FLIM microscopy in biology and medicine

Bibliographic Details
Main Authors: Clegg, Robert M. (Author), Periasamy, Ammasi (Author)
Format: Book
Published: Boca Raton : Taylor & Francis , c2010
Subjects:
Table of Contents:
  • 1. Fluorescence lifetime-resolved imaging; what, why, who-a prologue
  • 2. Principles of fluorescence for quqntitative fluorescence microscopy
  • 3. Visible fluorescent protiens for FRET-FLIM
  • 4. Wide-field fluorescence lifetime imaging microscopy using a gated image intensifier camera
  • 5. Frequency-domain FLIM
  • 6. Laser scanning confocal FLIM microscopy
  • 7. Multiphoton fluorescence lifetime imaging at the dawn of clinical application
  • 8. FLIM microscopy with a streak camera: monitoring metabolic process in living cells and tissues
  • 9. Spectrally resolved fluorescence lifetime imaging microscopy: SLIM/mwFLIM
  • 10. Time-resolved fluorescence anisotropy
  • 11. General concerns of FLIM data representation and analysis: frequency-domain model-free analysis
  • 12. Nonlinear curve-fitting methods for time-resolved data analysis
  • 13. Global analysis of frequency domain FLIM data
  • 14. FLIM applications in the biomedical sciences