Cell imaging techniques : methods and protocols

Bibliographic Details
Main Authors: Mossman, Brooke T. , 1947- (Author), Taatjes, Douglas J. (Author)
Format: Book
Language:English
Published: Totowa, NJ : Humana Press , c2006
Series:Methods in molecular biology 319
Subjects:
Table of Contents:
  • Molecular beacons: fluorescent probes for detection of endogenous mRNAs in living cells
  • Second-harmonic imaging of collagen
  • Visualizing calcium signaling in cells by digitized wide-field and confocal fluorescent microscopy
  • Multifluorescence labeling techniques and confocal laser scanning microscopy on lung tissue
  • Evaluation of confocal microscopy system performance
  • Quantitative analysis of atherosclerotic lesion composition in mice
  • Applications of microscopy to genetic therapy of cystic fibrosis and other human diseases
  • Laser scanning cytometry: principles and applications
  • Near-clinical applications of laser scanning cytometry
  • Laser capture microdissection
  • Analysis of asbestos-induced gene expression changes in bronchiolar epithelial cells using laser capture microdissection and quantitative reverse transriptase-polymerase chain reaction
  • New approaches to fluorescence in situ hybridization
  • Microarray image scanning
  • Near-field scanning optical microscopy in cell biology and cytogenetics
  • Porosome: the fusion pore revealed by multiple imaging modalities
  • Secretory vesicle swelling by atomic force microscopy
  • Imaging and probing cell mechanical properties with the atomic force microscopy
  • Reflection contrast microscopy: the bridge between light and electron microscopy
  • Three-dimensional analysis of single particles by electron microscopy: sample preparation and data acquisition
  • Three-dimensional reconstruction of single particles in electron microscopy: image processing
  • A new microbiopsy system enables rapid preparation of tissue for high-pressure freezing