Search Results - "somatic cell nuclear transfer"

Manipulation of oocyte maturation to improve porcine somatic cell nuclear transfer by Chen, Wenchao

“…Porcine somatic cell nuclear transfer (SCNT) offers new opportunities for fundamental science, medicine and agriculture. …”
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Production of caprine cloned embryos using somatic cell nuclear transfer / Siti Haslinda Mohd Sharif by Siti Haslinda , Mohd Sharif

“…Production of cloned goat embryos using interspecies somatic cell nuclear transfer (SCNT) technique could be applied using cattle oocyte as recipient cytoplast since the source of goat oocytes is limited in our country. …”
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Development of intra- and interspecies somatic cell nuclear transfer protocols using ear fibroblast cells as donor karyoplasts for production of cloned caprine embryos / Kwong Phek Jin by Kwong, Phek Jin

“…Thus, this pioneering study was conducted with the aim to produce cloned caprine embryos through intraspecies somatic cell nuclear transfer (intraspSCNT) and interspecies somatic cell nuclear transfer (interspSCNT) techniques using caprine ear skin fibroblast cell as donor karyoplast as well as developing a protocol for caprine SCNT (intraspSCNT and interspSCNT). …”
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Production of cloned intra- and interspecies caprine embryos through somatic cell nuclear transfer technique with special reference to donor karyoplast type and recipient cytoplast source / Asdiana Amri by Asdiana, Amri

“…Therefore, using advanced assisted reproductive techniques (ART) through intra- and interspecies somatic cell nuclear transfer (SCNT) in order to produce cloned caprine embryos and subsequently offspring for propagation of genetically improved goat population at a rapid rate. …”
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Stress for stress tolerance? A fundamentally new approach in mammalian embryology by Pribenszky, C., Vajta, G., Molnar, M., Du, Y., Lin, L., Bolund, L., Yovich, John

“…HHP treatment of spermatozoa, oocytes, embryos, and embryonic stem cells increased fertilizing ability, developmental competence, and differentiation and improved results after cryopreservation, parthenogenetic activation, intracytoplasmic sperm injection, and somatic cell nuclear transfer. Osmotic stress of oocytes resulted in higher developmental rates after cryopreservation, parthenogenetic activation, and somatic cell nuclear transfer. …”
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Production of interspecies cloned caprine embryos and mouse embryonic fibroblast feeder cell layer to culture embryonic stem cell / Nurin Farhanah Norzaiwin by Nurin Farhanah , Norzaiwin

“…The extreme variability in efficiency of the interspecies somatic cell nuclear transfer showed improvement is needed to both procedures and resources used to produce interspecies cloned caprine embryos blastocysts prior subjected for production of embryonic stem cell on mouse embryonic fibroblast feeder cell layer. …”
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Genetic engineering advances and Qur’anic exegesis perspectives and challenges: the case of human cloning by Ibrahim , Esam Eltigani Mohamed

“…Cloning via somatic cell nuclear transfer is a process by which mammals are reproduced asexually. …”
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Nuclear transfer in ruminants by Lee, Joon-Hee, Maalouf, Walid E.

“…In this chapter, we describe in detail somatic cell nuclear transfer which is the foremost method for cloning ruminants with specific reference to sheep and cattle.…”
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Weighing human dignity and cloning from ethical and Islamic perspectives by Shuriye, Abdi Omar

“…This paper is on the ethics of cloning; the word cloning literally connotes a cell or group of cells, the process of human cloning may use a technique called Human somatic cell Nuclear Transfer. Clone also refers to organism descended asexually from a single ancestor. …”
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Healthy ageing of cloned sheep by Sinclair, Kevin D., Corr, Sandra A., Gutierrez, Carlos G., Fisher, Patricia A., Lee, J.-H., Rathbone, Alexandra J., Choi, Inchul, Campbell, Keith H.S., Gardner, David S.

