Detection and discovery of novel arboviruses in Western Australia

Viruses from several recognized arthropod-borne virus families are regularly isolated in Australia. Many of these are important human and animal pathogens, and the emergence of novel or exotic arboviral pathogens pose additional threats. In Western Australia, arbovirus activity is monitored by the A...

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Main Authors:	Diviney, Sinead, Williams, David, Johansen, C., Quan, P., Briese, T., Lipkin, W., MacKenzie, John
Other Authors:	M. Cooley and S. Tristram
Format:	Conference Paper
Published:	Australian Society for Microbiology 2011
Online Access:	http://hdl.handle.net/20.500.11937/33324

id	curtin-20.500.11937-33324
recordtype	eprints
spelling	curtin-20.500.11937-333242017-01-30T13:36:23Z Detection and discovery of novel arboviruses in Western Australia Diviney, Sinead Williams, David Johansen, C. Quan, P. Briese, T. Lipkin, W. MacKenzie, John M. Cooley and S. Tristram Viruses from several recognized arthropod-borne virus families are regularly isolated in Australia. Many of these are important human and animal pathogens, and the emergence of novel or exotic arboviral pathogens pose additional threats. In Western Australia, arbovirus activity is monitored by the Arbovirus Surveillance and Research Laboratory. Viruses are isolated in cell culture from pools of mosquitoes, and their identification is then performed using a panel of monoclonal antibodies raised against medically important viruses belonging to the Alphavirus and Flavivirus genera in an enzyme immunoassay. A collection of arbovirus cultures that had evaded serological identification were further screened using molecular methods. A series of RT-PCR assays using primer sets targeting alphaviruses, flaviviruses and orbiviruses enabled identification of many of these cultures. The identity of the remaining isolates was subsequently determined using unbiased high-throughput sequencing. Novel members of the Rhabdoviridae (Oak Vale-like virus) and Reoviridae (Stretch Lagoon orbivirus) were identified. Viruses belonging to the Mapputta virus group of the Orthobunyaviridae family were also identified, as well as a single isolate of a novel reassortant virus, whose genome consists of a bunyamwera virus-like L segment and Batai virus-like M and S segments. The identification and characterisation of these viruses has informed subsequent molecular screening activities. These findings emphasise the importance of employing combinations of laboratory-based diagnostic and detection methods for comprehensive arbovirus surveillance. 2011 Conference Paper http://hdl.handle.net/20.500.11937/33324 Australian Society for Microbiology restricted
repository_type	Digital Repository
institution_category	Local University
institution	Curtin University Malaysia
building	Curtin Institutional Repository
collection	Online Access
description	Viruses from several recognized arthropod-borne virus families are regularly isolated in Australia. Many of these are important human and animal pathogens, and the emergence of novel or exotic arboviral pathogens pose additional threats. In Western Australia, arbovirus activity is monitored by the Arbovirus Surveillance and Research Laboratory. Viruses are isolated in cell culture from pools of mosquitoes, and their identification is then performed using a panel of monoclonal antibodies raised against medically important viruses belonging to the Alphavirus and Flavivirus genera in an enzyme immunoassay. A collection of arbovirus cultures that had evaded serological identification were further screened using molecular methods. A series of RT-PCR assays using primer sets targeting alphaviruses, flaviviruses and orbiviruses enabled identification of many of these cultures. The identity of the remaining isolates was subsequently determined using unbiased high-throughput sequencing. Novel members of the Rhabdoviridae (Oak Vale-like virus) and Reoviridae (Stretch Lagoon orbivirus) were identified. Viruses belonging to the Mapputta virus group of the Orthobunyaviridae family were also identified, as well as a single isolate of a novel reassortant virus, whose genome consists of a bunyamwera virus-like L segment and Batai virus-like M and S segments. The identification and characterisation of these viruses has informed subsequent molecular screening activities. These findings emphasise the importance of employing combinations of laboratory-based diagnostic and detection methods for comprehensive arbovirus surveillance.
author2	M. Cooley and S. Tristram
author_facet	M. Cooley and S. Tristram Diviney, Sinead Williams, David Johansen, C. Quan, P. Briese, T. Lipkin, W. MacKenzie, John
format	Conference Paper
author	Diviney, Sinead Williams, David Johansen, C. Quan, P. Briese, T. Lipkin, W. MacKenzie, John
spellingShingle	Diviney, Sinead Williams, David Johansen, C. Quan, P. Briese, T. Lipkin, W. MacKenzie, John Detection and discovery of novel arboviruses in Western Australia
author_sort	Diviney, Sinead
title	Detection and discovery of novel arboviruses in Western Australia
title_short	Detection and discovery of novel arboviruses in Western Australia
title_full	Detection and discovery of novel arboviruses in Western Australia
title_fullStr	Detection and discovery of novel arboviruses in Western Australia
title_full_unstemmed	Detection and discovery of novel arboviruses in Western Australia
title_sort	detection and discovery of novel arboviruses in western australia
publisher	Australian Society for Microbiology
publishDate	2011
url	http://hdl.handle.net/20.500.11937/33324
first_indexed	2018-09-06T22:01:42Z
last_indexed	2018-09-06T22:01:42Z
_version_	1610897217741127680

Detection and discovery of novel arboviruses in Western Australia

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