Scanning electron microscopy for the life sciences

"Recent developments in scanning electron microscopy (SEM) have resulted in a wealth of new applications for cell and molecular biology, as well as related biological disciplines. It is now possible to analyze macromolecular complexes within their three-dimensional cellular microenvironment in...

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Bibliographic Details
Main Author: Schatten, Heide (Author)
Format: Book
Language:English
Published: Cambridge, England : Cambridge University Press , c2013
Series:Advances in microscopy and microanalysis
Subjects:

MARC

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020 |a 0521195993 (hardback : alk. paper) 
020 |a 9780521195997 (hardback : alk. paper) 
050 0 0 |a QH324.9.X2   |b S33 2013 
090 0 0 |a QH324.9.X2   |b S33 2013 
245 0 0 |a Scanning electron microscopy for the life sciences   |c [edited by] Heide Schatten 
260 |a Cambridge, England :   |b Cambridge University Press ,   |c c2013 
300 |a xi, 261 p. :   |b ill. (some col.) ;   |c 25 cm. 
490 1 |a Advances in microscopy and microanalysis 
504 |a Includes bibliographical references and index 
505 0 |a 1. The role of scanning electron microscopy in cell and molecular biology: SEM basics, past accomplishments and new frontiers -- 2. Corrosion casting technique -- 3. Revealing the internal structure of cells in three dimensions with scanning electron microscopy -- 4. Mitochondria form continuous intracellular network-structures visualized with high-resolution field-emission scanning electron microscopy -- 5. Is the scanning mode the future of electron microscopy in cell biology? -- 6. High resolution labeling for correlative microscopy -- 7. The use of SEM to explore virus structure and trafficking -- 8. High resolution scanning electron microscopy of the nuclear surface in Herpes Simplex Virus 1 infected cells -- 9. Scanning electron microscopy of chromosomes: structural and analytical investigations -- 10. A method to visualize the microarchitecture of glycoprotein matrices with scanning electron microscopy -- 11. Scanning electron microscopy of cerebellar intrinsic circuits -- 12. Application of in vivo cryotechnique to living animal organs examined by scanning electron microscopy -- 13. SEM in dental research -- 14. SEM, teeth and palaeoanthropology: the secret of ancient human diets 
520 |a "Recent developments in scanning electron microscopy (SEM) have resulted in a wealth of new applications for cell and molecular biology, as well as related biological disciplines. It is now possible to analyze macromolecular complexes within their three-dimensional cellular microenvironment in near native states at high resolution, and to identify specific molecules and their structural and molecular interactions. New approaches include cryo-SEM applications and environmental SEM (ESEM), staining techniques and processing applications combining embedding and resin-extraction for imaging with high resolution SEM, and advances in immuno-labeling. New developments include helium ion microscopy, automated block-face imaging combined with serial sectioning inside an SEM chamber, and Focused Ion Beam Milling (FIB) combined with block-face SEM. With chapters written by experts, this guide gives an overview of SEM and sample processing for SEM, and highlights several advances in cell and molecular biology that greatly benefited from using conventional, cryo, immuno, and high-resolution SEM."--P. 4 of cover 
650 0 |a Biology   |x Methodology 
650 0 |a Scanning electron microscopy 
700 1 |a Schatten, Heide ,   |e author 
999 |a 1000159034   |b Book   |c OPEN SHELF (30 DAYS)   |e Tembila Campus