Introductory microbiology lab skills and techniques in food science - Shen, Gangqiang; Zhang, Yifan (Oxford,United Kingdom : Elsevier Science, 2022)
1. Introduction of microbial and chemical safety and lab notebook record -- 2. Practice of bright-field microscope -- 3. Preparation of smears and simple stain practice -- 4. Gram-stain practice -- 5. Acid-fast stain practice -- 6. Endospore stain practice -- 7. Aseptic technique-bacteria transfer and streak plating -- 8. Lab practice test 1-identify unknown bacteria using Gram-stain -- 9. Introduction of bacteria medium, nutritional requirements (synthetic and complex media), selective & differential media (MacConkey, mannitol salt, blood agar) -- 10. Lab practice test 2- dilution technology and quiz -- 11. Numeration bacteria population by pour plating --12. Numeration bacteria population by spread plating -- 13. External conditions for bacterial growth (temperature, pH, oxygen, osmotic) -- 14. Cultivation of anaerobic bacteria -- 15. Biochemistry test of bacteria-1 (urease test, carbohydrate fermentation, catalase test, oxidase test)
Includes bibliographical references and index (pages 155-158)
Keywords: Food microbiology -- Laboratory manuals; Food science -- Techniques