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20160626093000.0 |
| 008 |
080309s1994 ctu eng |
| 020 |
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|a 0838506429
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| 090 |
0 |
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|a QH506
|b .D39 1994
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| 100 |
1 |
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|a Davis, Leonard G. ,
|e author
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| 245 |
1 |
0 |
|a Basic methods in molecular biology
|c Leonard G. Davis, W. Michael Kuehl, James F. Battey
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| 250 |
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|a 2nd ed
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| 260 |
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|a Norwalk, CT :
|b Appleton & Lange ,
|c c1994
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| 300 |
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|a xiv, 777 p. :
|b ill. ;
|c 26 cm.
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| 504 |
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|a Includes bibliographical references
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| 505 |
0 |
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|a 1. General methods -- 2. Bacterial strains and cloning vectors -- 3. Enzymes that modify DNA and RNA -- 4. In vitro amplification of DNA using the polymerase chain reaction (PCR) and the thermostable Taq DNA polymerase -- 5. DNA restriction fragment analysis and preparation -- 6. In vitro labeling of probes and filter hybridization -- 7. Plasmid DNA preparation from E coli hosts -- 8. Preparation of DNA from lambda bacteriophage clones -- 9. Subcloning fragments into plasmid vectors and plasmid construction -- 10. Preparation of genomic DNA -- 11. Preparation and analysis of RNA from eukaryotic cells -- 12. Genomic cloning -- 13. Preparation and analysis of cDNA libraries -- 14. Preparation of subtractive cDNA -- 15. Chain termination sequencing -- 16. Transfection of mammalian cells -- 17. Basic methods of protein analysis -- 18. In situ hybridization -- 19. Transgenic mouse preparation -- 20. Detection and in vitro generation of specific mutations in genes and cDNAs -- App. I. Preparation of stock solutions -- App. II. Generally useful information -- App. III. Suppliers of reagents and equipment
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| 650 |
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0 |
|a Molecular biology
|x Methodology
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| 700 |
1 |
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|a Battey, James F. ,
|e author
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| 700 |
1 |
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|a Kuehl, W. Michael ,
|e author
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| 999 |
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|a 1000119407
|b Book
|c OPEN SHELF (30 DAYS)
|e Tembila Campus
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