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| LEADER |
00000cam a2200000 7i4500 |
| 001 |
0000054936 |
| 005 |
20210208093000.0 |
| 008 |
080205s2005 nju eng |
| 020 |
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|a 0471453293
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| 020 |
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|a 9780471453291
|
| 090 |
0 |
0 |
|a QH585.2
|b .F74 2005
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| 100 |
1 |
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|a Freshney, R. Ian ,
|e author
|
| 245 |
1 |
0 |
|a Culture of animal cells :
|b a manual of basic technique
|c R. Ian Freshney
|
| 250 |
|
|
|a 5th ed
|
| 260 |
|
|
|a Hoboken, NJ :
|b Wiley-Liss ,
|c c2005
|
| 300 |
|
|
|a 642 p. :
|b ill. (some col) ;
|c 29 cm.
|
| 504 |
|
|
|a Includes bibliographical references (p. 581-626) and index
|
| 505 |
0 |
|
|a 1. Introduction -- 2. Training programs -- 3. Biology of cultured cells -- 4. Laboratory design and layout -- 5. Equipment -- 6. Aseptic technique -- 7. Safety, bioethics, and validation -- 8. Culture vessels and substrates -- 9. Defined media and supplements -- 10. Serum-free media -- 11. Preparation and sterilization -- 12. Primary culture -- 13. Subculture and cell lines -- 14. Cloning and selection -- 15. Cell separation -- 16. Characterization -- 17. Differentiation -- 18. Transformation and immortalization -- 19. Contamination -- 20. Cryopreservation -- 21. Quantitation -- 22. Cytotoxicity -- 23. Culture of specific cell types -- 24. Culture of tumor cells -- 25. Organotypic culture -- 26. Scale-up -- 27. Specilized techniques -- 28. Problem solving -- 29. Conclusion
|
| 650 |
|
0 |
|a Cell culture
|v Laboratory manuals
|
| 650 |
|
0 |
|a Tissue culture
|v Laboratory manuals
|
| 999 |
|
|
|a 1000116494
|b Book
|c OPEN SHELF (30 DAYS)
|e Tembila Campus
|