Identification and characterization of resistance to cowpea aphid (Aphis craccivora Koch) in Medicago truncatula

Background: Cowpea aphid (CPA; Aphis craccivora) is the most important insect pest of cowpea and also causes significant yield losses in other legume crops including alfalfa, beans, chickpea, lentils, lupins and peanuts. In many of these crops there is no natural genetic resistance to this sap-sucki...

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Main Authors:	Kamphuis, L., Gao, L., Singh, Karambir
Format:	Journal Article
Published:	BioMed Central Ltd 2012
Online Access:	http://hdl.handle.net/20.500.11937/26334

id	curtin-20.500.11937-26334
recordtype	eprints
spelling	curtin-20.500.11937-263342017-09-13T15:26:50Z Identification and characterization of resistance to cowpea aphid (Aphis craccivora Koch) in Medicago truncatula Kamphuis, L. Gao, L. Singh, Karambir Background: Cowpea aphid (CPA; Aphis craccivora) is the most important insect pest of cowpea and also causes significant yield losses in other legume crops including alfalfa, beans, chickpea, lentils, lupins and peanuts. In many of these crops there is no natural genetic resistance to this sap-sucking insect or resistance genes have been overcome by newly emerged CPA biotypes. Results: In this study, we screened a subset of the Medicago truncatula core collection of the South Australian Research and Development Institute (SARDI) and identified strong resistance to CPA in a M. truncatula accession SA30199, compared to all other M. truncatula accessions tested. The biology of resistance to CPA in SA30199 plants was characterised compared to the highly susceptible accession Borung and showed that resistance occurred at the level of the phloem, required an intact plant and involved a combination of antixenosis and antibiosis. Quantitative trait loci (QTL) analysis using a F 2 population (n = 150) from a cross between SA30199 and Borung revealed that resistance to CPA is controlled in part by a major quantitative trait locus (QTL) on chromosome 2, explaining 39% of the antibiosis resistance. Conclusions: The identification of strong CPA resistance in M. truncatula allows for the identification of key regulators and genes important in this model legume to give effective CPA resistance that may have relevance for other legume crops. The identified locus will also facilitate marker assisted breeding of M. truncatula for increased resistance to CPA and potentially other closely related Medicago species such as alfalfa. 2012 Journal Article http://hdl.handle.net/20.500.11937/26334 10.1186/1471-2229-12-101 BioMed Central Ltd unknown
repository_type	Digital Repository
institution_category	Local University
institution	Curtin University Malaysia
building	Curtin Institutional Repository
collection	Online Access
description	Background: Cowpea aphid (CPA; Aphis craccivora) is the most important insect pest of cowpea and also causes significant yield losses in other legume crops including alfalfa, beans, chickpea, lentils, lupins and peanuts. In many of these crops there is no natural genetic resistance to this sap-sucking insect or resistance genes have been overcome by newly emerged CPA biotypes. Results: In this study, we screened a subset of the Medicago truncatula core collection of the South Australian Research and Development Institute (SARDI) and identified strong resistance to CPA in a M. truncatula accession SA30199, compared to all other M. truncatula accessions tested. The biology of resistance to CPA in SA30199 plants was characterised compared to the highly susceptible accession Borung and showed that resistance occurred at the level of the phloem, required an intact plant and involved a combination of antixenosis and antibiosis. Quantitative trait loci (QTL) analysis using a F 2 population (n = 150) from a cross between SA30199 and Borung revealed that resistance to CPA is controlled in part by a major quantitative trait locus (QTL) on chromosome 2, explaining 39% of the antibiosis resistance. Conclusions: The identification of strong CPA resistance in M. truncatula allows for the identification of key regulators and genes important in this model legume to give effective CPA resistance that may have relevance for other legume crops. The identified locus will also facilitate marker assisted breeding of M. truncatula for increased resistance to CPA and potentially other closely related Medicago species such as alfalfa.
format	Journal Article
author	Kamphuis, L. Gao, L. Singh, Karambir
spellingShingle	Kamphuis, L. Gao, L. Singh, Karambir Identification and characterization of resistance to cowpea aphid (Aphis craccivora Koch) in Medicago truncatula
author_facet	Kamphuis, L. Gao, L. Singh, Karambir
author_sort	Kamphuis, L.
title	Identification and characterization of resistance to cowpea aphid (Aphis craccivora Koch) in Medicago truncatula
title_short	Identification and characterization of resistance to cowpea aphid (Aphis craccivora Koch) in Medicago truncatula
title_full	Identification and characterization of resistance to cowpea aphid (Aphis craccivora Koch) in Medicago truncatula
title_fullStr	Identification and characterization of resistance to cowpea aphid (Aphis craccivora Koch) in Medicago truncatula
title_full_unstemmed	Identification and characterization of resistance to cowpea aphid (Aphis craccivora Koch) in Medicago truncatula
title_sort	identification and characterization of resistance to cowpea aphid (aphis craccivora koch) in medicago truncatula
publisher	BioMed Central Ltd
publishDate	2012
url	http://hdl.handle.net/20.500.11937/26334
first_indexed	2018-09-06T21:01:13Z
last_indexed	2018-09-06T21:01:13Z
_version_	1610893411849601024

Identification and characterization of resistance to cowpea aphid (Aphis craccivora Koch) in Medicago truncatula

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