Genetic mapping of a major resistance gene to pea aphid (Acyrthosipon pisum) in the model legume Medicago truncatula

Resistance to the Australian pea aphid (PA; Acyrthosiphon pisum) biotype in cultivar Jester of the model legume Medicago truncatula is mediated by a single dominant gene and is phloem-mediated. The genetic map position for this resistance gene, APR (Acyrthosiphon pisum resistance), is provided and s...

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Main Authors:	Kamphuis, Lars, Guo, S., Gao, L., Singh, Karam
Format:	Journal Article
Published:	MDPI AG 2016
Online Access:	http://hdl.handle.net/20.500.11937/34278

id	curtin-20.500.11937-34278
recordtype	eprints
spelling	curtin-20.500.11937-342782017-09-13T15:10:08Z Genetic mapping of a major resistance gene to pea aphid (Acyrthosipon pisum) in the model legume Medicago truncatula Kamphuis, Lars Guo, S. Gao, L. Singh, Karam Resistance to the Australian pea aphid (PA; Acyrthosiphon pisum) biotype in cultivar Jester of the model legume Medicago truncatula is mediated by a single dominant gene and is phloem-mediated. The genetic map position for this resistance gene, APR (Acyrthosiphon pisum resistance), is provided and shows that APR maps 39 centiMorgans (cM) distal of the A. kondoi resistance (AKR) locus, which mediates resistance to a closely related species of the same genus bluegreen aphid (A. kondoi). The APR region on chromosome 3 is dense in classical nucleotide binding site leucine-rich repeats (NLRs) and overlaps with the region harbouring the RAP1 gene which confers resistance to a European PA biotype in the accession Jemalong A17. Further screening of a core collection of M. truncatula accessions identified seven lines with strong resistance to PA. Allelism experiments showed that the single dominant resistance to PA in M. truncatula accessions SA10481 and SA1516 are allelic to SA10733, the donor of the APR locus in cultivar Jester. While it remains unclear whether there are multiple PA resistance genes in an R-gene cluster or the resistance loci identified in the other M. truncatula accessions are allelic to APR, the introgression of APR into current M. truncatulacultivars will provide more durable resistance to PA. 2016 Journal Article http://hdl.handle.net/20.500.11937/34278 10.3390/ijms17081224 MDPI AG fulltext
repository_type	Digital Repository
institution_category	Local University
institution	Curtin University Malaysia
building	Curtin Institutional Repository
collection	Online Access
description	Resistance to the Australian pea aphid (PA; Acyrthosiphon pisum) biotype in cultivar Jester of the model legume Medicago truncatula is mediated by a single dominant gene and is phloem-mediated. The genetic map position for this resistance gene, APR (Acyrthosiphon pisum resistance), is provided and shows that APR maps 39 centiMorgans (cM) distal of the A. kondoi resistance (AKR) locus, which mediates resistance to a closely related species of the same genus bluegreen aphid (A. kondoi). The APR region on chromosome 3 is dense in classical nucleotide binding site leucine-rich repeats (NLRs) and overlaps with the region harbouring the RAP1 gene which confers resistance to a European PA biotype in the accession Jemalong A17. Further screening of a core collection of M. truncatula accessions identified seven lines with strong resistance to PA. Allelism experiments showed that the single dominant resistance to PA in M. truncatula accessions SA10481 and SA1516 are allelic to SA10733, the donor of the APR locus in cultivar Jester. While it remains unclear whether there are multiple PA resistance genes in an R-gene cluster or the resistance loci identified in the other M. truncatula accessions are allelic to APR, the introgression of APR into current M. truncatulacultivars will provide more durable resistance to PA.
format	Journal Article
author	Kamphuis, Lars Guo, S. Gao, L. Singh, Karam
spellingShingle	Kamphuis, Lars Guo, S. Gao, L. Singh, Karam Genetic mapping of a major resistance gene to pea aphid (Acyrthosipon pisum) in the model legume Medicago truncatula
author_facet	Kamphuis, Lars Guo, S. Gao, L. Singh, Karam
author_sort	Kamphuis, Lars
title	Genetic mapping of a major resistance gene to pea aphid (Acyrthosipon pisum) in the model legume Medicago truncatula
title_short	Genetic mapping of a major resistance gene to pea aphid (Acyrthosipon pisum) in the model legume Medicago truncatula
title_full	Genetic mapping of a major resistance gene to pea aphid (Acyrthosipon pisum) in the model legume Medicago truncatula
title_fullStr	Genetic mapping of a major resistance gene to pea aphid (Acyrthosipon pisum) in the model legume Medicago truncatula
title_full_unstemmed	Genetic mapping of a major resistance gene to pea aphid (Acyrthosipon pisum) in the model legume Medicago truncatula
title_sort	genetic mapping of a major resistance gene to pea aphid (acyrthosipon pisum) in the model legume medicago truncatula
publisher	MDPI AG
publishDate	2016
url	http://hdl.handle.net/20.500.11937/34278
first_indexed	2018-09-06T22:09:58Z
last_indexed	2018-09-06T22:09:58Z
_version_	1610897737919758336

Genetic mapping of a major resistance gene to pea aphid (Acyrthosipon pisum) in the model legume Medicago truncatula

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