Search Results - "IEX"

Characterization, efficacy and toxicity potential of chlorogenic acid and protocatechuic acid-layered double hydroxide nanohybrids on a cancer model by Abd Gani, Shafinaz

“…Thus, PCA-ZnAl-IEX and HepG2 cells were used in the subsequent studies. …”
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Enhancement of a protocol purifying T1 lipase through molecular approach by Che Hussian, Che Haznie Ayu, Raja Abdul Rahman, Raja Noor Zaliha, Leow, Adam Thean Chor, Salleh, Abu Bakar, Mohamad Ali, Mohd Shukuri

“…T1 Lipase is a thermostable secretary protein of Geobacillus zalihae strain previously expressed in a prokaryotic system and purified using three-step purification: affinity 1, affinity 2, and ion exchange chromatography (IEX). This approach is time consuming and offers low purity and recovery yield. …”
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Design and simulate RISC-V processor using verilog by Ngu, David Teck Joung

“…The project is started by implementing several main modules, such as alu, aludec, maindec, imem, dmem, regfile, pc_mux, result_mux, pipeline register (IF/ID, ID/IEx, IEx/IMem, and IMem/IW), forwardMuxA and forwardMuxB. …”
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Study on adsorption behavior of alkaline protease on poly(ether sulfone) integrated with fish scale hydroxyapatite as self organized in ion exchange membrane by Jamali Sukaimi, Sofiah Hamzah, Nora’aini Ali, Mohd Sabri Mohd Ghazali

“…This study was performed to investigate the physiochemical effect on the protease adsorption produced by Bacillus sp. using highly specific ion exchange membrane (IExM). Effects of pH solution (adjusted with HCl and NaOH), ionic strength (adjusted by NaCl), and initial concentration on adsorption study were performed by using affinity polyethersulfone (PES) membrane integrated with fish scale hydroxyapatite (FSHAp), FSHAp/PES IExM. …”
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Preparation of Ion Exchange Membrane Chromatography by Modification of Polyethersulfone Membrane Through UV Grafting of [2-(Acryloyloxy) Ethyl] Trimethyl Ammonium Chloride and Acry... by Wan Najibah, Mohamad

“…The effect of AETMA and AA monomer concentration from 1.5 M to 2.0 M was studied. The IEX membrane was characterized in term of degree of grafting, changed of functional group as well as protein binding capacity using pure bovine serum albumin. …”
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Gene expression, characterization and in silico studies of delta fatty acid desaturases by Abd Halim, Nur Farah Anis

“…Recombinant Δ12 FAD was successfully purified in two steps of chromatography: hydrophobic interaction chromatography (HIC) and ion exchange chromatography (IEX) with resin Butyl-S Sepharose 6 Fast Flow and SP Sepharose Fast Flow, respectively with a total protein yield of 1.55 mg/mL. …”
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Expression and purification of soluble bacterially-expressed human hexokinase II in E.coli system by Tanbin, Suriyea, Ahmad Fuad, Fazia Adyani

“…The expressed protein was then purified to homogeneity by a combination of Immobilized Metal Ion Affinity Chromatography (IMAC), size exclusion chromatography (SEC) and ion-exchange chromatography (IEX), resulting in pure bacterially-expressed HK2. …”
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Enhancement in t1 lipase purification recovery using the novel construct pgex4t1/his-t1 by Che Hussian, Che Haznie Ayu, Raja Abd Rahman, Raja Noor Zaliha, Leow, Adam Thean Chor, Salleh, Abu Bakar, Mohamad Ali, Mohd Shukuri, Latip, Wahhida

“…Previously, the GST-T1 lipase was purified through two chromatographic steps: affinity and ion exchange (IEX) but the recovery yield was only 33%. To improve the recovery yield to over 80%, the GST tag from the pGEX system was replaced with a poly-histidine at the N-terminal of the T1 lipase sequence. …”
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Purification and Characterisation of the 33-Kilodalton Outer Membrane Protein of Pasteurella Multocida Type 6:B by Zainal Abidin, Zamirah

“…Comparisons in terms of efficiency of purification and yield from the crude extract between modified cylindrical preparative SDSPAGE and diethylaminoethyl-ion exchange chromatography (DEAE-IEX) was investigated. It was found out that the former was more superior, being less tedious to be carried out and had lower LPS contamination. …”
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Expression of truncated chalcone synthase from Boesenbergia rotunda L. mansf. in Escherichia coli by Parmin, Nor Azizah

“…The recombinant truncated CHS was purified by using affinity chromatography and Ion-Exchange Chromatography (IEX). The expressed CHS protein had a molecular weight of about 21 kDa, a size that matched with the prediction by bioinformatic analysis. …”
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Cloning, purification and characterization of a novel cold-adapted GDSL esterase from Photobacterium sp. J15 by Shakiba, Mehrnoush Hadaddzadeh

“…The His-tagged recombinant GDSL esterase was purified in two steps chromatography; affinity chromatography using Cobalt Sepharose resin and ion exchange (IEX) chromatography using Q-Sepharose resin. After the first purification step, Trx-tag was removed from the recombinant GDSL esterase using thrombin. …”
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Characterization and crystallization of W200R protease by Muhd Noor, Noor Dina

“…W200R which was purified by using a series of purification of affinity chromatography, IEX and HIC was subjected to crystallization trial. …”
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Characterization and structural modification of cold-adapted, organic solvent stable lipase from Staphylococcus epidermidis AT2 produced in Escherichia coli by Ahmad Kamarudin, Nor Hafizah

“…The enzyme was purified by two-step chromatographic method; hydrophobic interaction chromatography (HIC) and ion exchange chromatography (IEX) with 47.1% recovery and 3.5-fold purification factor. …”
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