Animal cell biotechnology : methods and protocols
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press ,
2007
|
| Edition: | 2nd ed |
| Series: | Methods in biotechnology
24 |
| Subjects: | |
| Online Access: | Table of contents only Publisher description |
Table of Contents:
- Part I. Basic Techniques for Establishing Production Cell Lines
- 1. Transduction Technologies / David A. Fluri and Martin Fussenegger
- 2. Cells for Gene Therapy and Vector Production / Christophe Delenda, Miguel Chillon, Anne-Marie Douar and Otto-Wilhelm Merten
- 3. Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments / Alexandra Dorn-Beineke, Stefanie Nittka and Michael Neumaier
- 4. DNA Fingerprinting and Characterization of Animal Cell Lines / Glyn N. Stacey, Ed Byrne and J. Ross Hawkins
- Part II. Basic Cultivation Techniques
- 5. Microcarrier Cell Culture Technology / Gerald Bluml
- 6. Cell Encapsulation / Holger Hubner
- 7. Tools for High-Throughput Medium and Process Optimization / Martin Jordan and Nigel Jenkins
- Part III. Cell Characterization and Analysis
- 8. Cell Counting and Viability Measurements / Michael Butler and Maureen Spearman
- 9. Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry / Silvia Carroll, Mariam Naeiri and Mohamed Al-Rubeai
- 10. Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors / Andre A. Neves and Kevin M. Brindle
- 11. Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture / Heino Buntemeyer
- 12. Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation / Derek Adams, Rashmi Korke and Wei-Shou Hu
- 13. Measurement of Apoptosis in Cell Culture / Adiba Ishaque and Mohamed Al-Rubeai
- 14. Metabolic Flux Estimation in Mammalian Cell Cultures / Chetan T. Goudar, Richard Biener, James M. Piret and Konstantin B. Konstantinov
- Part IV. Special Cultivation Techniques
- 15. Disposable Bioreactors for Inoculum Production and Protein Expression / Regine Eibl and Dieter Eibl
- 16. Hollow Fiber Cell Culture / John M. Davis
- 17. Cultivation of Mammalian Cells in Fixed-Bed Reactors / Ralf Portner and Oscar B. J. Platas Barradas
- 18. Configuration of Bioreactors / Dirk E. Martens and Evert Jan van den End
- Part V. Downstream Techniques
- 19. Membrane Filtration in Animal Cell Culture / Peter Czermak, Dirk Nehring and Ranil Wickramasinghe
- 20. Chromatographic Techniques in the Downstream Processing of (Recombinant) Proteins / Ruth Freitag
- Part VI. Special Applications
- 21. Vaccine Production: State of The Art and Future Needs in Upstream Processing / Yvonne Genzel and Udo Reichl
- 22. Retrovirus Production and Characterization / Pedro E. Cruz, Marlene Carmo, Teresa Rodrigues and Paula Alves
- 23. Insect Cell Cultivation and Generation of Recombinant Baculovirus Particles for Recombinant Protein Production / Sabine Geisse.