Culture of animal cells : a manual of basic technique and specialized applications
* This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. * Updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Hoboken, New Jersey :
Wiley-Blackwell ,
c2010
|
| Edition: | 6th ed |
| Subjects: |
Table of Contents:
- 1. Introduction
- 2. Biology of cultured cells
- 3. Laboratory design, layout, and equipment
- 4. Equipment and materials
- 5. Aseptic technique
- 6. Safety, bioethics, and validation
- 7. Culture vessels and substrates
- 8. Defined media and supplements
- 9. Serum-free media
- 10. Preparation and sterilization
- 11. Primary culture
- 12. Subculture and cell lines
- 13. Cloning and selection
- 14. Cell separation
- 15. Characterization
- 16. Differentiation
- 17. Transformation and immortalization
- 18. Contamination
- 19. Cryopreservation
- 20. Quantitation
- 21. Cytotoxicity
- 22. Specialized cells
- 23. Stem cells, germ cells, and amniocytes
- 24. Culture of tumor cells
- 25. Three-dimensional culture
- 26. Scale-up and automation
- 27. Specialized techniques
- 28. Training programs
- 29. Problem solving
- 30. In conclusion