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110519s2010 nju eng |
| 020 |
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|a 0470528125 (cloth : alk. paper)
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| 020 |
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|a 9780470528129 (cloth : alk. paper)
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| 050 |
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|a QH585.2
|b .F74 2010
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| 090 |
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|a QH585.2
|b .F74 2010
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| 100 |
1 |
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|a Freshney, R. Ian ,
|e author
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| 245 |
1 |
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|a Culture of animal cells :
|b a manual of basic technique and specialized applications
|c R. Ian Freshney
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| 250 |
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|a 6th ed
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| 260 |
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|a Hoboken, New Jersey :
|b Wiley-Blackwell ,
|c c2010
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| 300 |
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|a xxxi, 732 p., [28] p. of plates :
|b ill. (some col.) ;
|c 29 cm.
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| 504 |
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|a Includes bibliographical references and index
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| 505 |
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|a 1. Introduction -- 2. Biology of cultured cells -- 3. Laboratory design, layout, and equipment -- 4. Equipment and materials -- 5. Aseptic technique -- 6. Safety, bioethics, and validation -- 7. Culture vessels and substrates -- 8. Defined media and supplements -- 9. Serum-free media -- 10. Preparation and sterilization -- 11. Primary culture -- 12. Subculture and cell lines -- 13. Cloning and selection -- 14. Cell separation -- 15. Characterization -- 16. Differentiation -- 17. Transformation and immortalization -- 18. Contamination -- 19. Cryopreservation -- 20. Quantitation -- 21. Cytotoxicity -- 22. Specialized cells -- 23. Stem cells, germ cells, and amniocytes -- 24. Culture of tumor cells -- 25. Three-dimensional culture -- 26. Scale-up and automation -- 27. Specialized techniques -- 28. Training programs -- 29. Problem solving -- 30. In conclusion
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| 520 |
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|a * This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. * Updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer
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| 650 |
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|a Cell culture
|v Laboratory manuals
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| 650 |
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|a Tissue culture
|v Laboratory manuals
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| 999 |
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|a 1000139669
|b Book
|c OPEN SHELF (30 DAYS)
|e Tembila Campus
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