Search Results - "enzyme kinetics"

Kinetics, crystallization, and structural elucidation of thermostable D311E T1 lipase variant from Geobacillus zalihae by Ruslan, Rudzanna

Subjects: “…Enzyme kinetics…”
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Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation by Norazwina, Zainol, Siti Natrah, Ismail

“…In this study, the parameters of the enzyme's kinetic, KM and Vmax were directly determined using a modified Michaelis-Menten equation by applying the Lineweaver-Burk plots. …”
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Empirical evaluation of a virtual laboratory approach to teach lactate dehydrogenase enzyme kinetics by Booth, C., Cheluvappa, R., Bellinson, Z., Maguire, D., Zimitat, Craig, Abraham, J., Eri, R.

“…Pertinent specific aims were to:. (1)design/implement a web-based lesson to teach lactate dehydrogenase(LDH) enzyme kinetics to 2nd-year biochemistry students(2)determine its efficacious in improving students' comprehension of enzyme kinetics(3)assess their perception of its usefulness/manageability(vLab versus Conventional Tutorial). …”
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An investigation into the drug disposition of the spleen organ as a potential immuno-therapeutic target by Inuwa, Patience G.

Subjects: “…Spleen; Therapeutics; Immune response; Drug disposition; Enzyme kinetics; Drug metabolism; Immunotherapy…”
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Identification of lipases with activity towards monoacylglycerol by criterion of conserved cap architectures by Riegler-Berket, Lina, Leitmeier, Andrea, Aschauer, Philipp, Dreveny, Ingrid, Oberer, Monika

Subjects: “…Monoacylglycerol lipase; Monoglyceride lipase; Lipase cap; Conserved cap architecture; Lipase lid; Enzyme kinetics…”
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Biopolymeric formulations for biocatalysis and biomedical applications by Elnashar, Magdy, Kahil, T.

“…The carriers have been used to immobilize lactase covalently and it was evaluated in terms of enzyme loading capacity and enzyme kinetics (km and Vmax). The Km of the Chito formulation was almost half that of the PEI formulations, which is favored in industries. …”
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Modelling the kinetics of seedless guava(Psidium guajava L.) peroxidase inactivation due to heat and thermosonication treatments. by Ganjloo, A., Abdul Rahman, Russly, Bakar, Jamilah, Osman, Azizah, Bimakr, Mandana

“…In both treatments, the enzyme kinetics showed a first-order kinetics model with monophasic behaviour. …”
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Properties of immobilized candida antarctica lipase B on highly macroporous copolymer by Nurrahmi Handayani, Nemanja Miletic, Katja Loos, Sadijah Achmad, Bumbun Bundjali

“…More over, the aim of this procedure is to improve the activity and reusability of lipase. Enzyme kinetics test was carried out by transesterification reaction between 4-nitrophenyl acetate (pNPA) and methanol by varying substrate concentrations, and the result is immobilized enzymes follows the Michaelis-Menten models and their activity is match with previous experiment. …”
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Characterization and thermostability study of invertase by Aspergillus niger in submerged culture by Nurul Izzah, Ahmad

“…Invertase is a commercially important enzyme used for hydrolysis of sucrose.The hydrolysis of sucrose yields a mixture of glucose and fructose, or famous as name invert syrup, this enzyme was widely used in food and beverage industries.Objectives of this research are to study the pH and thermostability of invertase by Aspergillus niger,to study the enzyme kinetics of invertase and to study the effect of sucrose concentration during incubation towards invertase activity.A.niger produced high levels of invertase under culture conditions (potato dextrose agar) on fourth day of incubation at an optimum temperature 30OC,inoculum at 30OC at 250 rpm and using sucrose as a substrate by submerged fermentation(SmF)also with same culture conditions.Separation between enzyme and other cell was done by centrifugation and continued with study the pH and thermostability of enzyme by terminated by heating. …”
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Inhibition and kinetic studies of cellulose - and hemicellulose - degrading enzymes of Ganoderma boninense by naturally occurring phenolic compounds by A., Surendran, Y., Siddiqui, N. S., Ali, S., Manickam

“…METHODS AND RESULTS: Ten naturally occurring phenolic compounds were assessed for their inhibitory effect on the hydrolytic enzymes of G. boninense. The enzyme kinetics (Vmax and Km ) and the stability of the hydrolytic enzymes were also characterized. …”
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In Vitro Inhibition Of Herbal Constituents On Ugt2b7 Enzyme Activity by Abdullah, Nurul Huda

