Culture of animal cells : a manual of basic technique
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Hoboken, NJ :
Wiley-Liss ,
c2005
|
| Edition: | 5th ed |
| Subjects: |
Table of Contents:
- 1. Introduction
- 2. Training programs
- 3. Biology of cultured cells
- 4. Laboratory design and layout
- 5. Equipment
- 6. Aseptic technique
- 7. Safety, bioethics, and validation
- 8. Culture vessels and substrates
- 9. Defined media and supplements
- 10. Serum-free media
- 11. Preparation and sterilization
- 12. Primary culture
- 13. Subculture and cell lines
- 14. Cloning and selection
- 15. Cell separation
- 16. Characterization
- 17. Differentiation
- 18. Transformation and immortalization
- 19. Contamination
- 20. Cryopreservation
- 21. Quantitation
- 22. Cytotoxicity
- 23. Culture of specific cell types
- 24. Culture of tumor cells
- 25. Organotypic culture
- 26. Scale-up
- 27. Specilized techniques
- 28. Problem solving
- 29. Conclusion