Immunochemistry in practice

Bibliographic Details
Main Authors: Johnstone, Alan (Author), Thorpe, Robin (Author)
Format: Book
Language:English
Published: Cambridge, Mass : Blackwell Science , c1996
Edition:3rd ed
Subjects:
Table of Contents:
  • 1. Basic techniques
  • 2. Production of antibodies
  • 3. Purification of immunoglobulins, constituent chains and fragments
  • 4. Isolation and fractionation of lymphocytes
  • 5. Radiolabelling techniques
  • 6. Precipitation techniques in agar and agarose
  • 7. Analytical electrophoresis and isoelectric focusing
  • 8. Specific detection of antigens separated by polyacrylamide gel electrophoresis
  • 9. Preparative gel techniques
  • 10. Affinity chromatography and immunoprecipitation
  • 11. Coupling antibodies to fluorochromes, enzymes and biotin
  • 12. Immunoassays
  • 13. Immunocytochemistry, immunohistochemistry and flow cytofluorimetry