Enzymatic study of the Dehalogenases involved in biodegradation of a herbicide 3-chloropropionic acid (3cp) by a novel indigenous bacteria
3-chloropropionic acid is synthetic halogenated compounds used in herbicide. A bacterium isolated from a soil sample and characterised as Rhodococcus sp. by 16S rRNA analysis, was able to degrade and utilised 3-chloropropionate as the sole source of carbon and energy. This was supported by the abili...
| Main Authors: | , , , , |
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| Format: | Monograph |
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Faculty of Biosciences and Bioengineering
2008
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| Online Access: | http://eprints.utm.my/9065/ |
| Summary: | 3-chloropropionic acid is synthetic halogenated compounds used in herbicide. A bacterium isolated from a soil sample and characterised as Rhodococcus sp. by 16S rRNA analysis, was able to degrade and utilised 3-chloropropionate as the sole source of carbon and energy. This was supported by the ability of the bacterium to grow on 20 mM 3-chloropropionate with a doubling time of 11.72 hours. The utilisation of 3-chloropropionate was also confirmed by detection of 3-chloropropionate depletion in the medium using HPLC (High Performance Liquid Chromatography). Cell free extract of Rhodococcus sp. had an enzyme specific activity of 0.013 μmol Cl-/min/mg protein towards 3-chloropropionate. |
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