rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Cytotoxic mechanism study of damnacanthal and nordamnacanthal of morinda citrifolia roots in vitro
  name:
    name part: Wan Norsalehah Wan Abdullah
    role:
      role term: creator
    role:
      role term: author
  name:
    name part: Universiti Sultan Zainal Abidin
    name part: Faculty of Bioresources and Food Industry
  type of resource: text
  genre: Dissertations, Academic
  origin info:
    place:
      place term: my
    date issued: 2017
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xiv, 132 leaves : illustrations (some colour) ; 30 cm.
  abstract: Natural products have been considered as valuable sources for anticancer drug discovery. Damncanthal (DAM) and nordamnacanthal (NDAM), are two anthraquinones derived from roots of Morinda cilrifolia (family Rubiaceae). The purpose of this study is to evaluate the cytotoxic mechanism of DAM and NDAM in vitro. The cytotoxic effects of DAM and NDAM, were investigated against several cancerous cell lines such as CEM-SS), WEI-II-3B, HT29, MCF-7, DBTRG, Hel,a, and HL-60 cells. The percentage of cell viability was determined using a microtetrazolium assay (MTT). I-I L-60 was observed to be the most sensitive cell line towards DAM and NDAM with CDso values of 10 ug/ml and 11.5 ug/ml for DAM and NDAM, respectively. Meanwhile the CDso value for WEHI-3B cells treated with DAM and NDAM was 24 ug/rnl and 27 ug/ml, respectively. On the other hand, the viability of normal peripheral blood mononuclear cells (PBMC) was not affected. Both compounds also exhibited time-dependent inhibitory effects of the cell line growth, evidenced by the reduction in cell viability and number of viable cells as determined from the trypan blue exclusion assay. From the flow cytometric analysis on the cell cycle of both cell types, it could be observed that both compounds induced apoptosis in WEHI-3B and HL-60 cells, respectively. The percentage of sub-G 1 population of I-IL-60 cells increased from 2.5% to 19. I % after treatment with DAM and from 2.5% to 18.6% after treatment with NDAM. These compounds also induced cell cycle arrest in S-phase for HL-60 and WEHI-3B cells. The microscope analysis on the treated HL-60 and WEHI- 3B, including light microscopy, showed that these compounds induced two types of cell death: apoptosis and necrosis. Fluorescence microscopy following staining with low concentration of acridine orange and propidium iodide (AO/PI) were used to visualize morphological changes of viable, apoptotic, or necrotic cells simultaneously. The early apoptotic stages of WEI-II-3B and HL-60 …
  table of contents: 1. Introduction -- 2. Literature review -- 3. Material and methods -- 4. Results -- 5. Discussion -- 6. Conclusions
  note: Wan Norsalehah binti Wan Abdullah
  note: Thesis (Degree of Master of Science) - Universiti Sultan Zainal Abidin, 2017
  note: Includes bibliographical references (p. 116-131)
  subject:
    name:
      name part: Universiti Sultan Zainal Abidin
    genre: Faculty of Bioresources and Food Industry
    topic: Dissertations
  subject:
    name:
      name part: Universiti Sultan Zainal Abidin
    topic: Dissertations
  subject:
    topic: Aquatic biology
  subject:
    topic: Biotechnology
  subject:
    topic: Chemistry
  classification: TP248.13 .W36 2017
  record info:
    record content source: UniSZA
    record creation date: 180702
    record change date: 20200723093000.0
    record identifier: 0000094525