The effect of rotenone crude extract from derris elliptica on the larvicidal activity (mortality) of mosquito

Rotenone is a bio-active compound extracted from Derris elliptica (Tuba). It has long been used as biopesticide, which is more environmental friendly. Recently, new processing methods have been published on the extraction and usage of rotenone. The objective of this paper is to obtain the effectiven...

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Main Authors: Zubairi, Saiful Irwan, Sarmidi, M. R., Aziz, R. A., Latip, R., Said, J.
Format: Conference or Workshop Item
Language:English
Published: 2004
Subjects:
Online Access:http://eprints.utm.my/9800/
http://eprints.utm.my/9800/1/ZubairiSI2004_the_effect_of_rotenone_crude_extract.pdf
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author Zubairi, Saiful Irwan
Sarmidi, M. R.
Aziz, R. A.
Latip, R.
Said, J.
author_facet Zubairi, Saiful Irwan
Sarmidi, M. R.
Aziz, R. A.
Latip, R.
Said, J.
author_sort Zubairi, Saiful Irwan
building UTeM Institutional Repository
collection Online Access
description Rotenone is a bio-active compound extracted from Derris elliptica (Tuba). It has long been used as biopesticide, which is more environmental friendly. Recently, new processing methods have been published on the extraction and usage of rotenone. The objective of this paper is to obtain the effectiveness of Derris plant crude extract against mosquito larvae. Rotenone crude extract was extracted using Normal Soaking Extraction (NSE) method. Two different solvent ratios were used to extract rotenone which is: (A) Methyl chloride: methanol (1:1) and (B) Methyl chloride: methanol (1:9). One part of both crude extracts using solvent ratios A and B were concentrated further using the rotary evaporator at 40 0C and under 800 mbar vacuum pressures. All the samples (normal and concentrated) were subjected to larvicidal activity testing using mosquito larvae. The samples were diluted to 5 different concentrations (0.01 mg/ml, 0.02 mg/ml, 0.03 mg/ml, 0.04 mg/ml and 0.05 mg/ml) for the biological activity treatment (LC50). Six larvae of Aedes aegypti were used to test each samples. The study found that concentrated crude extract (B) using solvent ratio (1:1) gave optimum mortality of mosquito larvae of 83.33 % at 0.05 mg/ml after 5 hours post treatment.
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institution Universiti Teknologi Malaysia
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language English
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spelling utm-98002010-06-02T02:03:33Z http://eprints.utm.my/9800/ The effect of rotenone crude extract from derris elliptica on the larvicidal activity (mortality) of mosquito Zubairi, Saiful Irwan Sarmidi, M. R. Aziz, R. A. Latip, R. Said, J. TP Chemical technology QH301 Biology Rotenone is a bio-active compound extracted from Derris elliptica (Tuba). It has long been used as biopesticide, which is more environmental friendly. Recently, new processing methods have been published on the extraction and usage of rotenone. The objective of this paper is to obtain the effectiveness of Derris plant crude extract against mosquito larvae. Rotenone crude extract was extracted using Normal Soaking Extraction (NSE) method. Two different solvent ratios were used to extract rotenone which is: (A) Methyl chloride: methanol (1:1) and (B) Methyl chloride: methanol (1:9). One part of both crude extracts using solvent ratios A and B were concentrated further using the rotary evaporator at 40 0C and under 800 mbar vacuum pressures. All the samples (normal and concentrated) were subjected to larvicidal activity testing using mosquito larvae. The samples were diluted to 5 different concentrations (0.01 mg/ml, 0.02 mg/ml, 0.03 mg/ml, 0.04 mg/ml and 0.05 mg/ml) for the biological activity treatment (LC50). Six larvae of Aedes aegypti were used to test each samples. The study found that concentrated crude extract (B) using solvent ratio (1:1) gave optimum mortality of mosquito larvae of 83.33 % at 0.05 mg/ml after 5 hours post treatment. 2004-05 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/9800/1/ZubairiSI2004_the_effect_of_rotenone_crude_extract.pdf Zubairi, Saiful Irwan and Sarmidi, M. R. and Aziz, R. A. and Latip, R. and Said, J. (2004) The effect of rotenone crude extract from derris elliptica on the larvicidal activity (mortality) of mosquito. In: Proceeding of Symposium Biologi Kebangsaan ke-7, 18-20 May 2004, Awana Genting Highlands, Pahang.
spellingShingle TP Chemical technology
QH301 Biology
Zubairi, Saiful Irwan
Sarmidi, M. R.
Aziz, R. A.
Latip, R.
Said, J.
The effect of rotenone crude extract from derris elliptica on the larvicidal activity (mortality) of mosquito
title The effect of rotenone crude extract from derris elliptica on the larvicidal activity (mortality) of mosquito
title_full The effect of rotenone crude extract from derris elliptica on the larvicidal activity (mortality) of mosquito
title_fullStr The effect of rotenone crude extract from derris elliptica on the larvicidal activity (mortality) of mosquito
title_full_unstemmed The effect of rotenone crude extract from derris elliptica on the larvicidal activity (mortality) of mosquito
title_short The effect of rotenone crude extract from derris elliptica on the larvicidal activity (mortality) of mosquito
title_sort effect of rotenone crude extract from derris elliptica on the larvicidal activity (mortality) of mosquito
topic TP Chemical technology
QH301 Biology
url http://eprints.utm.my/9800/
http://eprints.utm.my/9800/1/ZubairiSI2004_the_effect_of_rotenone_crude_extract.pdf