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20080619.0 |
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080205s2000 gw a b 001 0 eng |
| 020 |
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|a 3540656308 (wire-o-bind)
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| 020 |
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|a 9783540656302
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| 090 |
0 |
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|a QH585.5.F56
|b F55 2000
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| 245 |
0 |
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|a Flow cytometry and cell sorting
|c Andreas Radbruch, ed
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| 250 |
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|a 2nd ed
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| 260 |
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|a Berlin :
|b Springer ,
|c c2000
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| 300 |
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|a xxiii, 355 p. :
|b ill. ;
|c 24 cm.
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| 440 |
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0 |
|a Springer lab manual
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| 504 |
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|a Includes bibliographical references and index
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| 505 |
0 |
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|a Operation of a flow cytometer -- Conjugation of fluorochromes, haptens, and phycobiliproteins to antibodies -- Immunofluorescence: basic considerations -- Multicolor immunofluorescence analysis -- Combined intracellular and surface staining -- Scatchard analysis by flow cytometry -- Preparation and staining of cells for high-resolution DNA analysis -- Simultaneous flow cytometric detection of bromodeoxyuridine incorporation and cell surface marker expression -- High-resolution cell cycle analysis: the Flow Cytometric Bromodeoxyuridine -- Hoechst Quenching Technique -- Cell activation: Indo-1 ratiometric analysis of intracellular ionized calcium -- Biochemical parameters of cell function -- Molecular analysis of transcriptional control : the FACS-Galactosidase assay -- Ligand acidification by nonadherent cells -- Powerful preselection -- High gradient magnetic cell -- Sorting -- Setup of a flow sorter -- Sorting of rare cells -- Fluorescence-activated chromosome sorting -- Flow cytometry and sorting of plant chromosomes -- Large particle sorting -- Biological and laser safety
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| 650 |
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0 |
|a Flow cytometry
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| 700 |
1 |
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|a Radbruch, A. [
|q (Andreas)] ,
|e author
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| 856 |
4 |
1 |
|3 Table of contents only
|u http://www.loc.gov/catdir/enhancements/fy0812/99040869-t.html
|
| 856 |
4 |
2 |
|3 Publisher description
|u http://www.loc.gov/catdir/enhancements/fy0812/99040869-d.html
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| 999 |
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|a 1000116493
|b Book
|c OPEN SHELF (30 DAYS)
|e Tembila Campus
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| 999 |
|
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|a 1000130391
|b Book
|c OPEN SHELF (30 DAYS)
|e Tembila Campus
|