Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate

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Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate

Chitosanolytic activity was monitored using chitosan impregnated with Remazol Brilliant Blue R dye (RBB). Low-yielding chitosan (1%, w/v) hydrolysis was modelled using Viscozyme® L (50%, w/w), a commercial mixed-glucanase, and was found to perform optimally at pH 4.5 and 50 °C. The chitosanase assay...

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Bibliographic Details
Main Authors:	Fen, Lee Lih, Md.Illias, Rosli, Kamaruddin, Kamarulzaman, Maskat, Mohd Yusof, Hassan, Osman
Format:	Article
Published:	Elsevier BV 2006
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/9084/

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