Cell-based assays for high-throughput screening : methods and protocols

Bibliographic Details
Main Authors:	Clemons, Paul A. (Author), Tolliday, Nicola J. (Author), Wagner, Bridget K. (Author)
Format:	Book
Language:	English
Published:	New York : Humana , c2009
Series:	Methods in molecular biology v. 486 Springer protocols
Subjects:	Biological assay High throughput screening (Drug development) Drug Discovery Drug Evaluation, Preclinical Laboratory Manuals

Table of Contents:

1. Introduction: cell-based assays for high-throughput screening
2. High-throughput screening of model bacteria
3. Cell-based assays to probe teh ERK MAP kinase
4. Large-scale small-molecule screen using Zebrafish Embryos
5. Whole-animal high-throughput screens: the C. elegans model
6. Whole-organism screening: plants
7. Fluorescence-based assays
8. Reporter gene assays
9. Screening for chemical inhibitors of heterologous proteins expressed in yeast using a simple growth-restoration assay
10. Assay for isolution of inhibitorsof Her2-kinase expression
11. High-content screening: flow cytometry analysis
12. High-throughput real-time PCR for detection
13. Interpretation of uniform-well readouts
14. Extracting rich information from images

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