Flow cytometry and cell sorting
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Berlin :
Springer ,
c2000
|
| Edition: | 2nd ed |
| Series: | Springer lab manual
|
| Subjects: | |
| Online Access: | Table of contents only Publisher description |
Table of Contents:
- Operation of a flow cytometer
- Conjugation of fluorochromes, haptens, and phycobiliproteins to antibodies
- Immunofluorescence: basic considerations
- Multicolor immunofluorescence analysis
- Combined intracellular and surface staining
- Scatchard analysis by flow cytometry
- Preparation and staining of cells for high-resolution DNA analysis
- Simultaneous flow cytometric detection of bromodeoxyuridine incorporation and cell surface marker expression
- High-resolution cell cycle analysis: the Flow Cytometric Bromodeoxyuridine
- Hoechst Quenching Technique
- Cell activation: Indo-1 ratiometric analysis of intracellular ionized calcium
- Biochemical parameters of cell function
- Molecular analysis of transcriptional control : the FACS-Galactosidase assay
- Ligand acidification by nonadherent cells
- Powerful preselection
- High gradient magnetic cell
- Sorting
- Setup of a flow sorter
- Sorting of rare cells
- Fluorescence-activated chromosome sorting
- Flow cytometry and sorting of plant chromosomes
- Large particle sorting
- Biological and laser safety