| Summary: | Aloe-emodin is a biologically active anthraquinone that isolated from the
flowers of Cassia a/ata belonging to the Caesalpiniae subfamily. This plant
traditionally used to treat ringworms throughout the world and found wildly
in tropical countries. The purpose of this study was to examine the in vitro
cytotoxicity and genotoxicity effect of aloe-emodin on human
promyelocytic leukemia cells (HL-60). For cytotoxicity assessment, there
were several experiments done such as MTT assay, double staining
AO/PI, DNA fragmentation, proliferation and annexin-V FITC. Meanwhile
for genetoxicity assessment, alkaline comet assay was done by analysed
each single cells after treated with aloe-emodin. For early screening
purpose, MTT assay was used to determine the cytotoxic dose of several
compounds on cancerous cell lines and normal cell line. Among the
cancer cells lines, aloe-emodin showed strong killing effects against HL-60
cells. In contrast, aloe-emodin did have a mild cytotoxic effect towards
mouse fibroblast NIH/3T3 cells. Thus, the HL-60 cells only were used to
determine the mode of cell death after treated with aloe-emodin. The
mode of cell death was mainly apoptosis assessed by acridine
orange/propidium iodide (AO/PI) double staining method and Annexin VFITC/
PI assays. Apoptotic cells characteristics such as the presence of
bleb bing cells and forming of apoptotic bodies were observed after HL-60
cells treated with aloe-emodin
|
|---|