Search Results - "protein crystallization"
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A step towards long-wavelength protein crystallography: Subjecting protein crystals to a vacuum
Published 2015“…Despite the lower data quality of the vacuum-pumped crystals compared with control crystals, it is demonstrated that the protein crystals can survive in a vacuum under suitable conditions.…”
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Crystal growth under microgravity condition
Published 2008“…The present invention relates to the growth of protein crystallization on earth and in zero-gravity conditions. …”
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Expression, purification and characterization of meta-cleavage enzyme CarBaBb froim Novosphiongobium sp. K
Published 2013“…Ka1 would also be a good candidate for protein crystallization and structural studies apart from carbabb from strain p. …”
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Novel fusion tags for protein production, purification and research applications
Published 2020“…The fusion proteins produced were also investigated for protein crystallization, but no crystals have been obtained till now. …”
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Elucidating the mechanism of action of novel lactams on Pseudomonas aeruginosa
Published 2019“…For P. aeruginosa, reporter gene fusions, in silico, iso-thermal titration calorimetry, circular dichroism spectroscopy, protein crystallization and X-ray diffraction studies have revealed that the most potent compound inhibit 2-alkyl-4-quinolone (AQ) dependent quorum sensing by binding to and inhibiting the response regulator protein, PqsR. …”
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Molecular identification and biodegradation of 3-chloropropionic acid (3CP) by filamentous fungi-Mucor and Trichoderma species isolated from UTM agricultural land
Published 2012“…Conclusion, significance and impact of study: Dehalogenase enzyme that can degrade α-chloro-substituted haloalkanoic acids for example 2,2DCP is well studied up to protein crystallization. Very few reports on the degradation of β-chloro-substituted haloalkanoic acids such as 3CP and none from fungi. …”
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Crystallization of novel ARM lipase and elucidation of its space-grown crystal structure
Published 2013“…Crystals grew in the gel-tube capillaries that are incubated in Protein Crystallization Research Facility on board the International Space Station for over three months in 2011. …”
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Structural determination of improved methanol-tolerant mutant from Geobacillus zalihae T1 lipase by x-ray crystallography
Published 2022“…Experimentally, the purified protein of mutant 2DC lipase was used to screen protein crystal for diffraction to elucidate and validate the mutant’s structure. …”
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Structural elucidation of thermostable L2 lipase based on ground and microgravity grown crystals
Published 2012“…Structural determination and comparison of microgravity and ground grown protein crystals have been carried out in order to investigate the effect of microgravity on the structure of protein crystals. …”
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Polymer-mediated crystallisation of proteins
Published 2015“…Understanding the structure and function of proteins is central to numerous areas of science and technology, including biotechnological, pharmaceutical and chemical industries. Protein crystals are an important objective for many researchers, but even though there are many examples of protein crystals reported, there are still many uncertainties in controlling the crystallisation process. …”
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Microbial enzymes: from earth to space
Published 2009“…The crystals are analyzed by x-ray diffraction to determine their structures, but this procedure is only possible for large and relatively pure proteins. As protein crystals are fragile, it is difficult for some proteins to grow adequately large or to obtain perfect protein crystals in Earth-based laboratories. …”
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Crystallographic analysis of ground- and space-grown thermostable T1 lipase crystal
Published 2013“…X-ray crystallography is a major tool to provide three dimensional structures. High resolution protein crystal data is important in revealing a highly accurate structure at atomic level. …”
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Crystallization and X-ray crystallographic analysis of recombinant TylP, a putative γ-butyrolactone receptor protein from Streptomyces fradiae
Published 2017“…The rTylP protein was crystallized from a reservoir solution comprising 34%(v/v) ethylene glycol and 5%(v/v) glycerol. The protein crystals diffracted X-rays to 3.05 Å resolution and belonged to the trigonal space group P3121, with unit-cell parameters a = b = 126.62, c = 95.63 Å.…”
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Crystallization and preliminary crystallographic studies of the hypothetical protein BPSL1038 from Burkholderia pseudomallei
Published 2014“…A pathogen-associated hypothetical protein, BPSL1038, was overexpressed in Escherichia coli, purified and crystallized using vapour-diffusion methods. A BPSL1038 protein crystal that grew using sodium formate as precipitant diffracted to 1.55 A ° resolution. …”
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Crystallization and preliminary crystallographic studies of the hypothetical protein BPSL 1038 from Burkholderia pseudomallei
Published 2014“…A pathogen-associated hypothetical protein, BPSL1038, was overexpressed in Escherichia coli, purified and crystallized using vapour-diffusion methods. A BPSL1038 protein crystal that grew using sodium formate as precipitant diffracted to 1.55 Å resolution. …”
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A comparative analysis of microgravity and earth grown thermostable T1 lipase crystals using HDPCG apparatus
Published 2015“…The crystallisation of T1lipase in space was carried out using a High-Density Protein Crystal Growth (HDPCG) apparatus with the vapour diffusion method, and X-ray diffraction data were collected. …”
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Molecular dynamic simulation of space and earth-grown crystal structures of thermostable T1 lipase geobacillus zalihae revealed a better structure
Published 2017“…Less sedimentation and convection in a microgravity environment has become a well-suited condition for growing high quality protein crystals. Thermostable T1 lipase derived from bacterium Geobacillus zalihae has been crystallized using the counter diffusion method under space and earth conditions. …”
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Molecular dynamic simulation of space and earth-grown crystal structures of thermostable T1 lipase Geobacillus zalihae revealed a better structure
Published 2017“…Less sedimentation and convection in a microgravity environment has become a well-suited condition for growing high quality protein crystals. Thermostable T1 lipase derived from bacterium Geobacillus zalihae has been crystallized using the counter diffusion method under space and earth conditions. …”
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Metabolomics combined with chemometric analysis to identify α-glucosidase inhibitors in Phaleria macrocarpa fruit extracts and its molecular docking simulation
Published 2024“…All the potential inhibitors identified were further docked to the yeast (Saccharomyces cerevisiae) protein crystal structure (PDB3A4A). The generated score scatter plot of OPLS showed a recognizable separation of all the extracts into six different clusters. …”
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