rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Goniothalamin as anti-leukemic agent in WEHI-3B myelomonocyte leukaemia animal model in vitro and in vivo
  name:
    name part: Rola Ali Saeed Alshargaby
    role:
      role term: creator
    role:
      role term: author
  name:
    name part: Universiti Sultan Zainal Abidin
    name part: Faculty of Bioresources & Food Industry
  type of resource: text
  genre: Dissertations, Academic
  origin info:
    place:
      place term: my
    date issued: 2014
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xiv,150 leaves : ill. (some col.) ; 30 cm.
  abstract: Goniothalamin (GTN) is a styryl-lactone compound, which was extracted and isolated from Goniothalamus macrophyllus belonging to the Annonacae family. Goniothalamin was reported to be cytotoxic towards several tumor cell lines such as pancreas carcinoma, gastric carcinoma, breast carcinoma and leukemia cell lines. In this study, the in vitro cytotoxic activity toward Mouse myelomoncytic leukemia cell line (WEHI 3B) and in vivo anti leukemic activity of GTN were carried out. The Cytotoxic effects of fifty percent (IC50) for GTN-treated WEHI 3B cells were determined using microtetrazolium (MTT) assay; it was 1.7±O.2 ug/ml, The compound had no effect on the normal peripheral blood lymphocyte and normal mouse fibroblast cell line (3T3) at the same concentration used in the leukemia cell lines. The cell proliferation rate of GTN treated WEHI 3B leukemia cell line was reduced significantly in a dose and time dependent manner as compared to the untreated control. Treatment of WEHI 3B cells with IC50 and IC25 concentrations of GTN caused a reduction of the cell viability in the dose and time dependent manner. Moreover, MTT Proliferation assay showed that the growth rates for GTN-treated WEHI 3B cells decreased in the dose and time dependent manner. It was confirmed that the mode of cell death in response to GTN treatment on WEHI 3B cell line was by apoptosis. Morphological features of apoptosis were observed by the Phase Contrast Microscopy and Fluorescence Microscopy (Acridine Orange (AO) and Propidium Iodide (PI) staining). The features observed included chromatin condensation and margination along the inner nuclear membrane, cytoplasmic condensation, and membrane blebbing without disintegration of the cellular membrane. These were further confirmed with DNA laddering in the agarose gel electrophoresis assay. However, analysis of the cellular DNA content using PI showed that GTN caused an increase in sub-Gl region (apoptosis peaks). Early apoptosis was observed by annexin …
  table of contents: 1. Introduction -- 2. Literature review -- 3. Material and method -- 4. Results -- 5. Discussion -- 6. Conclusion and recommendations
  note: Rola Ali Saeed Alshargaby
  note: Thesis (Degree of Doctor of Philosophy) - Universiti Sultan Zainal Abidin, 2014
  note: Includes bibliographical references (leaves 115-126)
  subject:
    name:
      name part: Cytotoxicity, Immunologic
  subject:
    name:
      name part: Universiti Sultan Zainal Abidin
    genre: Faculty of Bioresources and Food Industry
    topic: Dissertations
  subject:
    name:
      name part: Universiti Sultan Zainal Abidin
    topic: Dissertations
  classification: RC271 .S53 2014
  record info:
    record content source: UniSZA
    record creation date: 170111
    record change date: 20200723093000.0
    record identifier: 0000090325