents with the capacity to offer a continuous process for high throughput operation. The present study investigates the potential use of soybean peroxidase and Luffa acutangula (luffa) peroxidase, extracted from bio-wastes of soybean hulls and luffa skin peels respectively, for enzymatic degradation of azo dye methyl orange from liquid effluents. The enzymatic dye removal process was studied based on the consistent enzymatic activities of crude soybean peroxidase and luffa peroxidase extracts, which were 0.373 U mL-1 and 0.355 U mL-1 respectively. The effects of several process parameters including reaction time, pH, temperature, enzyme dosage, initial dye concentration and hydrogen peroxide concentration were investigated to optimise the performance of the enzymatic treatment process. Soybean peroxidase demonstrated a maximum dye decolourisation efficiency of 81.4% under the conditions of 1 h incubation at 30 °C using 2 mM of hydrogen peroxide, 0.5 mL crude soybean peroxidase and 30 mg L-1 methyl orange at pH 5.0. Also, luffa peroxidase yielded a maximum decolourisation efficiency of 75.3% under the conditions of 40 min at 40 °C using 2 mM hydrogen peroxide, 1.5 mL crude luffa peroxidase and 10 mg L-1 methyl orange at pH 3.0. 2016 Journal Article http://hdl.handle.net/20.500.11937/35158 10.1016/j.jece.2016.04.030 Elsevuer BV restricted
repository_type	Digital Repository
institution_category	Local University
institution	Curtin University Malaysia
building	Curtin Institutional Repository
collection	Online Access
description	The search for effective and sustainable methods to degrade and remove recalcitrant dyes from textile effluents is a major research endeavor, owing to the escalating environmental and health concerns arising from the discharge of coloured effluents into water bodies. Plant-based peroxidases represent a reliable bio-resource for sustainable treatment of coloured effluents with the capacity to offer a continuous process for high throughput operation. The present study investigates the potential use of soybean peroxidase and Luffa acutangula (luffa) peroxidase, extracted from bio-wastes of soybean hulls and luffa skin peels respectively, for enzymatic degradation of azo dye methyl orange from liquid effluents. The enzymatic dye removal process was studied based on the consistent enzymatic activities of crude soybean peroxidase and luffa peroxidase extracts, which were 0.373 U mL-1 and 0.355 U mL-1 respectively. The effects of several process parameters including reaction time, pH, temperature, enzyme dosage, initial dye concentration and hydrogen peroxide concentration were investigated to optimise the performance of the enzymatic treatment process. Soybean peroxidase demonstrated a maximum dye decolourisation efficiency of 81.4% under the conditions of 1 h incubation at 30 °C using 2 mM of hydrogen peroxide, 0.5 mL crude soybean peroxidase and 30 mg L-1 methyl orange at pH 5.0. Also, luffa peroxidase yielded a maximum decolourisation efficiency of 75.3% under the conditions of 40 min at 40 °C using 2 mM hydrogen peroxide, 1.5 mL crude luffa peroxidase and 10 mg L-1 methyl orange at pH 3.0.
format	Journal Article
author	Chiong, T. Lau, John Lek, Z. Koh, B. Danquah, M.
spellingShingle	Chiong, T. Lau, John Lek, Z. Koh, B. Danquah, M. Enzymatic treatment of methyl orange dye in synthetic wastewater by plant-based peroxidase enzymes
author_facet	Chiong, T. Lau, John Lek, Z. Koh, B. Danquah, M.
author_sort	Chiong, T.
title	Enzymatic treatment of methyl orange dye in synthetic wastewater by plant-based peroxidase enzymes
title_short	Enzymatic treatment of methyl orange dye in synthetic wastewater by plant-based peroxidase enzymes
title_full	Enzymatic treatment of methyl orange dye in synthetic wastewater by plant-based peroxidase enzymes
title_fullStr	Enzymatic treatment of methyl orange dye in synthetic wastewater by plant-based peroxidase enzymes
title_full_unstemmed	Enzymatic treatment of methyl orange dye in synthetic wastewater by plant-based peroxidase enzymes
title_sort	enzymatic treatment of methyl orange dye in synthetic wastewater by plant-based peroxidase enzymes
publisher	Elsevuer BV
publishDate	2016
url	http://hdl.handle.net/20.500.11937/35158
first_indexed	2018-09-06T22:17:35Z
last_indexed	2018-09-06T22:17:35Z
_version_	1610898217171419136

Enzymatic treatment of methyl orange dye in synthetic wastewater by plant-based peroxidase enzymes

Similar Items