“…The health of cloned animals generated by somatic-cell nuclear transfer (SCNT) has been of concern since its inception; however, there are no detailed assessments of late-onset, non-communicable diseases. …”
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Future of reproductive biotechnologies in water buffalo in Southeast Asian countries by Hufana-Duran, Danilda, Chaikhun-Marcou, Thuchadaporn, Duran, Peregrino G., Atabay, Eufrocina P., Nguyen, Hong Thi, Atabay, Edwin C., Nguyen, Uoc Thi, Nguyen, Hiep Thi, Hiew, Mark W.H., Punyawai, Kanchana, Ginting, Nurzainah, Parnpai, Rangsun

“…The application of multiple ovulation and embryo transfer (MOET) is constrained by poor embryo recovery in this livestock species. Somatic cell nuclear transfer (SCNT) offers great potential for producing sex-predetermined and genetically superior buffalo but requires further research to increase efficiency. …”
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One-carbon metabolism and epigenetic programming of mammalian development by Clare, Constance E

“…The observation that in vitro embryo culture and manipulation techniques, such as somatic cell nuclear transfer, can cause epigenetic alterations to DNA methylation during preimplantation development provides a basis for further study into the safety and efficacy of emerging assisted reproductive technologies.…”
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Production of cloned-caprine embryos in vitro obtained from interspecies nuclear transfer using bovine cytoplast and caprine karyoplast / Soh Hui Hui by Soh, Hui Hui

“…Production of cloned-caprine embryos using the intraspecies somatic cell nuclear transfer is limited by low source of caprine oocytes as the recipient cytoplast in Malaysia. …”
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Cryopreservation of ovine oocytes by Moawad, Adel Reda

“…In this thesis, recovery, viability and subsequent developmental potential following in vitro fertilisation (IVF), parthenogenetic activation or somatic cell nuclear transfer (SCNT) of ovine oocytes vitrified at GV stage and matured in vitro were studied. …”
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Effects of S-adenosyl-L-homocysteine and trichostatin A on developmental competence, epigenetic modification, and gene expression in cloned cattle embryos by Jafari, Shahram

“…With regards to low efficiency of the somatic cell nuclear transfer (SCNT)procedure the hypothesis that epigenetic marks of somatic donor cells are responsible for this inefficiency is proposed. …”
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Using mouse embryonic fibroblast (MEF) as feeder cells for production of embryonic stem cell (ESC) line in the murine and caprine / Goh Siew Ying by Goh, Siew Ying

“…In order to obtain the blastocyst sources for production of mESC and gESC lines, somatic cell nuclear transfer (in vitro) and in vivo flushing were carried out in this study. …”
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Improved in vitro development of cloned bovine embryos using S-Adenosylhomocysteine, a non-toxic epigenetic modifying reagent. by Jafari, Shahram, Hosseini, Morteza S., Hajian, Mahdi, Forouzanfar, Mohsen, Jafarpour, Farnoosh, Abedi, Parvaneh, Ostadhosseini, Somayyeh, Abbasi, Hassan, Gourabi, Hamid, Shahverdi, Abdol H., Taghi Dizaj, Ahmad Vosough, Anjomshoaa, Maryam, Haron, Abd Wahid, Nordin, Norshariza, Yaakub, Halimatun, Nasr Esfahani, Mohammad Hosein

“…The fluorescence intensities of the EGFP-POU5F1 reporter gene at all intervals of SAH treatment, except of 72hr, were significantly higher than control somatic cell nuclear transfers (SCNT) embryos. The intensity of DNA-methylation in cloned embryos treated with SAH for 48hr was similar to that of IVF embryos, and was significantly lower than the other SCNT groups. …”
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Improved in vitro development of cloned bovine embryos using S-adenosylhomocysteine, a non-toxic epigenetic modifying reagent. by Jafari, Shahram, Hosseini, Morteza S., Hajian, Mehdi, Forouzanfar, Mohsen, Jafarpour, Farnoosh, Abedi, Parvaneh, Ostadhosseini, Somayyeh, Abbasi, Hassan, Gourabi, Hamid, Shahverdi, Abdolhossein H., Dizaj, A. V. T., Anjomshoaa, Maryam, Haron, Wahid, Noor, Noorshariza, Yakub, Halimhatoon, Nasr Esfahani, Mohammad Hosein

“…The fluorescence intensities of the EGFP-POU5F1 reporter gene at all intervals of SAH treatment, except of 72 hr, were significantly higher than control somatic cell nuclear transfers (SCNT) embryos. The intensity of DNA-methylation in cloned embryos treated with SAH for 48 hr was similar to that of IVF embryos, and was significantly lower than the other SCNT groups. …”
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