Basic methods in molecular biology

Bibliographic Details
Main Authors:	Davis, Leonard G. (Author), Battey, James F. (Author), Kuehl, W. Michael (Author)
Format:	Book
Language:	English
Published:	Norwalk, CT : Appleton & Lange , c1994
Edition:	2nd ed
Subjects:	Molecular biology > Methodology

Table of Contents:

1. General methods
2. Bacterial strains and cloning vectors
3. Enzymes that modify DNA and RNA
4. In vitro amplification of DNA using the polymerase chain reaction (PCR) and the thermostable Taq DNA polymerase
5. DNA restriction fragment analysis and preparation
6. In vitro labeling of probes and filter hybridization
7. Plasmid DNA preparation from E coli hosts
8. Preparation of DNA from lambda bacteriophage clones
9. Subcloning fragments into plasmid vectors and plasmid construction
10. Preparation of genomic DNA
11. Preparation and analysis of RNA from eukaryotic cells
12. Genomic cloning
13. Preparation and analysis of cDNA libraries
14. Preparation of subtractive cDNA
15. Chain termination sequencing
16. Transfection of mammalian cells
17. Basic methods of protein analysis
18. In situ hybridization
19. Transgenic mouse preparation
20. Detection and in vitro generation of specific mutations in genes and cDNAs
App. I. Preparation of stock solutions
App. II. Generally useful information
App. III. Suppliers of reagents and equipment

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