Search Results - "genomic library"
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Genomic library construction of metroxylon sagu
Published 2005“…The genomic library construction is based on the principle of the restriction endonuclease partial digestion by using I unit of enzyme per Jlg of DNA. …”
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Tissue selection and optimization of DNA extraction for the construction of genomic library of white dragon fruit (Hylocereus undatus) / Loo Zhang Xin
Published 2011“…Genomic library of Hylocereus undatus was successfully constructed using Lambda Bacteriophage FIX II vector kit (Stratagene) which resulted in titer of 4 x 104 pfu/ml. …”
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Tissue selection and the optimization of the DNA isolation method for the construction of genomic library of red dragon fruit (Hylocereus polyrhizus) / Daniel Lee Kian Poh
Published 2013“…Genomic library of Hylocereus polyrhizus was constructed in bacteriophage Lambda Fix II with 20kb inserts. …”
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The Investigation of Sex Specific DNA Regions in Morelia spilota imbricata
Published 2018“…This includes one RAPD fragment, seven DNA region identified using RDA, genomic DNA representing 13 genes localised to the snake Z chromosome and the construction of one male and one female genomic library. This research could not resolve their sex determination system.…”
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Characterization of polymorphic microsatellite loci in the western rock lobster (Panulirus cygnus)
Published 2009“…The first method involved the screening of a small, fragment, partial genomic library with a radioactive (CA) 6 probe. …”
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Novel lipase gene from Bacillus sphaericus 205y
Published 2009“…The lipase gene was isolated via genomic library and sequenced. The production of lipase gene from Bacillus sphaericus strain 205Y is a novel gene. …”
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Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii.
Published 2011“…Seven single locus microsatellite markers were characterized in Malaysian giant freshwater prawn, Macrobrachium rosenbergii from an enriched genomic library Primer pairs were designed to flank the repeat sequences and the loci characterized for this species. …”
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Development and characterization of Elaeis oleifera microsatellite markers
Published 2010“…Ten Elaeis oleifera microsatellite markers were developed and characterised from 1500 sequences of the E. oleifera genomic library. The markers were utilised to assess the genetic diversity of E. oleifera germplasm collections from four South American countries (Colombia, Costa Rica, Panama and Honduras). …”
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Expression of Reverse Transcriptase Genes in Fulvia fulva
Published 1989“…We report here the DNA sequence of a 225-bp clone from a ?gt11 genomic library of F. fulva. This clone, designated P5, exhibits a high degree of sequence homology with the reverse transcriptase (pol) gene of the Drosophila melanogaster copia-like retrotransponson 17.6. …”
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Development Of Microsatellite Markers And Genetic Diversity Assessment Of Kempas (Koompassia Malaccensis) In Peninsular Malaysia
Published 2009“…The microsatellite primers were designed from a genomic library enriched for dinucleotide (CT) repeats and subsequently screened on 24 samples from a natural population. …”
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DNA fingerprinting and minisatellite variation of swans
Published 1990“…DNA fingerprinting has been used to study mating behaviour of Mute and Whooper Swans in the wild The results showed that the Whooper swans were almost strictly monogamous and Mute Swans exhibited an adaptable reproductive system. A genomic library from Cygnus olor was constructed and dozens of minisatellites were isolated. …”
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Isolation and characterization of microsatellite loci in the Malaysian giant freshwater prawn, Macrobrachium rosenbergii
Published 2008“…These microsatellites were isolated from an enriched genomic library contained by using a 5′‐anchored polymerase chain reaction technique. …”
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Molecular Cloning, Sequencing and Expression of an Organic Solvent-Tolerant Lipase Gene from Bacillus Sphaericus 205Y
Published 2002“…The gene coding for the extracellular lipase of Bacillus sphaericus 205y was isolated via genomic library and cloned into pUC 19.…”
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Isolation and development of DNA microsatellite markers for the river catfish (Mystus nemurus)
Published 2007“…A degenerate primer was designed for the construction of the genomic library. Fourteen clones were sequenced and revealed a total of 45 microsatellite repeats. …”
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Cloning and expression of a novel lipase gene from Bacillus sphaericus 205y
Published 2003“…The gene for the lipase was recovered from a genomic library and sequenced. Phylogenetic analysis was performed based on an alignment of thirteen microbial lipase sequences obtained from the NCBI database. …”
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Genomic cloning and identification of a novel murine Cyp4a gene
Published 1997“…Additionally, a lambda genomic library was screened with probes derived from the partial cDNA clones of the mouse cyp4a10 and cyp4a12. …”
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Genetic analysis of radiation resistance in Haloferax volcanii
Published 2024“…In the work presented here, the gene encoding a XerCD-like integrase, found within the integrated prophage on the main chromosome of Haloferax volcanii was isolated from a genomic library and overexpression leads to increased resistance to genotoxic stress imposed by ultraviolet light and mitomycin C. …”
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