“…The formation of ZDV glucuronide (ZDVG) from ZDV by HLM and RLM was determined using a validated high performance liquid chromatography method. The enzyme kinetics parameters of zidovudine glucuronidation which are Vmax and Km values in HLM were 1450 ± 26.06 pmol/mg/min and 0.88 ± 0.05 mM respectively with an intrinsic clearance value (Vmax/Km) of 1.65 μL/mg/min.…”
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Comparison of the structure and activity of glycosylated and asglycosylated human carboxylesterase 1 by Arena de Souza, Victoria, Scott, David J., Nettleship, Joanne E., Rahman, Nahid, Charlton, Michael H., Walsh, Martin A., Owens, Raymond J.

“…The purified aglycosylated enzyme was found to be more active than glycosylated hCES1 and analysis of enzyme kinetics revealed that both enzymes exhibit positive cooperativity. …”
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Characterization and multi-step transketolase-ω- transaminase bioconversions in an immobilized enzyme microreactor (IEMR) with packed tube by Abdul Halim, Amanatuzzakiah, Szita, Nicolas, Baganz, Frank

“…The ability to synthesize a chiral pharmaceutical intermediate, ABT in relatively short time proves this IEMR system as a powerful tool for construction and evaluation of de novo pathways as well as for determination of enzyme kinetics.…”
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Activation of 2′ 5′-oligoadenylate synthetase by stem loops at the 5′-end of the West Nile virus genome by Deo, Soumya, Patel, Trushar R., Dzananovic, Edis, Booy, Evan P., Zeid, Khalid, McEleney, Kevin, Harding, Stephen E., McKenna, Sean A.

“…This region contains three RNA stem loops (SLI, SLII, and SLIII) whose relative contribution to OAS1 binding affinity and activation were investigated using electrophoretic mobility shift assays and enzyme kinetics experiments. Stem loop I, comprising nucleotides 1-73, is dispensable for maximum OAS1 activation, as a construct containing only SLII and SLIII was capable of enzymatic activation. …”
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Development of an inhibitive enzyme assay for heavy metal detection using molybdenum-reducing enzyme from Staphylococcus sp. isolate liz1 by Abu Bakar, Nurlizah

“…Characterization of Mo-reducing enzyme kinetics gave the Km and Vmax values of 5.722 mM and 127.1 nmole Mo-blue/min/mg protein for laboratory-prepared phosphomolybdate (LPPM), and 15.58 mM and 152.8 nmole Mo-blue/min/mg protein for NADH, respectively. …”
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The Influence Of Functionalized Sba-15 On The Production Of Citronellol Ester Via Immobilized Candida Rugosa Lipase by Ani, Yasmin Che

“…Therefore, the characterization of immobilized CRL on pure SBA-15 and APTES-SBA-15 mesoporous support has been investigated using FT-IR, XRD, SEM, TEM, EA and nitrogen adsorptiondesorption analysis, The enzyme kinetics mechanism for CRL-APTES-SBA-15 follows Ping Pong Bi Bi with noncompetitive inhibition compared to CRL-SBA-15 follows Ordered Bi Bi with uncompetitive inhibition. …”
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Enzyme characterisation and kinetic modelling of the pentose phosphate pathway in yeast by Messiha, Hanan L., Kent, Edward, Malys, Naglis, Carroll, Kathleen M., Swainston, Neil, Mendes, Pedro, Smallbone, Kieran

“…The combination of these two models was made possible by the standardised enzyme kinetic experiments carried out in both studies. …”
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Penggunaan elektrod filem nipis emas dalam analisis enzim secara konduktometri by Engku Norbaya Engku Muda, Burhanuddin Yeop Majlis, Mohd Aman Abu Bakar

“…Its application as an analytical tool was shown in determination of Michaelis constants in enzyme kinetic study. Urease was chosen as a model enzyme and urea was used as a substrate. …”
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Synthesis and investigation of anti-tyrosinase and antioxidant activity of new halogenated xanthone derivatives by Baharuddin, Fatin Farhana

“…Their half-maximal inhibitory concentration (IC50), enzyme kinetic analysis and antioxidant properties were determined. …”
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Xanthine oxidase inhibitory activity of methanolic extract of Alternanthera sessilis by Chong, Sharmaine, Loh, Khye Er

“…In the present study, hydromethanolic extract of red and green sessile joyweed, Alternanthera sessilis was evaluated for its in vitro XO inhibitory potential. Enzyme kinetic was determined using Lineweaver-Burk plot. …”
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