Search Results - "nucleic acid"
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Retroviral nucleic acid and method of use.
Published 2009“…The present invention provides a retroviral nucleic acid capable of tissue-specific expression of a heterologous nucleic acid in cell and uses thereof. …”
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Nucleic acid extraction from biomining microorganisms
Published 2009“…Various methods of nucleic acid (NA) extraction were investigated with the aim of developing a quantitative method of NA extraction from five representative strains of biomining microorganisms. …”
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Nanostructured Materials: Bioengineering Platforms for Sensing Nucleic Acids
Published 2016“…Detection of specific nucleic acid targets is of enormous and increasing interest in molecular biodiagnostics, food analysis, forensic investigation, and environmental monitoring. …”
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The recovery of nucleic acid from biomining and acid mine drainage microorganisms
Published 2011“…The different stages in the methods of NA extraction were investigated separately, including the: (i) removal of cells from pyrite, (ii) cell lysis and nucleic acid extraction, (iii) nucleic acid precipitation and (iv) RNA purification using, as appropriate, microscopy, spectrophotometry, agarose gel electrophoresis of NA, PCR, quantitative-PCR and quantitative reverse transcriptase-PCR to assess the quality and quantity of the DNA and RNA.The relative percentage of NA recovered from each microorganism using the optimised method discussed in this paper returned the following percentage of NA per cell: At. ferrooxidans 91% ± 1.4%; At. caldus 91% ± 2.7%; L. ferriphilum 98% ± 1.2%; F. acidiphilum 83% ± 2.9%; and S. thermosulfidooxidans 79% ± 0.7%. …”
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Development Of A Nucleic Acid Amplification Test For The Detection Of Mycobacterium Tuberculosis
Published 2015“…This shifts the attention towards the nucleic-acid based detection of M. tuberculosis, which requires the identification of suitable nucleic acid target as the diagnostic marker.…”
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Nucleic acid-based methods for on-site detection of plant pathogens : approaches and applications
Published 2012“…The ability to perform nucleic acid-based detection of plant pathogens away from conventional laboratory facilities has the potential to be beneficial in situations where results are required very rapidly or where resources and access to laboratory equipment are limited. …”
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New variants of poly(beta amino esters) to enhance delivery of nucleic acid medicines
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Comparative Analysis Of Nucleic Acid Residues In Core Streptavidin Using Three Isolation Techniques
Published 2024“…However, during IBs preparation, presence of cellular contaminants such as residual nucleic acids is inevitable and can affect subsequent refolding process. …”
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Unlocking the antibacterial potential of xanthone from calophyllum species: inhibition of nucleic acid synthesis
Published 2023“…Topoisomerase enzymes (DNA gyrase and topoisomerase IV) are DNA metabolism enzymes that possess distinct roles as unlinking enzymes during DNA replication. Nucleic acid synthesis inhibition reduces bacteria proliferation through the inhibition of topoisomerase enzymes that are essential for bacterial growth. …”
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Development of paper-based biosensor for point-of-care- nucleic acid testing / Choi Jane Ru
Published 2016“…Nucleic acid testing (NAT), a molecular diagnostic technique that involves nucleic acid extraction, amplification and detection, conventionally relies on well-established laboratories, high-end instrumentation and highly trained operators, limiting its use in resource-poor settings, where most diseases exist. …”
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Development of simple nucleic acid diagnostic tools for rapid and reliable diagnosis of Candida spp. infections
Published 2019“…Here, two different biomarker detection platforms were explored, namely, Multi-component Nucleic Acid Enzymes (MNAzymes) and Multiplex Probe Amplification (MPA), as simple nucleic acid biosensors for the diagnosis of invasive candidiasis. …”
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