Characterization and genetic dissection of resistance to spotted alfalfa aphid (Therioaphis trifolii) in Medicago truncatula

Aphids cause significant yield losses in agricultural crops worldwide. Medicago truncatula, a model legume, cultivated pasture species in Australia and close relative of alfalfa (Medicago sativa), was used to study the defence response against Therioaphis trifolii f. maculate [spotted alfalfa aphid...

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Main Authors:	Kamphuis, L., Lichtenzveig, Judith, Peng, K., Guo, S., Klingler, J., Siddique, K., Gao, L., Singh, K.
Format:	Journal Article
Published:	Oxford University Press 2013
Subjects:	Sap-sucking insect Vein chlorosis Quantitative trait locus (QTL) Herbivory Antixenosis Antibiosis Phloem EPG
Online Access:	http://hdl.handle.net/20.500.11937/30734

id	curtin-20.500.11937-30734
recordtype	eprints
spelling	curtin-20.500.11937-307342017-09-13T15:09:36Z Characterization and genetic dissection of resistance to spotted alfalfa aphid (Therioaphis trifolii) in Medicago truncatula Kamphuis, L. Lichtenzveig, Judith Peng, K. Guo, S. Klingler, J. Siddique, K. Gao, L. Singh, K. Sap-sucking insect Vein chlorosis Quantitative trait locus (QTL) Herbivory Antixenosis Antibiosis Phloem EPG Aphids cause significant yield losses in agricultural crops worldwide. Medicago truncatula, a model legume, cultivated pasture species in Australia and close relative of alfalfa (Medicago sativa), was used to study the defence response against Therioaphis trifolii f. maculate [spotted alfalfa aphid (SAA)]. Aphid performance and plant damage were compared among three accessions. A20 is highly susceptible, A17 has moderate resistance, and Jester is strongly resistant. Subsequent analyses using A17 and A20, reciprocal F1s and an A17×A20 recombinant inbred line (RIL) population revealed that this moderate resistance is phloem mediated and involves antibiosis and tolerance but not antixenosis. Electrical penetration graph analysis also identified a novel waveform termed extended potential drop, which occurred following SAA infestation of M. truncatula. Genetic dissection using the RIL population revealed three quantitative trait loci on chromosomes 3, 6, and 7 involved in distinct modes of aphid defence including antibiosis and tolerance. An antibiosis locus resides on linkage group 3 (LG3) and is derived from A17, whereas a plant tolerance and antibiosis locus resides on LG6 and is derived from A20, which exhibits strong temporary tolerance. The loci identified reside in regions harbouring classical resistance genes, and introgression of these loci in current medic cultivars may help provide durable resistance to SAA, while elucidation of their molecular mechanisms may provide valuable insight into other aphid-plant interactions. 2013 Journal Article http://hdl.handle.net/20.500.11937/30734 10.1093/jxb/ert305 Oxford University Press fulltext
repository_type	Digital Repository
institution_category	Local University
institution	Curtin University Malaysia
building	Curtin Institutional Repository
collection	Online Access
topic	Sap-sucking insect Vein chlorosis Quantitative trait locus (QTL) Herbivory Antixenosis Antibiosis Phloem EPG
spellingShingle	Sap-sucking insect Vein chlorosis Quantitative trait locus (QTL) Herbivory Antixenosis Antibiosis Phloem EPG Kamphuis, L. Lichtenzveig, Judith Peng, K. Guo, S. Klingler, J. Siddique, K. Gao, L. Singh, K. Characterization and genetic dissection of resistance to spotted alfalfa aphid (Therioaphis trifolii) in Medicago truncatula
description	Aphids cause significant yield losses in agricultural crops worldwide. Medicago truncatula, a model legume, cultivated pasture species in Australia and close relative of alfalfa (Medicago sativa), was used to study the defence response against Therioaphis trifolii f. maculate [spotted alfalfa aphid (SAA)]. Aphid performance and plant damage were compared among three accessions. A20 is highly susceptible, A17 has moderate resistance, and Jester is strongly resistant. Subsequent analyses using A17 and A20, reciprocal F1s and an A17×A20 recombinant inbred line (RIL) population revealed that this moderate resistance is phloem mediated and involves antibiosis and tolerance but not antixenosis. Electrical penetration graph analysis also identified a novel waveform termed extended potential drop, which occurred following SAA infestation of M. truncatula. Genetic dissection using the RIL population revealed three quantitative trait loci on chromosomes 3, 6, and 7 involved in distinct modes of aphid defence including antibiosis and tolerance. An antibiosis locus resides on linkage group 3 (LG3) and is derived from A17, whereas a plant tolerance and antibiosis locus resides on LG6 and is derived from A20, which exhibits strong temporary tolerance. The loci identified reside in regions harbouring classical resistance genes, and introgression of these loci in current medic cultivars may help provide durable resistance to SAA, while elucidation of their molecular mechanisms may provide valuable insight into other aphid-plant interactions.
format	Journal Article
author	Kamphuis, L. Lichtenzveig, Judith Peng, K. Guo, S. Klingler, J. Siddique, K. Gao, L. Singh, K.
author_facet	Kamphuis, L. Lichtenzveig, Judith Peng, K. Guo, S. Klingler, J. Siddique, K. Gao, L. Singh, K.
author_sort	Kamphuis, L.
title	Characterization and genetic dissection of resistance to spotted alfalfa aphid (Therioaphis trifolii) in Medicago truncatula
title_short	Characterization and genetic dissection of resistance to spotted alfalfa aphid (Therioaphis trifolii) in Medicago truncatula
title_full	Characterization and genetic dissection of resistance to spotted alfalfa aphid (Therioaphis trifolii) in Medicago truncatula
title_fullStr	Characterization and genetic dissection of resistance to spotted alfalfa aphid (Therioaphis trifolii) in Medicago truncatula
title_full_unstemmed	Characterization and genetic dissection of resistance to spotted alfalfa aphid (Therioaphis trifolii) in Medicago truncatula
title_sort	characterization and genetic dissection of resistance to spotted alfalfa aphid (therioaphis trifolii) in medicago truncatula
publisher	Oxford University Press
publishDate	2013
url	http://hdl.handle.net/20.500.11937/30734
first_indexed	2018-09-06T21:39:18Z
last_indexed	2018-09-06T21:39:18Z
_version_	1610895807808012288

Characterization and genetic dissection of resistance to spotted alfalfa aphid (Therioaphis trifolii) in Medicago truncatula

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